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dc.creatorNastasijević, Branislav J.
dc.creatorLazarević-Pašti, Tamara
dc.creatorDimitrijević-Branković, Suzana
dc.creatorPašti, Igor A.
dc.creatorVujačić, Ana V.
dc.creatorJoksić, Gordana
dc.creatorVasić, Vesna M.
dc.date.accessioned2021-03-10T11:50:27Z
dc.date.available2021-03-10T11:50:27Z
dc.date.issued2012
dc.identifier.issn0731-7085
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/2130
dc.description.abstractThe aim of this study was to investigate the inhibitory activity of Gentiana lutea extracts on the enzyme myeloperoxidase (MPO), as well as the antioxidant activity of these extracts and their correlation with the total polyphenol content. Extracts were prepared using methanol (100%), water and ethanol aqueous solutions (96, 75, 50 and 25% v/v) as solvents for extraction. Also, isovitexin, amarogentin and gentiopicroside, pharmacologically active constituents of G. lutea were tested as potential inhibitors of MPO. Antioxidant activity of extracts was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging test and also using cyclic voltammetry (CV). Among all extracts, the antioxidant capacity of 50% ethanol aqueous extract was the highest, both when measured using the DPPH test, with IC50 = 20.6 mu g/ml, and when using CV. Also, 50% ethanol extract, showed the best inhibition of MPO activity in comparison with other extracts. In the group of the selected G. lutea constituents, gentiopicroside has proved to be the strongest inhibitor of MPO, with IC50 = 0.8 mu g/ml. Also, the concentration of G. lutea constituents were determined in all extracts, using Ultra Performance Liquid Chromatography (UPLC).en
dc.publisherElsevier, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172023/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Pharmaceutical and Biomedical Analysis
dc.subjectCyclic voltammetryen
dc.subjectDPPH testen
dc.subjectGentiana luteaen
dc.subjectGentiopicosideen
dc.subjectMyeloperoxidaseen
dc.subjectUPLCen
dc.titleInhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extractsen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage196
dc.citation.other66: 191-196
dc.citation.rankM21
dc.citation.spage191
dc.citation.volume66
dc.identifier.doi10.1016/j.jpba.2012.03.052
dc.identifier.pmid22521634
dc.identifier.scopus2-s2.0-84861221232
dc.identifier.wos000304843600023
dc.type.versionpublishedVersion


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Приказ основних података о документу