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Global and Deep Molecular Analysis of Microbiota Signatures in Fecal Samples From Patients With Irritable Bowel Syndrome

Authorized Users Only
2011
Authors
Rajilić-Stojanović, Mirjana
Biagi, Elena
Heilig, Hans G. H. J.
Kajander, Kajsa
Kekkonen, Riina A.
Tims, Sebastian
de Vos, Willem M.
Article (Published version)
Metadata
Show full item record
Abstract
BACKGROUND & AIMS: Irritable bowel syndrome (IBS) has been associated with disruptions to the intestinal microbiota, but studies have had limited power, coverage, and depth of analysis. We aimed to define microbial populations that can be used discriminate the fecal microbiota of patients with IBS from that of healthy subjects and correlate these with IBS intestinal symptom scores. METHODS: The microbiota composition was assessed by global and deep molecular analysis of fecal samples from 62 patients with IBS patients and 46 healthy individuals (controls). We used a comprehensive and highly reproducible phylogenetic microarray in combination with quantitative polymerase chain reaction. RESULTS: The intestinal microbiota of IBS patients differed significantly (P=.0005) from that of controls. The microbiota of patients, compared with controls, had a 2-fold increased ratio of the Firmicutes to Bacteroidetes (P=.0002). This resulted from an approximately 1.5-fold increase in numbers of Dor...ea, Ruminococcus, and Clostridium spp (P lt .005); a 2-fold decrease in the number of Bacteroidetes (P lt .0001); a 1.5-fold decrease in numbers of Bifidobacterium and Faecalibacterium spp (P lt .05); and, when present, a 4-fold lower average number of methanogens (3.50 x 10(7) vs 8.74 x 10(6) cells/g feces; P=.003). Correlation analysis of the microbial groups and IBS symptom scores indicated the involvement of several groups of Firmicutes and Proteobacteria in the pathogenesis of IBS. CONCLUSIONS: Global and deep molecular analysis of fecal samples indicates that patients with IBS have a different composition of microbiota. This information might be used to develop better diagnostics and ultimately treatments for IBS.

Keywords:
High-Throughput Analysis / Phylogenetic Microarray / Microflora / Enterotypes
Source:
Gastroenterology, 2011, 141, 5, 1792-1801
Publisher:
  • W B Saunders Co-Elsevier Inc, Philadelphia
Funding / projects:
  • Netherlands Organization of Scientific ResearchNetherlands Organization for Scientific Research (NWO)

