The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes
Samo za registrovane korisnike
2010
Autori
Vuković, Goran D.Tomić, Sergej
Marinković, Aleksandar
Radmilović, Velimir R.
Uskoković, Petar
Čolić, Miodrag
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Dapsone is an anti-microbial and anti-inflammatory drug. Water-dispersible dapsone-modified multi-wall carbon nanotubes (dap-MWCNTs) were prepared by chemical modification of the carboxyl groups introduced on the surface of the nanotubes using O-(7-aza-benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (N-HATU) and N,N-diisopropylethylamine (DIEA). The modification was confirmed by Fourier-transform infrared spectroscopy, transmission election microscopy and thermogravimetric analysis. The biological effect of dap-MWCNTs was tested using rat peritoneal macrophages (PMempty set). By confocal laser microscopy and flow cytometry, it was shown that the dap-MWCNTs were rapidly ingested by PMempty set as were the control, oxidized o-MWCNTs. Neither dap-MWCNTs at lower concentrations (up to 50 mu g/ml), nor o-MWCNTs, at equivalent concentrations, respectively affected the viability of PMempty set, while higher concentrations triggered apoptosis. Apoptosis of PMempty set induc...ed by the control, o-MWCNTs, was higher than that induced by dap-MWCNTs and it correlated with the induction of oxidative stress. In contrast, dap-MWCNTs did not trigger oxidative stress but caused apoptosis of PMempty set predominantly after prolonged cultivation (3 days). Although equivalent concentrations of soluble dapsone induced oxidative stress, they were anti-apoptotic. Cumulatively, the obtained results show the complexity of dap-MWCNT/PMempty set interactions and suggest that this complex could be investigated for the treatment of dapsone-sensitive intracellular microorganisms or inflammatory diseases responding to dapsone therapy.
Izvor:
Carbon, 2010, 48, 11, 3066-3078Izdavač:
- Pergamon-Elsevier Science Ltd, Oxford
Finansiranje / projekti:
- Sinteza funkcionalnih materijala sa kontrolisanom strukturom na molekularnom i nano nivou (RS-MESTD-MPN2006-2010-142006)
- Millitary Medical Academy, Belgrade Serbia [VMA/06-10/A5]
- US Department of EnergyUnited States Department of Energy (DOE) [DE-ACO2-05CH11231]
DOI: 10.1016/j.carbon.2010.04.043
ISSN: 0008-6223
WoS: 000279984600008
Scopus: 2-s2.0-79955015341
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Vuković, Goran D. AU - Tomić, Sergej AU - Marinković, Aleksandar AU - Radmilović, Velimir R. AU - Uskoković, Petar AU - Čolić, Miodrag PY - 2010 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1648 AB - Dapsone is an anti-microbial and anti-inflammatory drug. Water-dispersible dapsone-modified multi-wall carbon nanotubes (dap-MWCNTs) were prepared by chemical modification of the carboxyl groups introduced on the surface of the nanotubes using O-(7-aza-benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (N-HATU) and N,N-diisopropylethylamine (DIEA). The modification was confirmed by Fourier-transform infrared spectroscopy, transmission election microscopy and thermogravimetric analysis. The biological effect of dap-MWCNTs was tested using rat peritoneal macrophages (PMempty set). By confocal laser microscopy and flow cytometry, it was shown that the dap-MWCNTs were rapidly ingested by PMempty set as were the control, oxidized o-MWCNTs. Neither dap-MWCNTs at lower concentrations (up to 50 mu g/ml), nor o-MWCNTs, at equivalent concentrations, respectively affected the viability of PMempty set, while higher concentrations triggered apoptosis. Apoptosis of PMempty set induced by the control, o-MWCNTs, was higher than that induced by dap-MWCNTs and it correlated with the induction of oxidative stress. In contrast, dap-MWCNTs did not trigger oxidative stress but caused apoptosis of PMempty set predominantly after prolonged cultivation (3 days). Although equivalent concentrations of soluble dapsone induced oxidative stress, they were anti-apoptotic. Cumulatively, the obtained results show the complexity of dap-MWCNT/PMempty set interactions and suggest that this complex could be investigated for the treatment of dapsone-sensitive intracellular microorganisms or inflammatory diseases responding to dapsone therapy. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Carbon T1 - The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes EP - 3078 IS - 11 SP - 3066 VL - 48 DO - 10.1016/j.carbon.2010.04.043 ER -
@article{ author = "Vuković, Goran D. and Tomić, Sergej and Marinković, Aleksandar and Radmilović, Velimir R. and Uskoković, Petar and Čolić, Miodrag", year = "2010", abstract = "Dapsone is an anti-microbial and anti-inflammatory drug. Water-dispersible dapsone-modified multi-wall carbon nanotubes (dap-MWCNTs) were prepared by chemical modification of the carboxyl groups introduced on the surface of the nanotubes using O-(7-aza-benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (N-HATU) and N,N-diisopropylethylamine (DIEA). The modification was confirmed by Fourier-transform infrared spectroscopy, transmission election microscopy and thermogravimetric analysis. The biological effect of dap-MWCNTs was tested using rat peritoneal macrophages (PMempty set). By confocal laser microscopy and flow cytometry, it was shown that the dap-MWCNTs were rapidly ingested by PMempty set as were the control, oxidized o-MWCNTs. Neither dap-MWCNTs at lower concentrations (up to 50 mu g/ml), nor o-MWCNTs, at equivalent concentrations, respectively affected the viability of PMempty set, while higher concentrations triggered apoptosis. Apoptosis of PMempty set induced by the control, o-MWCNTs, was higher than that induced by dap-MWCNTs and it correlated with the induction of oxidative stress. In contrast, dap-MWCNTs did not trigger oxidative stress but caused apoptosis of PMempty set predominantly after prolonged cultivation (3 days). Although equivalent concentrations of soluble dapsone induced oxidative stress, they were anti-apoptotic. Cumulatively, the obtained results show the complexity of dap-MWCNT/PMempty set interactions and suggest that this complex could be investigated for the treatment of dapsone-sensitive intracellular microorganisms or inflammatory diseases responding to dapsone therapy.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Carbon", title = "The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes", pages = "3078-3066", number = "11", volume = "48", doi = "10.1016/j.carbon.2010.04.043" }
Vuković, G. D., Tomić, S., Marinković, A., Radmilović, V. R., Uskoković, P.,& Čolić, M.. (2010). The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes. in Carbon Pergamon-Elsevier Science Ltd, Oxford., 48(11), 3066-3078. https://doi.org/10.1016/j.carbon.2010.04.043
Vuković GD, Tomić S, Marinković A, Radmilović VR, Uskoković P, Čolić M. The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes. in Carbon. 2010;48(11):3066-3078. doi:10.1016/j.carbon.2010.04.043 .
Vuković, Goran D., Tomić, Sergej, Marinković, Aleksandar, Radmilović, Velimir R., Uskoković, Petar, Čolić, Miodrag, "The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes" in Carbon, 48, no. 11 (2010):3066-3078, https://doi.org/10.1016/j.carbon.2010.04.043 . .