Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions
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2012
Authors
Milašinović, NikolaMilosavljević, Nedeljko
Filipović, Jovanka
Knežević-Jugović, Zorica
Kalagasidis Krušić, Melina
Article (Published version)
Metadata
Show full item recordAbstract
Temperature and pH-sensitive hydrogels, based on N-isopropylacrylamide and itaconic acid, with varying comonomer ratios and crosslinking agent content, were prepared by free radical crosslinking copolymerization. The immobilization of lipase from Candida rugosa was carried out by post-loading entrapment method at different temperatures until equilibrium swelling was achieved. The effects of the hydrogel composition and the immobilization temperature on the hydrogel-binding capacity, immobilized lipase specific activity, as well as the enzyme leakage were studied. It was found that the NiPAAm/IA ratio, crosslinking agent concentration and the temperature at which the entrapment was performed significantly affected the hydrogel-binding capacity. The biocatalysts obtained by entrapment into hydrogel with the highest itaconic acid content at 5 A degrees C exhibited both highest binding capacity and the highest specific activity, but appeared to be less suitable for repeated uses than those... obtained at 25 A degrees C.
Keywords:
Lipase from Candida rugosa / Immobilization temperature / Hydrogel / N-isopropylacrylamide / Itaconic acidSource:
Polymer Bulletin, 2012, 69, 3, 347-361Publisher:
- Springer, New York
Funding / projects:
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)
- Synthesis and characterization of novel functional polymers and polymeric nanocomposites (RS-172062)
DOI: 10.1007/s00289-012-0737-7
ISSN: 0170-0839
WoS: 000305227500008
Scopus: 2-s2.0-84864616485
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Milašinović, Nikola AU - Milosavljević, Nedeljko AU - Filipović, Jovanka AU - Knežević-Jugović, Zorica AU - Kalagasidis Krušić, Melina PY - 2012 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2132 AB - Temperature and pH-sensitive hydrogels, based on N-isopropylacrylamide and itaconic acid, with varying comonomer ratios and crosslinking agent content, were prepared by free radical crosslinking copolymerization. The immobilization of lipase from Candida rugosa was carried out by post-loading entrapment method at different temperatures until equilibrium swelling was achieved. The effects of the hydrogel composition and the immobilization temperature on the hydrogel-binding capacity, immobilized lipase specific activity, as well as the enzyme leakage were studied. It was found that the NiPAAm/IA ratio, crosslinking agent concentration and the temperature at which the entrapment was performed significantly affected the hydrogel-binding capacity. The biocatalysts obtained by entrapment into hydrogel with the highest itaconic acid content at 5 A degrees C exhibited both highest binding capacity and the highest specific activity, but appeared to be less suitable for repeated uses than those obtained at 25 A degrees C. PB - Springer, New York T2 - Polymer Bulletin T1 - Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions EP - 361 IS - 3 SP - 347 VL - 69 DO - 10.1007/s00289-012-0737-7 ER -
@article{ author = "Milašinović, Nikola and Milosavljević, Nedeljko and Filipović, Jovanka and Knežević-Jugović, Zorica and Kalagasidis Krušić, Melina", year = "2012", abstract = "Temperature and pH-sensitive hydrogels, based on N-isopropylacrylamide and itaconic acid, with varying comonomer ratios and crosslinking agent content, were prepared by free radical crosslinking copolymerization. The immobilization of lipase from Candida rugosa was carried out by post-loading entrapment method at different temperatures until equilibrium swelling was achieved. The effects of the hydrogel composition and the immobilization temperature on the hydrogel-binding capacity, immobilized lipase specific activity, as well as the enzyme leakage were studied. It was found that the NiPAAm/IA ratio, crosslinking agent concentration and the temperature at which the entrapment was performed significantly affected the hydrogel-binding capacity. The biocatalysts obtained by entrapment into hydrogel with the highest itaconic acid content at 5 A degrees C exhibited both highest binding capacity and the highest specific activity, but appeared to be less suitable for repeated uses than those obtained at 25 A degrees C.", publisher = "Springer, New York", journal = "Polymer Bulletin", title = "Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions", pages = "361-347", number = "3", volume = "69", doi = "10.1007/s00289-012-0737-7" }
Milašinović, N., Milosavljević, N., Filipović, J., Knežević-Jugović, Z.,& Kalagasidis Krušić, M.. (2012). Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions. in Polymer Bulletin Springer, New York., 69(3), 347-361. https://doi.org/10.1007/s00289-012-0737-7
Milašinović N, Milosavljević N, Filipović J, Knežević-Jugović Z, Kalagasidis Krušić M. Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions. in Polymer Bulletin. 2012;69(3):347-361. doi:10.1007/s00289-012-0737-7 .
Milašinović, Nikola, Milosavljević, Nedeljko, Filipović, Jovanka, Knežević-Jugović, Zorica, Kalagasidis Krušić, Melina, "Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions" in Polymer Bulletin, 69, no. 3 (2012):347-361, https://doi.org/10.1007/s00289-012-0737-7 . .