A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels
Само за регистроване кориснике
2014
Аутори
Stojkovska, JasminaKostić, Danijela
Jovanović, Zeljka
Vukašinović-Sekulić, Maja
Mišković-Stanković, Vesna
Obradović, Bojana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this work, we present a comprehensive approach to evaluation of alginate microbeads with included silver nanoparticles (AgNPs) at the concentration range of 0.3-5 mM for potential biomedical use by combining cytotoxicity, antibacterial activity, and silver release studies. The microbeads were investigated regarding drying and rehydration showing retention of similar to 80-85% of the initial nanoparticles as determined by UV-vis and SEM analyses. Both wet and dry microbeads were shown to release AgNPs and/or ions inducing similar growth delays of Staphylococcus aureus and Escherichia coli at the total released silver concentrations of similar to 10 mu g/ml. On the other hand, these concentrations were highly toxic for bovine chondrocytes in conventional monolayer cultures while nontoxic when cultured in alginate microbeads under biomimetic conditions in 3D perfusion bioreactors. The applied approach outlined directions for further optimization studies demonstrating Ag/alginate microb...eads as potentially attractive components of soft tissue implants as well as antimicrobial wound dressings.
Кључне речи:
Silver nanoparticles / Alginate / Biomimetic bioreactor / Silver release / Cytotoxicity / Antibacterial activityИзвор:
Carbohydrate Polymers, 2014, 111, 305-314Издавач:
- Elsevier Sci Ltd, Oxford
Финансирање / пројекти:
- Синтеза, развој технологија добијања и примена наноструктурних мултифункционалних материјала дефинисаних својстава (RS-45019)
- Eureka E!6749
DOI: 10.1016/j.carbpol.2014.04.063
ISSN: 0144-8617
PubMed: 25037356
WoS: 000340302100035
Scopus: 2-s2.0-84901438318
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Stojkovska, Jasmina AU - Kostić, Danijela AU - Jovanović, Zeljka AU - Vukašinović-Sekulić, Maja AU - Mišković-Stanković, Vesna AU - Obradović, Bojana PY - 2014 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2650 AB - In this work, we present a comprehensive approach to evaluation of alginate microbeads with included silver nanoparticles (AgNPs) at the concentration range of 0.3-5 mM for potential biomedical use by combining cytotoxicity, antibacterial activity, and silver release studies. The microbeads were investigated regarding drying and rehydration showing retention of similar to 80-85% of the initial nanoparticles as determined by UV-vis and SEM analyses. Both wet and dry microbeads were shown to release AgNPs and/or ions inducing similar growth delays of Staphylococcus aureus and Escherichia coli at the total released silver concentrations of similar to 10 mu g/ml. On the other hand, these concentrations were highly toxic for bovine chondrocytes in conventional monolayer cultures while nontoxic when cultured in alginate microbeads under biomimetic conditions in 3D perfusion bioreactors. The applied approach outlined directions for further optimization studies demonstrating Ag/alginate microbeads as potentially attractive components of soft tissue implants as well as antimicrobial wound dressings. PB - Elsevier Sci Ltd, Oxford T2 - Carbohydrate Polymers T1 - A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels EP - 314 SP - 305 VL - 111 DO - 10.1016/j.carbpol.2014.04.063 ER -
@article{ author = "Stojkovska, Jasmina and Kostić, Danijela and Jovanović, Zeljka and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna and Obradović, Bojana", year = "2014", abstract = "In this work, we present a comprehensive approach to evaluation of alginate microbeads with included silver nanoparticles (AgNPs) at the concentration range of 0.3-5 mM for potential biomedical use by combining cytotoxicity, antibacterial activity, and silver release studies. The microbeads were investigated regarding drying and rehydration showing retention of similar to 80-85% of the initial nanoparticles as determined by UV-vis and SEM analyses. Both wet and dry microbeads were shown to release AgNPs and/or ions inducing similar growth delays of Staphylococcus aureus and Escherichia coli at the total released silver concentrations of similar to 10 mu g/ml. On the other hand, these concentrations were highly toxic for bovine chondrocytes in conventional monolayer cultures while nontoxic when cultured in alginate microbeads under biomimetic conditions in 3D perfusion bioreactors. The applied approach outlined directions for further optimization studies demonstrating Ag/alginate microbeads as potentially attractive components of soft tissue implants as well as antimicrobial wound dressings.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Carbohydrate Polymers", title = "A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels", pages = "314-305", volume = "111", doi = "10.1016/j.carbpol.2014.04.063" }
Stojkovska, J., Kostić, D., Jovanović, Z., Vukašinović-Sekulić, M., Mišković-Stanković, V.,& Obradović, B.. (2014). A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels. in Carbohydrate Polymers Elsevier Sci Ltd, Oxford., 111, 305-314. https://doi.org/10.1016/j.carbpol.2014.04.063
Stojkovska J, Kostić D, Jovanović Z, Vukašinović-Sekulić M, Mišković-Stanković V, Obradović B. A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels. in Carbohydrate Polymers. 2014;111:305-314. doi:10.1016/j.carbpol.2014.04.063 .
Stojkovska, Jasmina, Kostić, Danijela, Jovanović, Zeljka, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, Obradović, Bojana, "A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels" in Carbohydrate Polymers, 111 (2014):305-314, https://doi.org/10.1016/j.carbpol.2014.04.063 . .