Sodium periodate oxidized cotton yarn as carrier for immobilization of trypsin
Само за регистроване кориснике
2010
Аутори
Nikolić, TanjaKostić, Mirjana
Praskalo, Jovana
Pejić, Biljana
Petronijević, Živomir
Škundrić, Petar
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cotton yarn was first oxidized by sodium periodate to introduce aldehyde groups which were able to react with amino groups of trypsin to form Schiff's base, and result in cotton yarn immobilized trypsin. The effect of periodate oxidation on the chemical and physical properties of cotton yarn was evaluated by determining aldehyde group content, fineness and tensile strength of yarn. Measurements of protein load from Bradford assay and catalytic activity in hydrolysis of N-alpha-benzoyl-DL-arginine p-nitroanilide were made for the immobilized enzyme. The maximum amount of immobilized trypsin was 6.1 mg/g dried cotton yarn. Trypsin immobilized on oxidized cotton yarn retained 90% and 72% of the initial activity at 4 degrees C and 25 degrees C, respectively, over 60 days of storage in physiological solution.
Кључне речи:
Cotton yarn / Sodium periodate oxidation / Trypsin / Immobilization / Storage stabilityИзвор:
Carbohydrate Polymers, 2010, 82, 3, 976-981Издавач:
- Elsevier Sci Ltd, Oxford
Финансирање / пројекти:
- Развој биолошки-активних полисахаридних влакана и материјала као вештачких депоа протеина мале молекулске масе за различите медицинске намене (у терапијама хормонских поремећаја, вирусних инфекција, неуролошких и малигних обољења, ортопедији и стоматологији (RS-MESTD-MPN2006-2010-19009)
DOI: 10.1016/j.carbpol.2010.06.028
ISSN: 0144-8617
WoS: 000281524900064
Scopus: 2-s2.0-77955663711
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Nikolić, Tanja AU - Kostić, Mirjana AU - Praskalo, Jovana AU - Pejić, Biljana AU - Petronijević, Živomir AU - Škundrić, Petar PY - 2010 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1672 AB - Cotton yarn was first oxidized by sodium periodate to introduce aldehyde groups which were able to react with amino groups of trypsin to form Schiff's base, and result in cotton yarn immobilized trypsin. The effect of periodate oxidation on the chemical and physical properties of cotton yarn was evaluated by determining aldehyde group content, fineness and tensile strength of yarn. Measurements of protein load from Bradford assay and catalytic activity in hydrolysis of N-alpha-benzoyl-DL-arginine p-nitroanilide were made for the immobilized enzyme. The maximum amount of immobilized trypsin was 6.1 mg/g dried cotton yarn. Trypsin immobilized on oxidized cotton yarn retained 90% and 72% of the initial activity at 4 degrees C and 25 degrees C, respectively, over 60 days of storage in physiological solution. PB - Elsevier Sci Ltd, Oxford T2 - Carbohydrate Polymers T1 - Sodium periodate oxidized cotton yarn as carrier for immobilization of trypsin EP - 981 IS - 3 SP - 976 VL - 82 DO - 10.1016/j.carbpol.2010.06.028 ER -
@article{ author = "Nikolić, Tanja and Kostić, Mirjana and Praskalo, Jovana and Pejić, Biljana and Petronijević, Živomir and Škundrić, Petar", year = "2010", abstract = "Cotton yarn was first oxidized by sodium periodate to introduce aldehyde groups which were able to react with amino groups of trypsin to form Schiff's base, and result in cotton yarn immobilized trypsin. The effect of periodate oxidation on the chemical and physical properties of cotton yarn was evaluated by determining aldehyde group content, fineness and tensile strength of yarn. Measurements of protein load from Bradford assay and catalytic activity in hydrolysis of N-alpha-benzoyl-DL-arginine p-nitroanilide were made for the immobilized enzyme. The maximum amount of immobilized trypsin was 6.1 mg/g dried cotton yarn. Trypsin immobilized on oxidized cotton yarn retained 90% and 72% of the initial activity at 4 degrees C and 25 degrees C, respectively, over 60 days of storage in physiological solution.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Carbohydrate Polymers", title = "Sodium periodate oxidized cotton yarn as carrier for immobilization of trypsin", pages = "981-976", number = "3", volume = "82", doi = "10.1016/j.carbpol.2010.06.028" }
Nikolić, T., Kostić, M., Praskalo, J., Pejić, B., Petronijević, Ž.,& Škundrić, P.. (2010). Sodium periodate oxidized cotton yarn as carrier for immobilization of trypsin. in Carbohydrate Polymers Elsevier Sci Ltd, Oxford., 82(3), 976-981. https://doi.org/10.1016/j.carbpol.2010.06.028
Nikolić T, Kostić M, Praskalo J, Pejić B, Petronijević Ž, Škundrić P. Sodium periodate oxidized cotton yarn as carrier for immobilization of trypsin. in Carbohydrate Polymers. 2010;82(3):976-981. doi:10.1016/j.carbpol.2010.06.028 .
Nikolić, Tanja, Kostić, Mirjana, Praskalo, Jovana, Pejić, Biljana, Petronijević, Živomir, Škundrić, Petar, "Sodium periodate oxidized cotton yarn as carrier for immobilization of trypsin" in Carbohydrate Polymers, 82, no. 3 (2010):976-981, https://doi.org/10.1016/j.carbpol.2010.06.028 . .