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One-step, inexpensive high yield strategy for Candida antarctica lipase A isolation using hydroxyapatite
(Elsevier Sci Ltd, Oxford, 2012)
Lipase A from Candida antarctica (CAL A) was purified to apparent homogeneity in a single step using hydroxyapatite (HAP) chromatography. CAL A bound to HAP was eluted with 10 mM Na-phosphate buffer, pH 7.0 containing 0.5% ...