Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions
Само за регистроване кориснике
2012
Аутори
Milašinović, NikolaMilosavljević, Nedeljko
Filipović, Jovanka
Knežević-Jugović, Zorica
Kalagasidis Krušić, Melina
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Temperature and pH-sensitive hydrogels, based on N-isopropylacrylamide and itaconic acid, with varying comonomer ratios and crosslinking agent content, were prepared by free radical crosslinking copolymerization. The immobilization of lipase from Candida rugosa was carried out by post-loading entrapment method at different temperatures until equilibrium swelling was achieved. The effects of the hydrogel composition and the immobilization temperature on the hydrogel-binding capacity, immobilized lipase specific activity, as well as the enzyme leakage were studied. It was found that the NiPAAm/IA ratio, crosslinking agent concentration and the temperature at which the entrapment was performed significantly affected the hydrogel-binding capacity. The biocatalysts obtained by entrapment into hydrogel with the highest itaconic acid content at 5 A degrees C exhibited both highest binding capacity and the highest specific activity, but appeared to be less suitable for repeated uses than those... obtained at 25 A degrees C.
Кључне речи:
Lipase from Candida rugosa / Immobilization temperature / Hydrogel / N-isopropylacrylamide / Itaconic acidИзвор:
Polymer Bulletin, 2012, 69, 3, 347-361Издавач:
- Springer, New York
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Синтеза и карактеризација нових функционалних полимера и полимерних нанокомпозита (RS-MESTD-Basic Research (BR or ON)-172062)
DOI: 10.1007/s00289-012-0737-7
ISSN: 0170-0839
WoS: 000305227500008
Scopus: 2-s2.0-84864616485
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Milašinović, Nikola AU - Milosavljević, Nedeljko AU - Filipović, Jovanka AU - Knežević-Jugović, Zorica AU - Kalagasidis Krušić, Melina PY - 2012 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2132 AB - Temperature and pH-sensitive hydrogels, based on N-isopropylacrylamide and itaconic acid, with varying comonomer ratios and crosslinking agent content, were prepared by free radical crosslinking copolymerization. The immobilization of lipase from Candida rugosa was carried out by post-loading entrapment method at different temperatures until equilibrium swelling was achieved. The effects of the hydrogel composition and the immobilization temperature on the hydrogel-binding capacity, immobilized lipase specific activity, as well as the enzyme leakage were studied. It was found that the NiPAAm/IA ratio, crosslinking agent concentration and the temperature at which the entrapment was performed significantly affected the hydrogel-binding capacity. The biocatalysts obtained by entrapment into hydrogel with the highest itaconic acid content at 5 A degrees C exhibited both highest binding capacity and the highest specific activity, but appeared to be less suitable for repeated uses than those obtained at 25 A degrees C. PB - Springer, New York T2 - Polymer Bulletin T1 - Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions EP - 361 IS - 3 SP - 347 VL - 69 DO - 10.1007/s00289-012-0737-7 ER -
@article{ author = "Milašinović, Nikola and Milosavljević, Nedeljko and Filipović, Jovanka and Knežević-Jugović, Zorica and Kalagasidis Krušić, Melina", year = "2012", abstract = "Temperature and pH-sensitive hydrogels, based on N-isopropylacrylamide and itaconic acid, with varying comonomer ratios and crosslinking agent content, were prepared by free radical crosslinking copolymerization. The immobilization of lipase from Candida rugosa was carried out by post-loading entrapment method at different temperatures until equilibrium swelling was achieved. The effects of the hydrogel composition and the immobilization temperature on the hydrogel-binding capacity, immobilized lipase specific activity, as well as the enzyme leakage were studied. It was found that the NiPAAm/IA ratio, crosslinking agent concentration and the temperature at which the entrapment was performed significantly affected the hydrogel-binding capacity. The biocatalysts obtained by entrapment into hydrogel with the highest itaconic acid content at 5 A degrees C exhibited both highest binding capacity and the highest specific activity, but appeared to be less suitable for repeated uses than those obtained at 25 A degrees C.", publisher = "Springer, New York", journal = "Polymer Bulletin", title = "Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions", pages = "361-347", number = "3", volume = "69", doi = "10.1007/s00289-012-0737-7" }
Milašinović, N., Milosavljević, N., Filipović, J., Knežević-Jugović, Z.,& Kalagasidis Krušić, M.. (2012). Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions. in Polymer Bulletin Springer, New York., 69(3), 347-361. https://doi.org/10.1007/s00289-012-0737-7
Milašinović N, Milosavljević N, Filipović J, Knežević-Jugović Z, Kalagasidis Krušić M. Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions. in Polymer Bulletin. 2012;69(3):347-361. doi:10.1007/s00289-012-0737-7 .
Milašinović, Nikola, Milosavljević, Nedeljko, Filipović, Jovanka, Knežević-Jugović, Zorica, Kalagasidis Krušić, Melina, "Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions" in Polymer Bulletin, 69, no. 3 (2012):347-361, https://doi.org/10.1007/s00289-012-0737-7 . .