Приказ основних података о документу

dc.creatorMilašinović, Nikola
dc.creatorMilosavljević, Nedeljko
dc.creatorFilipović, Jovanka
dc.creatorKnežević-Jugović, Zorica
dc.creatorKalagasidis Krušić, Melina
dc.date.accessioned2021-03-10T11:50:34Z
dc.date.available2021-03-10T11:50:34Z
dc.date.issued2012
dc.identifier.issn0170-0839
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/2132
dc.description.abstractTemperature and pH-sensitive hydrogels, based on N-isopropylacrylamide and itaconic acid, with varying comonomer ratios and crosslinking agent content, were prepared by free radical crosslinking copolymerization. The immobilization of lipase from Candida rugosa was carried out by post-loading entrapment method at different temperatures until equilibrium swelling was achieved. The effects of the hydrogel composition and the immobilization temperature on the hydrogel-binding capacity, immobilized lipase specific activity, as well as the enzyme leakage were studied. It was found that the NiPAAm/IA ratio, crosslinking agent concentration and the temperature at which the entrapment was performed significantly affected the hydrogel-binding capacity. The biocatalysts obtained by entrapment into hydrogel with the highest itaconic acid content at 5 A degrees C exhibited both highest binding capacity and the highest specific activity, but appeared to be less suitable for repeated uses than those obtained at 25 A degrees C.en
dc.publisherSpringer, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172062/RS//
dc.rightsrestrictedAccess
dc.sourcePolymer Bulletin
dc.subjectLipase from Candida rugosaen
dc.subjectImmobilization temperatureen
dc.subjectHydrogelen
dc.subjectN-isopropylacrylamideen
dc.subjectItaconic aciden
dc.titleEfficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditionsen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage361
dc.citation.issue3
dc.citation.other69(3): 347-361
dc.citation.rankM22
dc.citation.spage347
dc.citation.volume69
dc.identifier.doi10.1007/s00289-012-0737-7
dc.identifier.scopus2-s2.0-84864616485
dc.identifier.wos000305227500008
dc.type.versionpublishedVersion


Документи

Thumbnail

Овај документ се појављује у следећим колекцијама

Приказ основних података о документу