A study of transglucosylation kinetic in an enzymatic synthesis of benzyl alcohol glucoside by alpha-glucosidase from S-cerevisiae
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2013
Authors
Pavlović, MarijanaDimitrijević, Aleksandra
Trbojević-Ivić, Jovana
Milosavić, Nenad
Gavrović-Jankulović, Marija
Bezbradica, Dejan
Veličković, Dušan
Article (Published version)
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Show full item recordAbstract
alpha-1,4-Glucosidase from Saccharomyces cerevisiae is an enzyme which is widely used in synthesis of different drugs. Glucosidase inhibitors are studied as potential drugs for prevention of HIV and diabetes. For understanding of these processes it is very important to have insights in the transglucosylation activity of this enzyme. In this paper the kinetics of transglucosylation reaction catalyzed by this enzyme in the synthesis of benzyl alcohol glucoside was studied and all relevant kinetic constants for this system are found. It was shown one additional property of transglycosylation reactions catalyzed by glycosidases-inhibition by both, glucose acceptor and glucose donor, and mechanisms for these inhibitions were proposed.
Keywords:
alpha-glucosidase / transglucosylation / benzyl alcohol glucoside / substrate inhibition / ping-pong mechanisamSource:
Russian Journal of Physical Chemistry A, 2013, 87, 13, 2285-2288Publisher:
- Maik Nauka/Interperiodica/Springer, New York
Funding / projects:
- Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research (EU-FP7-256716)
- Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-MESTD-Basic Research (BR or ON)-172049)
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.1134/S0036024413130207
ISSN: 0036-0244
WoS: 000326718200028
Scopus: 2-s2.0-84888146485
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Pavlović, Marijana AU - Dimitrijević, Aleksandra AU - Trbojević-Ivić, Jovana AU - Milosavić, Nenad AU - Gavrović-Jankulović, Marija AU - Bezbradica, Dejan AU - Veličković, Dušan PY - 2013 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2448 AB - alpha-1,4-Glucosidase from Saccharomyces cerevisiae is an enzyme which is widely used in synthesis of different drugs. Glucosidase inhibitors are studied as potential drugs for prevention of HIV and diabetes. For understanding of these processes it is very important to have insights in the transglucosylation activity of this enzyme. In this paper the kinetics of transglucosylation reaction catalyzed by this enzyme in the synthesis of benzyl alcohol glucoside was studied and all relevant kinetic constants for this system are found. It was shown one additional property of transglycosylation reactions catalyzed by glycosidases-inhibition by both, glucose acceptor and glucose donor, and mechanisms for these inhibitions were proposed. PB - Maik Nauka/Interperiodica/Springer, New York T2 - Russian Journal of Physical Chemistry A T1 - A study of transglucosylation kinetic in an enzymatic synthesis of benzyl alcohol glucoside by alpha-glucosidase from S-cerevisiae EP - 2288 IS - 13 SP - 2285 VL - 87 DO - 10.1134/S0036024413130207 ER -
@article{ author = "Pavlović, Marijana and Dimitrijević, Aleksandra and Trbojević-Ivić, Jovana and Milosavić, Nenad and Gavrović-Jankulović, Marija and Bezbradica, Dejan and Veličković, Dušan", year = "2013", abstract = "alpha-1,4-Glucosidase from Saccharomyces cerevisiae is an enzyme which is widely used in synthesis of different drugs. Glucosidase inhibitors are studied as potential drugs for prevention of HIV and diabetes. For understanding of these processes it is very important to have insights in the transglucosylation activity of this enzyme. In this paper the kinetics of transglucosylation reaction catalyzed by this enzyme in the synthesis of benzyl alcohol glucoside was studied and all relevant kinetic constants for this system are found. It was shown one additional property of transglycosylation reactions catalyzed by glycosidases-inhibition by both, glucose acceptor and glucose donor, and mechanisms for these inhibitions were proposed.", publisher = "Maik Nauka/Interperiodica/Springer, New York", journal = "Russian Journal of Physical Chemistry A", title = "A study of transglucosylation kinetic in an enzymatic synthesis of benzyl alcohol glucoside by alpha-glucosidase from S-cerevisiae", pages = "2288-2285", number = "13", volume = "87", doi = "10.1134/S0036024413130207" }
Pavlović, M., Dimitrijević, A., Trbojević-Ivić, J., Milosavić, N., Gavrović-Jankulović, M., Bezbradica, D.,& Veličković, D.. (2013). A study of transglucosylation kinetic in an enzymatic synthesis of benzyl alcohol glucoside by alpha-glucosidase from S-cerevisiae. in Russian Journal of Physical Chemistry A Maik Nauka/Interperiodica/Springer, New York., 87(13), 2285-2288. https://doi.org/10.1134/S0036024413130207
Pavlović M, Dimitrijević A, Trbojević-Ivić J, Milosavić N, Gavrović-Jankulović M, Bezbradica D, Veličković D. A study of transglucosylation kinetic in an enzymatic synthesis of benzyl alcohol glucoside by alpha-glucosidase from S-cerevisiae. in Russian Journal of Physical Chemistry A. 2013;87(13):2285-2288. doi:10.1134/S0036024413130207 .
Pavlović, Marijana, Dimitrijević, Aleksandra, Trbojević-Ivić, Jovana, Milosavić, Nenad, Gavrović-Jankulović, Marija, Bezbradica, Dejan, Veličković, Dušan, "A study of transglucosylation kinetic in an enzymatic synthesis of benzyl alcohol glucoside by alpha-glucosidase from S-cerevisiae" in Russian Journal of Physical Chemistry A, 87, no. 13 (2013):2285-2288, https://doi.org/10.1134/S0036024413130207 . .