Use of Candida rugosa lipase immobilized on sepabeads for the amyl caprylate synthesis: Batch and fluidized bed reactor study
2010
Autori
Šaponjić, Svetlana V.Knežević-Jugović, Zorica
Bezbradica, Dejan
Žuža, Milena
Saied, Omar Ali
Bošković-Vragolović, Nevenka
Mijin, Dušan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Lipase from Candida rugosa was covalently immobilized on Sepabeads EC-EP for application for amyl caprylate synthesis in an organic solvent system. Several solvents were tested in terms of biocatalyst stability and the best result was obtained with isooctane. The lipase-catalyzed esterification in the selected system was performed in batch and fluidized bed reactor systems. The influence of several important reaction parameters including temperature, initial water content, enzyme loading, acid/alcohol molar ratio, and time of addition of molecular sieves is carefully analyzed by means of an experimental design. Almost complete conversion ( gt 99%) of the substrate to ester could be performed in a batch reactor system, using lipase loading as low as 37 mg g(-1) dry support and in a relatively short time (24 hrs) at 37 degrees C, when high initial substrate molar ratio of 2.2 is used. Kinetics in a fluidized bed reactor system seems to still have a slightly better profile than in the ba...tch system (90.2% yields after 14 hrs). The fluidized bed reactor operated for up 70 hrs almost with no loss in productivity, implying that the proposed process and the immobilized system could provide a promising approach for the amyl caprylate synthesis at the industrial scale.
Ključne reči:
bioreactors / covalent immobilization / ester / non-aqueous system / optimization techniqueIzvor:
Electronic Journal of Biotechnology, 2010, 13, 6Izdavač:
- University of Catolica De Valparaiso, Valparaiso
Finansiranje / projekti:
- Razvoj biotehnoloških postupaka za proizvodnju aditiva i novih formulacija za prehrambenu industriju (RS-MESTD-MPN2006-2010-20064)
DOI: 10.2225/vol13-issue6-fulltext-8
ISSN: 0717-3458
WoS: 000288177100008
Scopus: 2-s2.0-79960870106
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Šaponjić, Svetlana V. AU - Knežević-Jugović, Zorica AU - Bezbradica, Dejan AU - Žuža, Milena AU - Saied, Omar Ali AU - Bošković-Vragolović, Nevenka AU - Mijin, Dušan PY - 2010 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1691 AB - Lipase from Candida rugosa was covalently immobilized on Sepabeads EC-EP for application for amyl caprylate synthesis in an organic solvent system. Several solvents were tested in terms of biocatalyst stability and the best result was obtained with isooctane. The lipase-catalyzed esterification in the selected system was performed in batch and fluidized bed reactor systems. The influence of several important reaction parameters including temperature, initial water content, enzyme loading, acid/alcohol molar ratio, and time of addition of molecular sieves is carefully analyzed by means of an experimental design. Almost complete conversion ( gt 99%) of the substrate to ester could be performed in a batch reactor system, using lipase loading as low as 37 mg g(-1) dry support and in a relatively short time (24 hrs) at 37 degrees C, when high initial substrate molar ratio of 2.2 is used. Kinetics in a fluidized bed reactor system seems to still have a slightly better profile than in the batch system (90.2% yields after 14 hrs). The fluidized bed reactor operated for up 70 hrs almost with no loss in productivity, implying that the proposed process and the immobilized system could provide a promising approach for the amyl caprylate synthesis at the industrial scale. PB - University of Catolica De Valparaiso, Valparaiso T2 - Electronic Journal of Biotechnology T1 - Use of Candida rugosa lipase immobilized on sepabeads for the amyl caprylate synthesis: Batch and fluidized bed reactor study IS - 6 VL - 13 DO - 10.2225/vol13-issue6-fulltext-8 ER -
@article{ author = "Šaponjić, Svetlana V. and Knežević-Jugović, Zorica and Bezbradica, Dejan and Žuža, Milena and Saied, Omar Ali and Bošković-Vragolović, Nevenka and Mijin, Dušan", year = "2010", abstract = "Lipase from Candida rugosa was covalently immobilized on Sepabeads EC-EP for application for amyl caprylate synthesis in an organic solvent system. Several solvents were tested in terms of biocatalyst stability and the best result was obtained with isooctane. The lipase-catalyzed esterification in the selected system was performed in batch and fluidized bed reactor systems. The influence of several important reaction parameters including temperature, initial water content, enzyme loading, acid/alcohol molar ratio, and time of addition of molecular sieves is carefully analyzed by means of an experimental design. Almost complete conversion ( gt 99%) of the substrate to ester could be performed in a batch reactor system, using lipase loading as low as 37 mg g(-1) dry support and in a relatively short time (24 hrs) at 37 degrees C, when high initial substrate molar ratio of 2.2 is used. Kinetics in a fluidized bed reactor system seems to still have a slightly better profile than in the batch system (90.2% yields after 14 hrs). The fluidized bed reactor operated for up 70 hrs almost with no loss in productivity, implying that the proposed process and the immobilized system could provide a promising approach for the amyl caprylate synthesis at the industrial scale.", publisher = "University of Catolica De Valparaiso, Valparaiso", journal = "Electronic Journal of Biotechnology", title = "Use of Candida rugosa lipase immobilized on sepabeads for the amyl caprylate synthesis: Batch and fluidized bed reactor study", number = "6", volume = "13", doi = "10.2225/vol13-issue6-fulltext-8" }
Šaponjić, S. V., Knežević-Jugović, Z., Bezbradica, D., Žuža, M., Saied, O. A., Bošković-Vragolović, N.,& Mijin, D.. (2010). Use of Candida rugosa lipase immobilized on sepabeads for the amyl caprylate synthesis: Batch and fluidized bed reactor study. in Electronic Journal of Biotechnology University of Catolica De Valparaiso, Valparaiso., 13(6). https://doi.org/10.2225/vol13-issue6-fulltext-8
Šaponjić SV, Knežević-Jugović Z, Bezbradica D, Žuža M, Saied OA, Bošković-Vragolović N, Mijin D. Use of Candida rugosa lipase immobilized on sepabeads for the amyl caprylate synthesis: Batch and fluidized bed reactor study. in Electronic Journal of Biotechnology. 2010;13(6). doi:10.2225/vol13-issue6-fulltext-8 .
Šaponjić, Svetlana V., Knežević-Jugović, Zorica, Bezbradica, Dejan, Žuža, Milena, Saied, Omar Ali, Bošković-Vragolović, Nevenka, Mijin, Dušan, "Use of Candida rugosa lipase immobilized on sepabeads for the amyl caprylate synthesis: Batch and fluidized bed reactor study" in Electronic Journal of Biotechnology, 13, no. 6 (2010), https://doi.org/10.2225/vol13-issue6-fulltext-8 . .