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dc.creatorČolić, Miodrag
dc.creatorDzopalić, Tanja
dc.creatorTomić, Sergej
dc.creatorRajković, Jelena
dc.creatorRudolf, Rebeka
dc.creatorVuković, Goran D.
dc.creatorMarinković, Aleksandar
dc.creatorUskoković, Petar
dc.date.accessioned2021-03-10T12:31:45Z
dc.date.available2021-03-10T12:31:45Z
dc.date.issued2014
dc.identifier.issn0008-6223
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/2782
dc.description.abstractThe protocols for the preparation of dendritic cells (DCs) as cancer vaccines based on stimulation of Toll-like receptors (TLRs) are very promising. In this study we covalently attached 7-thia-8-oxoguanosine (7-TOG), a selective endosomal TLR7, to previously oxidized multi-walled carbon nanotubes (o-MWCNTs) (mass ratio 1:5.6, respectively), and tested their ability to activate human monocyte-derived (Mo)DCs. Light, confocal and transmission electron microscopy confirmed efficient phagocytosis of 7-TOG-MWCNTs by MoDCs and their efficient delivery to TLR7(+) endosomes. The biocompatibility studies showed that 7-TOG-MWCNTs, at concentrations lower than 100 mu g/ml, were not cytotoxic for MoDCs. 7-TOG-MV/CNTs (50 mu g/ml) up-regulated CD86 expression, allostimulatory activity, T helper (Th)1- and Th17-polarizing capability of MoDCs. The same concentration of soluble 7-TOG, alone or in combination with control o-MWCNTs, did not have such effects. It can be hypothesized that the efficacy of 7-TOG-MWCNTs in stimulating MoDCs was a consequence of increased intracellular concentration of 7-TOG after internalization of the nano-complexes, because similar Th-polarizing capability could be induced with 10-times higher concentrations of soluble 7-TOG. In conclusion, our results suggest that functionalized MWCNTs may be a promising system for the delivery of drugs to intracellular targets, in order to improve the immunogenic potential of DCs for therapeutic purposes.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175102/RS//
dc.relationMilitary Medical Academy, Belgrade [MFVMA/8/12-14]
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/245916/EU//
dc.rightsrestrictedAccess
dc.sourceCarbon
dc.titleImmunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cellsen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage287
dc.citation.other67: 273-287
dc.citation.rankaM21
dc.citation.spage273
dc.citation.volume67
dc.identifier.doi10.1016/j.carbon.2013.09.090
dc.identifier.scopus2-s2.0-84892785218
dc.identifier.wos000329379300030
dc.type.versionpublishedVersion


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