DOI: 10.1053/j.gastro.2011.07.043

ISSN: 0016-5085

PubMed: 21820992

WoS: 000296512200044

Scopus: 2-s2.0-80054857288
[ Google Scholar ]
644
587
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1854
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
Institution/Community
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Rajilić-Stojanović, Mirjana
AU  - Biagi, Elena
AU  - Heilig, Hans G. H. J.
AU  - Kajander, Kajsa
AU  - Kekkonen, Riina A.
AU  - Tims, Sebastian
AU  - de Vos, Willem M.
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1854
AB  - BACKGROUND & AIMS: Irritable bowel syndrome (IBS) has been associated with disruptions to the intestinal microbiota, but studies have had limited power, coverage, and depth of analysis. We aimed to define microbial populations that can be used discriminate the fecal microbiota of patients with IBS from that of healthy subjects and correlate these with IBS intestinal symptom scores. METHODS: The microbiota composition was assessed by global and deep molecular analysis of fecal samples from 62 patients with IBS patients and 46 healthy individuals (controls). We used a comprehensive and highly reproducible phylogenetic microarray in combination with quantitative polymerase chain reaction. RESULTS: The intestinal microbiota of IBS patients differed significantly (P=.0005) from that of controls. The microbiota of patients, compared with controls, had a 2-fold increased ratio of the Firmicutes to Bacteroidetes (P=.0002). This resulted from an approximately 1.5-fold increase in numbers of Dorea, Ruminococcus, and Clostridium spp (P lt .005); a 2-fold decrease in the number of Bacteroidetes (P lt .0001); a 1.5-fold decrease in numbers of Bifidobacterium and Faecalibacterium spp (P lt .05); and, when present, a 4-fold lower average number of methanogens (3.50 x 10(7) vs 8.74 x 10(6) cells/g feces; P=.003). Correlation analysis of the microbial groups and IBS symptom scores indicated the involvement of several groups of Firmicutes and Proteobacteria in the pathogenesis of IBS. CONCLUSIONS: Global and deep molecular analysis of fecal samples indicates that patients with IBS have a different composition of microbiota. This information might be used to develop better diagnostics and ultimately treatments for IBS.
PB  - W B Saunders Co-Elsevier Inc, Philadelphia
T2  - Gastroenterology
T1  - Global and Deep Molecular Analysis of Microbiota Signatures in Fecal Samples From Patients With Irritable Bowel Syndrome
EP  - 1801
IS  - 5
SP  - 1792
VL  - 141
DO  - 10.1053/j.gastro.2011.07.043
UR  - conv_3686
ER  - 
@article{
author = "Rajilić-Stojanović, Mirjana and Biagi, Elena and Heilig, Hans G. H. J. and Kajander, Kajsa and Kekkonen, Riina A. and Tims, Sebastian and de Vos, Willem M.",
year = "2011",
abstract = "BACKGROUND & AIMS: Irritable bowel syndrome (IBS) has been associated with disruptions to the intestinal microbiota, but studies have had limited power, coverage, and depth of analysis. We aimed to define microbial populations that can be used discriminate the fecal microbiota of patients with IBS from that of healthy subjects and correlate these with IBS intestinal symptom scores. METHODS: The microbiota composition was assessed by global and deep molecular analysis of fecal samples from 62 patients with IBS patients and 46 healthy individuals (controls). We used a comprehensive and highly reproducible phylogenetic microarray in combination with quantitative polymerase chain reaction. RESULTS: The intestinal microbiota of IBS patients differed significantly (P=.0005) from that of controls. The microbiota of patients, compared with controls, had a 2-fold increased ratio of the Firmicutes to Bacteroidetes (P=.0002). This resulted from an approximately 1.5-fold increase in numbers of Dorea, Ruminococcus, and Clostridium spp (P lt .005); a 2-fold decrease in the number of Bacteroidetes (P lt .0001); a 1.5-fold decrease in numbers of Bifidobacterium and Faecalibacterium spp (P lt .05); and, when present, a 4-fold lower average number of methanogens (3.50 x 10(7) vs 8.74 x 10(6) cells/g feces; P=.003). Correlation analysis of the microbial groups and IBS symptom scores indicated the involvement of several groups of Firmicutes and Proteobacteria in the pathogenesis of IBS. CONCLUSIONS: Global and deep molecular analysis of fecal samples indicates that patients with IBS have a different composition of microbiota. This information might be used to develop better diagnostics and ultimately treatments for IBS.",
publisher = "W B Saunders Co-Elsevier Inc, Philadelphia",
journal = "Gastroenterology",
title = "Global and Deep Molecular Analysis of Microbiota Signatures in Fecal Samples From Patients With Irritable Bowel Syndrome",
pages = "1801-1792",
number = "5",
volume = "141",
doi = "10.1053/j.gastro.2011.07.043",
url = "conv_3686"
}
Rajilić-Stojanović, M., Biagi, E., Heilig, H. G. H. J., Kajander, K., Kekkonen, R. A., Tims, S.,& de Vos, W. M.. (2011). Global and Deep Molecular Analysis of Microbiota Signatures in Fecal Samples From Patients With Irritable Bowel Syndrome. in Gastroenterology
W B Saunders Co-Elsevier Inc, Philadelphia., 141(5), 1792-1801.
https://doi.org/10.1053/j.gastro.2011.07.043
conv_3686
Rajilić-Stojanović M, Biagi E, Heilig HGHJ, Kajander K, Kekkonen RA, Tims S, de Vos WM. Global and Deep Molecular Analysis of Microbiota Signatures in Fecal Samples From Patients With Irritable Bowel Syndrome. in Gastroenterology. 2011;141(5):1792-1801.
doi:10.1053/j.gastro.2011.07.043
conv_3686 .
Rajilić-Stojanović, Mirjana, Biagi, Elena, Heilig, Hans G. H. J., Kajander, Kajsa, Kekkonen, Riina A., Tims, Sebastian, de Vos, Willem M., "Global and Deep Molecular Analysis of Microbiota Signatures in Fecal Samples From Patients With Irritable Bowel Syndrome" in Gastroenterology, 141, no. 5 (2011):1792-1801,
https://doi.org/10.1053/j.gastro.2011.07.043 .,
conv_3686 .

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