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dc.creatorOgnjanović, Nevena
dc.creatorBezbradica, Dejan
dc.creatorKnežević-Jugović, Zorica
dc.date.accessioned2021-03-10T11:05:19Z
dc.date.available2021-03-10T11:05:19Z
dc.date.issued2009
dc.identifier.issn0960-8524
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/1431
dc.description.abstractThe feasibility of using the commercial immobilized lipase from Candida antarctica (Novozyme 435) to synthesize biodiesel from sunflower oil in a solvent-free system has been proved. Using methanol as an acyl acceptor and the response surface methodology as an optimization technique, the optimal conditions for the transesterification has been found to be: 45 degrees C, 3% of enzyme based on oil weight, 3:1 methanol to oil molar ratio and with no added water in the system. Under these conditions, gt 99% of oil conversion to fatty acid methyl ester (FAME) has been achieved after 50 h of reaction, but the activity of the immobilized lipase decreased markedly over the course of repeated runs. In order to improve the enzyme stability, several alternative acyl acceptors have been tested for biodiesel production under solvent-free conditions. The use of methyl acetate seems to be of great interest, resulting in high FAME yield (95.65%) and increasing the half-life of the immobilized lipase by about 20.1 times as compared to methanol. The reaction has also been verified in the industrially feasible reaction system including both a batch stirred tank reactor and a packed bed reactor. Although satisfactory performance in the batch stirred tank reactor has been achieved, the kinetics in a packed bed reactor system seems to have a slightly better profile (93.6 +/- 3.75% FAME yield after 8-10 h), corresponding to the volumetric productivity of 48.5 g/(dm(3) h). The packed bed reactor has operated for up to 72 h with almost no loss in productivity, implying that the proposed process and the immobilized system could provide a promising solution for the biodiesel synthesis at the industrial scale.en
dc.publisherElsevier Sci Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/20064/RS//
dc.rightsrestrictedAccess
dc.sourceBioresource Technology
dc.subjectCandida antarctica lipaseen
dc.subjectBiodieselen
dc.subjectTransesterificationen
dc.subjectMethyl acetateen
dc.subjectPacked bed reactoren
dc.titleEnzymatic conversion of sunflower oil to biodiesel in a solvent-free system: Process optimization and the immobilized system stabilityen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage5154
dc.citation.issue21
dc.citation.other100(21): 5146-5154
dc.citation.rankaM21
dc.citation.spage5146
dc.citation.volume100
dc.identifier.doi10.1016/j.biortech.2009.05.068
dc.identifier.pmid19540754
dc.identifier.rcubconv_3223
dc.identifier.scopus2-s2.0-67650333363
dc.identifier.wos000268742800033
dc.type.versionpublishedVersion


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