Production of Bioethanol by Simultaneous Saccharification and Fermentation of Corn Meal by Immobilized Yeast
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2010
Authors
Mojović, LjiljanaRakin, Marica
Vukašinović-Sekulić, Maja
Nikolić, Svetlana
Pejin, Jelena
Pejin, Dušanka
Article (Published version)
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The production of bioethanol by simultaneous saccharification and fermentation (SSF) of corn meal using immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied in this work. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. The kinetics of the SSF process with immobilized yeast was assessed with various initial glucose concentrations. In addition, the effect of media supplementation with the yeast activators such as mineral salts, ZnSO4. 7H(2)O and MgSO4. 7H(2)O and vitamins, Ca-pantothenate, biotin and myoinositol was investigated. The system was compared with the SSF system with free yeast. The immobilized system was found superior to the free system since higher ethanol concentration and process productivity were obtained. The maximum increase in ethanol concentration was achieved in immobilized system when mineral salts were added. In this case, the ethanol concentration of 9.70% (w/w) was achieve...d after 38 hours of fermentation. Addition of the magnesium and zinc contributed to the achievement of a high productivity of the batch SSF of corn meal with immobilized S. cerevisiae var. ellipsoideus yeast, while still preserving a physical and chemical stability of Ca-alginate gel beads. High productivity of the system was attributed to the cell protection from inhibition by ethanol that was achieved by immobilization, and the activation of the yeast metabolism that was accomplished by the minerals.
Source:
Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener, 2010, 21, 1333-1338Publisher:
- Aidic Servizi Srl, Milano
Funding / projects:
- Micromechanical criteria of damage and fracture (RS-MESTD-Basic Research (BR or ON)-174004)
- Povećanje efikasnosti proizvodnja bioetanola na obnovljivim sirovinama potpunim iskorišćavanjem sporednih proizvoda (RS-MESTD-MPN2006-2010-18002)
DOI: 10.3303/CET1021223
ISSN: 2283-9216
WoS: 000286978600223
Scopus: 2-s2.0-78650295921
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Mojović, Ljiljana AU - Rakin, Marica AU - Vukašinović-Sekulić, Maja AU - Nikolić, Svetlana AU - Pejin, Jelena AU - Pejin, Dušanka PY - 2010 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1736 AB - The production of bioethanol by simultaneous saccharification and fermentation (SSF) of corn meal using immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied in this work. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. The kinetics of the SSF process with immobilized yeast was assessed with various initial glucose concentrations. In addition, the effect of media supplementation with the yeast activators such as mineral salts, ZnSO4. 7H(2)O and MgSO4. 7H(2)O and vitamins, Ca-pantothenate, biotin and myoinositol was investigated. The system was compared with the SSF system with free yeast. The immobilized system was found superior to the free system since higher ethanol concentration and process productivity were obtained. The maximum increase in ethanol concentration was achieved in immobilized system when mineral salts were added. In this case, the ethanol concentration of 9.70% (w/w) was achieved after 38 hours of fermentation. Addition of the magnesium and zinc contributed to the achievement of a high productivity of the batch SSF of corn meal with immobilized S. cerevisiae var. ellipsoideus yeast, while still preserving a physical and chemical stability of Ca-alginate gel beads. High productivity of the system was attributed to the cell protection from inhibition by ethanol that was achieved by immobilization, and the activation of the yeast metabolism that was accomplished by the minerals. PB - Aidic Servizi Srl, Milano T2 - Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener T1 - Production of Bioethanol by Simultaneous Saccharification and Fermentation of Corn Meal by Immobilized Yeast EP - 1338 SP - 1333 VL - 21 DO - 10.3303/CET1021223 ER -
@article{ author = "Mojović, Ljiljana and Rakin, Marica and Vukašinović-Sekulić, Maja and Nikolić, Svetlana and Pejin, Jelena and Pejin, Dušanka", year = "2010", abstract = "The production of bioethanol by simultaneous saccharification and fermentation (SSF) of corn meal using immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied in this work. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. The kinetics of the SSF process with immobilized yeast was assessed with various initial glucose concentrations. In addition, the effect of media supplementation with the yeast activators such as mineral salts, ZnSO4. 7H(2)O and MgSO4. 7H(2)O and vitamins, Ca-pantothenate, biotin and myoinositol was investigated. The system was compared with the SSF system with free yeast. The immobilized system was found superior to the free system since higher ethanol concentration and process productivity were obtained. The maximum increase in ethanol concentration was achieved in immobilized system when mineral salts were added. In this case, the ethanol concentration of 9.70% (w/w) was achieved after 38 hours of fermentation. Addition of the magnesium and zinc contributed to the achievement of a high productivity of the batch SSF of corn meal with immobilized S. cerevisiae var. ellipsoideus yeast, while still preserving a physical and chemical stability of Ca-alginate gel beads. High productivity of the system was attributed to the cell protection from inhibition by ethanol that was achieved by immobilization, and the activation of the yeast metabolism that was accomplished by the minerals.", publisher = "Aidic Servizi Srl, Milano", journal = "Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener", title = "Production of Bioethanol by Simultaneous Saccharification and Fermentation of Corn Meal by Immobilized Yeast", pages = "1338-1333", volume = "21", doi = "10.3303/CET1021223" }
Mojović, L., Rakin, M., Vukašinović-Sekulić, M., Nikolić, S., Pejin, J.,& Pejin, D.. (2010). Production of Bioethanol by Simultaneous Saccharification and Fermentation of Corn Meal by Immobilized Yeast. in Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener Aidic Servizi Srl, Milano., 21, 1333-1338. https://doi.org/10.3303/CET1021223
Mojović L, Rakin M, Vukašinović-Sekulić M, Nikolić S, Pejin J, Pejin D. Production of Bioethanol by Simultaneous Saccharification and Fermentation of Corn Meal by Immobilized Yeast. in Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener. 2010;21:1333-1338. doi:10.3303/CET1021223 .
Mojović, Ljiljana, Rakin, Marica, Vukašinović-Sekulić, Maja, Nikolić, Svetlana, Pejin, Jelena, Pejin, Dušanka, "Production of Bioethanol by Simultaneous Saccharification and Fermentation of Corn Meal by Immobilized Yeast" in Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener, 21 (2010):1333-1338, https://doi.org/10.3303/CET1021223 . .