Show simple item record

dc.creatorPrlainović, Nevena
dc.creatorKnežević-Jugović, Zorica
dc.creatorMijin, Dušan
dc.creatorBezbradica, Dejan
dc.date.accessioned2021-03-10T11:38:22Z
dc.date.available2021-03-10T11:38:22Z
dc.date.issued2011
dc.identifier.issn1615-7591
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/1939
dc.description.abstractThe objective of this paper was the investigation of a suitable Sepabeads(A (R)) support and method for immobilization of lipase from Candida rugosa. Three different supports were used, two with amino groups, (Sepabeads(A (R)) EC-EA and Sepabeads(A (R)) EC-HA), differing in spacer length (two and six carbons, respectively) and one with epoxy group (Sepabeads(A (R)) EC-EP). Lipase immobilization was carried out by two conventional methods (via epoxy groups and via glutaraldehyde), and with periodate method for modification of lipase. The results of activity assays showed that lipase retained 94.8% or 87.6% of activity after immobilization via epoxy groups or with periodate method, respectively, while glutaraldehyde method was inferior with only 12.7% of retention. The immobilization of lipase, previously modified by periodate oxidation, via amino groups has proven to be more efficient than direct immobilization of lipase via epoxy groups. In such a way immobilized enzyme exhibited higher activity at high reaction temperatures and higher thermal stability.en
dc.publisherSpringer, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172013/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.rightsrestrictedAccess
dc.sourceBioprocess and Biosystems Engineering
dc.subjectEnzyme immobilizationen
dc.subjectCandida rugosa lipaseen
dc.subjectSepabeads (R)en
dc.subjectPeriodate oxidationen
dc.titleImmobilization of lipase from Candida rugosa on Sepabeads(A (R)): the effect of lipase oxidation by periodatesen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage810
dc.citation.issue7
dc.citation.other34(7): 803-810
dc.citation.rankM22
dc.citation.spage803
dc.citation.volume34
dc.identifier.doi10.1007/s00449-011-0530-2
dc.identifier.pmid21347667
dc.identifier.rcubconv_3644
dc.identifier.scopus2-s2.0-81755187222
dc.identifier.wos000293896200004
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record