Lipase-Catalyzed Esterification of Phloridzin: Acyl Donor Effect on Enzymatic Affinity and Antioxidant Properties of Esters
Samo za registrovane korisnike
2014
Autori
Milivojević, AnaStojanović, Marija
Carević, Milica
Mihailović, Mladen
Veličković, Dušan
Milosavić, Nenad
Bezbradica, Dejan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Lipase-catalyzed acylation of phloridzin with a wide range of fatty acids and antioxidant activities of synthesized esters were investigated in this study. Polar solvents were suitable reaction media for esterification, and the highest yield was achieved in acetonitrile. By using statistically designed optimization of key experimental factors for the synthesis of phloridzil oleate as the model reaction, great improvements in achieved conversion and yield per enzyme mass were enabled. The most significant progress has been made in the cost-effectiveness of the reaction, since the specific yield of 5.45 mmol g(-1) was obtained at 58 degrees C, with 0.09 M phloridzin, 1.17 M oleic acid, and only 0.5% (w/v) biocatalyst. All examined fatty acids were suitable acyl donors, since the chain length and unsaturation degree had slight effects on esterification and all products yielded over 70%. Phloridzil caprate, myristate, and oleate exhibited the highest antioxidant activities among the obtain...ed esters.
Izvor:
Industrial & Engineering Chemistry Research, 2014, 53, 43, 16644-16651Izdavač:
- Amer Chemical Soc, Washington
Finansiranje / projekti:
- Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.1021/ie5027259
ISSN: 0888-5885
WoS: 000344042700006
Scopus: 2-s2.0-84908565425
Kolekcije
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Milivojević, Ana AU - Stojanović, Marija AU - Carević, Milica AU - Mihailović, Mladen AU - Veličković, Dušan AU - Milosavić, Nenad AU - Bezbradica, Dejan PY - 2014 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2744 AB - Lipase-catalyzed acylation of phloridzin with a wide range of fatty acids and antioxidant activities of synthesized esters were investigated in this study. Polar solvents were suitable reaction media for esterification, and the highest yield was achieved in acetonitrile. By using statistically designed optimization of key experimental factors for the synthesis of phloridzil oleate as the model reaction, great improvements in achieved conversion and yield per enzyme mass were enabled. The most significant progress has been made in the cost-effectiveness of the reaction, since the specific yield of 5.45 mmol g(-1) was obtained at 58 degrees C, with 0.09 M phloridzin, 1.17 M oleic acid, and only 0.5% (w/v) biocatalyst. All examined fatty acids were suitable acyl donors, since the chain length and unsaturation degree had slight effects on esterification and all products yielded over 70%. Phloridzil caprate, myristate, and oleate exhibited the highest antioxidant activities among the obtained esters. PB - Amer Chemical Soc, Washington T2 - Industrial & Engineering Chemistry Research T1 - Lipase-Catalyzed Esterification of Phloridzin: Acyl Donor Effect on Enzymatic Affinity and Antioxidant Properties of Esters EP - 16651 IS - 43 SP - 16644 VL - 53 DO - 10.1021/ie5027259 ER -
@article{ author = "Milivojević, Ana and Stojanović, Marija and Carević, Milica and Mihailović, Mladen and Veličković, Dušan and Milosavić, Nenad and Bezbradica, Dejan", year = "2014", abstract = "Lipase-catalyzed acylation of phloridzin with a wide range of fatty acids and antioxidant activities of synthesized esters were investigated in this study. Polar solvents were suitable reaction media for esterification, and the highest yield was achieved in acetonitrile. By using statistically designed optimization of key experimental factors for the synthesis of phloridzil oleate as the model reaction, great improvements in achieved conversion and yield per enzyme mass were enabled. The most significant progress has been made in the cost-effectiveness of the reaction, since the specific yield of 5.45 mmol g(-1) was obtained at 58 degrees C, with 0.09 M phloridzin, 1.17 M oleic acid, and only 0.5% (w/v) biocatalyst. All examined fatty acids were suitable acyl donors, since the chain length and unsaturation degree had slight effects on esterification and all products yielded over 70%. Phloridzil caprate, myristate, and oleate exhibited the highest antioxidant activities among the obtained esters.", publisher = "Amer Chemical Soc, Washington", journal = "Industrial & Engineering Chemistry Research", title = "Lipase-Catalyzed Esterification of Phloridzin: Acyl Donor Effect on Enzymatic Affinity and Antioxidant Properties of Esters", pages = "16651-16644", number = "43", volume = "53", doi = "10.1021/ie5027259" }
Milivojević, A., Stojanović, M., Carević, M., Mihailović, M., Veličković, D., Milosavić, N.,& Bezbradica, D.. (2014). Lipase-Catalyzed Esterification of Phloridzin: Acyl Donor Effect on Enzymatic Affinity and Antioxidant Properties of Esters. in Industrial & Engineering Chemistry Research Amer Chemical Soc, Washington., 53(43), 16644-16651. https://doi.org/10.1021/ie5027259
Milivojević A, Stojanović M, Carević M, Mihailović M, Veličković D, Milosavić N, Bezbradica D. Lipase-Catalyzed Esterification of Phloridzin: Acyl Donor Effect on Enzymatic Affinity and Antioxidant Properties of Esters. in Industrial & Engineering Chemistry Research. 2014;53(43):16644-16651. doi:10.1021/ie5027259 .
Milivojević, Ana, Stojanović, Marija, Carević, Milica, Mihailović, Mladen, Veličković, Dušan, Milosavić, Nenad, Bezbradica, Dejan, "Lipase-Catalyzed Esterification of Phloridzin: Acyl Donor Effect on Enzymatic Affinity and Antioxidant Properties of Esters" in Industrial & Engineering Chemistry Research, 53, no. 43 (2014):16644-16651, https://doi.org/10.1021/ie5027259 . .