Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes
Само за регистроване кориснике
2013
Аутори
Prlainović, NevenaBezbradica, Dejan
Knežević-Jugović, Zorica
Stevanović, Sanja
Avramov-Ivić, Milka
Uskoković, Petar
Mijin, Dušan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.
Кључне речи:
Lipase / Carbon nanotubes / Adsorption / FT-IR spectroscopy / AFM / Cyclic voltammetryИзвор:
Journal of Industrial and Engineering Chemistry, 2013, 19, 1, 279-285Издавач:
- Elsevier Science Inc, New York
Финансирање / пројекти:
- Проучавање синтезе, структуре и активности органских једињења природног и синтетског порекла (RS-172013)
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-46010)
- Синтеза, развој технологија добијања и примена наноструктурних мултифункционалних материјала дефинисаних својстава (RS-45019)
DOI: 10.1016/j.jiec.2012.08.012
ISSN: 1226-086X
WoS: 000312764700039
Scopus: 2-s2.0-84870399209
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Prlainović, Nevena AU - Bezbradica, Dejan AU - Knežević-Jugović, Zorica AU - Stevanović, Sanja AU - Avramov-Ivić, Milka AU - Uskoković, Petar AU - Mijin, Dušan PY - 2013 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2477 AB - In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry. PB - Elsevier Science Inc, New York T2 - Journal of Industrial and Engineering Chemistry T1 - Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes EP - 285 IS - 1 SP - 279 VL - 19 DO - 10.1016/j.jiec.2012.08.012 ER -
@article{ author = "Prlainović, Nevena and Bezbradica, Dejan and Knežević-Jugović, Zorica and Stevanović, Sanja and Avramov-Ivić, Milka and Uskoković, Petar and Mijin, Dušan", year = "2013", abstract = "In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.", publisher = "Elsevier Science Inc, New York", journal = "Journal of Industrial and Engineering Chemistry", title = "Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes", pages = "285-279", number = "1", volume = "19", doi = "10.1016/j.jiec.2012.08.012" }
Prlainović, N., Bezbradica, D., Knežević-Jugović, Z., Stevanović, S., Avramov-Ivić, M., Uskoković, P.,& Mijin, D.. (2013). Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes. in Journal of Industrial and Engineering Chemistry Elsevier Science Inc, New York., 19(1), 279-285. https://doi.org/10.1016/j.jiec.2012.08.012
Prlainović N, Bezbradica D, Knežević-Jugović Z, Stevanović S, Avramov-Ivić M, Uskoković P, Mijin D. Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes. in Journal of Industrial and Engineering Chemistry. 2013;19(1):279-285. doi:10.1016/j.jiec.2012.08.012 .
Prlainović, Nevena, Bezbradica, Dejan, Knežević-Jugović, Zorica, Stevanović, Sanja, Avramov-Ivić, Milka, Uskoković, Petar, Mijin, Dušan, "Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes" in Journal of Industrial and Engineering Chemistry, 19, no. 1 (2013):279-285, https://doi.org/10.1016/j.jiec.2012.08.012 . .