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Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels

Authorized Users Only
2012
Authors
Milašinović, Nikola
Knežević-Jugović, Zorica
Jakovljević, Živana
Filipović, Jovanka
Kalagasidis-Krušić, Melina
Article (Published version)
Metadata
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Abstract
A commercial Candida rugosa lipase was immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogel under different pH immobilization conditions, characterized and evaluated for both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium. Although the apparent enzyme activity in the hydrolytic reaction was acceptable, the immobilized lipase appeared to be more suitable for application in ester synthesis in low aqueous system based on n-hexane. The esterification reaction parameters, such as temperature, biocatalyst amount, added water content and substrate concentration was evaluated and optimized by response surface analysis. An adequate statistical quadratic model was obtained, making it possible to predict ester yields from known values of the four main factors. Under optimized conditions, the yield of ester gt 90% could be achieved after 48 h of reaction, corresponding to the volumetric activity of 4.75 mmol L-1 h(-1). The operational stab...ility of the immobilized system in esterification reaction proved to be highly attractive with 15 consecutive 48 h uses with a residual activity of 67.4%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.

Keywords:
N-Isopropylacrylamide / Itaconic acid / Enzyme immobilization / Candida rugosa lipase / Ester synthesis
Source:
Chemical Engineering Journal, 2012, 181, 614-623
Publisher:
  • Elsevier Science Sa, Lausanne
Funding / projects:
  • Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)
  • Synthesis and characterization of novel functional polymers and polymeric nanocomposites (RS-172062)

DOI: 10.1016/j.cej.2011.11.115

ISSN: 1385-8947

WoS: 000301087000069

Scopus: 2-s2.0-84856318182
[ Google Scholar ]
23
21
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2235
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
Institution/Community
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Milašinović, Nikola
AU  - Knežević-Jugović, Zorica
AU  - Jakovljević, Živana
AU  - Filipović, Jovanka
AU  - Kalagasidis-Krušić, Melina
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2235
AB  - A commercial Candida rugosa lipase was immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogel under different pH immobilization conditions, characterized and evaluated for both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium. Although the apparent enzyme activity in the hydrolytic reaction was acceptable, the immobilized lipase appeared to be more suitable for application in ester synthesis in low aqueous system based on n-hexane. The esterification reaction parameters, such as temperature, biocatalyst amount, added water content and substrate concentration was evaluated and optimized by response surface analysis. An adequate statistical quadratic model was obtained, making it possible to predict ester yields from known values of the four main factors. Under optimized conditions, the yield of ester  gt 90% could be achieved after 48 h of reaction, corresponding to the volumetric activity of 4.75 mmol L-1 h(-1). The operational stability of the immobilized system in esterification reaction proved to be highly attractive with 15 consecutive 48 h uses with a residual activity of 67.4%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.
PB  - Elsevier Science Sa, Lausanne
T2  - Chemical Engineering Journal
T1  - Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels
EP  - 623
SP  - 614
VL  - 181
DO  - 10.1016/j.cej.2011.11.115
UR  - conv_3768
ER  - 
@article{
author = "Milašinović, Nikola and Knežević-Jugović, Zorica and Jakovljević, Živana and Filipović, Jovanka and Kalagasidis-Krušić, Melina",
year = "2012",
abstract = "A commercial Candida rugosa lipase was immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogel under different pH immobilization conditions, characterized and evaluated for both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium. Although the apparent enzyme activity in the hydrolytic reaction was acceptable, the immobilized lipase appeared to be more suitable for application in ester synthesis in low aqueous system based on n-hexane. The esterification reaction parameters, such as temperature, biocatalyst amount, added water content and substrate concentration was evaluated and optimized by response surface analysis. An adequate statistical quadratic model was obtained, making it possible to predict ester yields from known values of the four main factors. Under optimized conditions, the yield of ester  gt 90% could be achieved after 48 h of reaction, corresponding to the volumetric activity of 4.75 mmol L-1 h(-1). The operational stability of the immobilized system in esterification reaction proved to be highly attractive with 15 consecutive 48 h uses with a residual activity of 67.4%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Chemical Engineering Journal",
title = "Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels",
pages = "623-614",
volume = "181",
doi = "10.1016/j.cej.2011.11.115",
url = "conv_3768"
}
Milašinović, N., Knežević-Jugović, Z., Jakovljević, Ž., Filipović, J.,& Kalagasidis-Krušić, M.. (2012). Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels. in Chemical Engineering Journal
Elsevier Science Sa, Lausanne., 181, 614-623.
https://doi.org/10.1016/j.cej.2011.11.115
conv_3768
Milašinović N, Knežević-Jugović Z, Jakovljević Ž, Filipović J, Kalagasidis-Krušić M. Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels. in Chemical Engineering Journal. 2012;181:614-623.
doi:10.1016/j.cej.2011.11.115
conv_3768 .
Milašinović, Nikola, Knežević-Jugović, Zorica, Jakovljević, Živana, Filipović, Jovanka, Kalagasidis-Krušić, Melina, "Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels" in Chemical Engineering Journal, 181 (2012):614-623,
https://doi.org/10.1016/j.cej.2011.11.115 .,
conv_3768 .

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