Enzymatic conversion of sunflower oil to biodiesel in a solvent-free system: Process optimization and the immobilized system stability
Samo za registrovane korisnike
2009
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The feasibility of using the commercial immobilized lipase from Candida antarctica (Novozyme 435) to synthesize biodiesel from sunflower oil in a solvent-free system has been proved. Using methanol as an acyl acceptor and the response surface methodology as an optimization technique, the optimal conditions for the transesterification has been found to be: 45 degrees C, 3% of enzyme based on oil weight, 3:1 methanol to oil molar ratio and with no added water in the system. Under these conditions, gt 99% of oil conversion to fatty acid methyl ester (FAME) has been achieved after 50 h of reaction, but the activity of the immobilized lipase decreased markedly over the course of repeated runs. In order to improve the enzyme stability, several alternative acyl acceptors have been tested for biodiesel production under solvent-free conditions. The use of methyl acetate seems to be of great interest, resulting in high FAME yield (95.65%) and increasing the half-life of the immobilized lipase b...y about 20.1 times as compared to methanol. The reaction has also been verified in the industrially feasible reaction system including both a batch stirred tank reactor and a packed bed reactor. Although satisfactory performance in the batch stirred tank reactor has been achieved, the kinetics in a packed bed reactor system seems to have a slightly better profile (93.6 +/- 3.75% FAME yield after 8-10 h), corresponding to the volumetric productivity of 48.5 g/(dm(3) h). The packed bed reactor has operated for up to 72 h with almost no loss in productivity, implying that the proposed process and the immobilized system could provide a promising solution for the biodiesel synthesis at the industrial scale.
Ključne reči:
Candida antarctica lipase / Biodiesel / Transesterification / Methyl acetate / Packed bed reactorIzvor:
Bioresource Technology, 2009, 100, 21, 5146-5154Izdavač:
- Elsevier Sci Ltd, Oxford
Finansiranje / projekti:
- Razvoj biotehnoloških postupaka za proizvodnju aditiva i novih formulacija za prehrambenu industriju (RS-MESTD-MPN2006-2010-20064)
DOI: 10.1016/j.biortech.2009.05.068
ISSN: 0960-8524
PubMed: 19540754
WoS: 000268742800033
Scopus: 2-s2.0-67650333363
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Ognjanović, Nevena AU - Bezbradica, Dejan AU - Knežević-Jugović, Zorica PY - 2009 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1431 AB - The feasibility of using the commercial immobilized lipase from Candida antarctica (Novozyme 435) to synthesize biodiesel from sunflower oil in a solvent-free system has been proved. Using methanol as an acyl acceptor and the response surface methodology as an optimization technique, the optimal conditions for the transesterification has been found to be: 45 degrees C, 3% of enzyme based on oil weight, 3:1 methanol to oil molar ratio and with no added water in the system. Under these conditions, gt 99% of oil conversion to fatty acid methyl ester (FAME) has been achieved after 50 h of reaction, but the activity of the immobilized lipase decreased markedly over the course of repeated runs. In order to improve the enzyme stability, several alternative acyl acceptors have been tested for biodiesel production under solvent-free conditions. The use of methyl acetate seems to be of great interest, resulting in high FAME yield (95.65%) and increasing the half-life of the immobilized lipase by about 20.1 times as compared to methanol. The reaction has also been verified in the industrially feasible reaction system including both a batch stirred tank reactor and a packed bed reactor. Although satisfactory performance in the batch stirred tank reactor has been achieved, the kinetics in a packed bed reactor system seems to have a slightly better profile (93.6 +/- 3.75% FAME yield after 8-10 h), corresponding to the volumetric productivity of 48.5 g/(dm(3) h). The packed bed reactor has operated for up to 72 h with almost no loss in productivity, implying that the proposed process and the immobilized system could provide a promising solution for the biodiesel synthesis at the industrial scale. PB - Elsevier Sci Ltd, Oxford T2 - Bioresource Technology T1 - Enzymatic conversion of sunflower oil to biodiesel in a solvent-free system: Process optimization and the immobilized system stability EP - 5154 IS - 21 SP - 5146 VL - 100 DO - 10.1016/j.biortech.2009.05.068 ER -
@article{ author = "Ognjanović, Nevena and Bezbradica, Dejan and Knežević-Jugović, Zorica", year = "2009", abstract = "The feasibility of using the commercial immobilized lipase from Candida antarctica (Novozyme 435) to synthesize biodiesel from sunflower oil in a solvent-free system has been proved. Using methanol as an acyl acceptor and the response surface methodology as an optimization technique, the optimal conditions for the transesterification has been found to be: 45 degrees C, 3% of enzyme based on oil weight, 3:1 methanol to oil molar ratio and with no added water in the system. Under these conditions, gt 99% of oil conversion to fatty acid methyl ester (FAME) has been achieved after 50 h of reaction, but the activity of the immobilized lipase decreased markedly over the course of repeated runs. In order to improve the enzyme stability, several alternative acyl acceptors have been tested for biodiesel production under solvent-free conditions. The use of methyl acetate seems to be of great interest, resulting in high FAME yield (95.65%) and increasing the half-life of the immobilized lipase by about 20.1 times as compared to methanol. The reaction has also been verified in the industrially feasible reaction system including both a batch stirred tank reactor and a packed bed reactor. Although satisfactory performance in the batch stirred tank reactor has been achieved, the kinetics in a packed bed reactor system seems to have a slightly better profile (93.6 +/- 3.75% FAME yield after 8-10 h), corresponding to the volumetric productivity of 48.5 g/(dm(3) h). The packed bed reactor has operated for up to 72 h with almost no loss in productivity, implying that the proposed process and the immobilized system could provide a promising solution for the biodiesel synthesis at the industrial scale.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Bioresource Technology", title = "Enzymatic conversion of sunflower oil to biodiesel in a solvent-free system: Process optimization and the immobilized system stability", pages = "5154-5146", number = "21", volume = "100", doi = "10.1016/j.biortech.2009.05.068" }
Ognjanović, N., Bezbradica, D.,& Knežević-Jugović, Z.. (2009). Enzymatic conversion of sunflower oil to biodiesel in a solvent-free system: Process optimization and the immobilized system stability. in Bioresource Technology Elsevier Sci Ltd, Oxford., 100(21), 5146-5154. https://doi.org/10.1016/j.biortech.2009.05.068
Ognjanović N, Bezbradica D, Knežević-Jugović Z. Enzymatic conversion of sunflower oil to biodiesel in a solvent-free system: Process optimization and the immobilized system stability. in Bioresource Technology. 2009;100(21):5146-5154. doi:10.1016/j.biortech.2009.05.068 .
Ognjanović, Nevena, Bezbradica, Dejan, Knežević-Jugović, Zorica, "Enzymatic conversion of sunflower oil to biodiesel in a solvent-free system: Process optimization and the immobilized system stability" in Bioresource Technology, 100, no. 21 (2009):5146-5154, https://doi.org/10.1016/j.biortech.2009.05.068 . .