Antimicrobial activity and biocompatibility of Ag--(+) and Cu2+ -doped biphasic hydroxyapatite/alpha-tricalcium phosphate obtained from hydrothermally synthesized Ag+- and Cu2+ -doped hydroxyapatite
Samo za registrovane korisnike
2014
Autori
Radovanović, ŽeljkoJokić, Bojan
Veljović, Đorđe
Dimitrijević, Suzana
Kojić, Vesna
Petrović, Rada
Janaćković, Đorđe
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Hydroxyapatite (HAp) powders doped with Ag+ or Cu2+ were synthesized by a hydrothermal method in order to obtain biomaterial with an antimicrobial effect. The synthesis was performed with two contents of dopant (Ag+ or Cu2+) by considering both the antimicrobial activities and biocompatibility of the powders. The doped HAp was annealed at 1200 C for 2 h with the intention of investigating the influence of doping with Ag+ and Cu2+ on the creation of the biphasic HAp/alpha-tricalcium phosphate (HAp/a-TCP) and determining the antimicrobial activity and biocompatibility of the obtained biphasic powders. Analyses of all powders, undoped and doped HAp and HAp/a-TCP, were performed by Field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), atomic absorption spectroscopy (AAS) and energydispersive X-ray spectroscopy (EDS). The in vitro antibacterial activities of the powders were evaluated against: Staphylococcus aureus, Escherichia co li, Pseudomonas aeruginosa and Candi...da albi cans. All powders showed good antimicrobial activity but generally the powders of doped HAp/a-TCP had more uniform results against all pathogenic microorganisms than the powders of doped HAp. In vitro biocompatibility tests, MU and DET, were used to evaluate the biocompatibility of Ag+- and Cu2+-doped HAp/a-TCP with MRC-5 human fibroblast cells. These tests confirmed that powders do not have a cytotoxic effect. The HAp/a-TCP powders doped with the lower content of Ag+ and Cu2+ showed especially good biocompatibility. Antimicrobial and biocompatibility tests recommend the Ag+- and Cu2+-doped HAp/a-TCP as promising material for use in reconstructive surgery of bone.
Ključne reči:
HAp/alpha-TCP / Ag+-doped / Cu2+-doped / Antimicrobial / BiocompatibilityIzvor:
Applied Surface Science, 2014, 307, 513-519Izdavač:
- Elsevier Science Bv, Amsterdam
Finansiranje / projekti:
DOI: 10.1016/j.apsusc.2014.04.066
ISSN: 0169-4332
WoS: 000336596700071
Scopus: 2-s2.0-84901326629
Kolekcije
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Radovanović, Željko AU - Jokić, Bojan AU - Veljović, Đorđe AU - Dimitrijević, Suzana AU - Kojić, Vesna AU - Petrović, Rada AU - Janaćković, Đorđe PY - 2014 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2821 AB - Hydroxyapatite (HAp) powders doped with Ag+ or Cu2+ were synthesized by a hydrothermal method in order to obtain biomaterial with an antimicrobial effect. The synthesis was performed with two contents of dopant (Ag+ or Cu2+) by considering both the antimicrobial activities and biocompatibility of the powders. The doped HAp was annealed at 1200 C for 2 h with the intention of investigating the influence of doping with Ag+ and Cu2+ on the creation of the biphasic HAp/alpha-tricalcium phosphate (HAp/a-TCP) and determining the antimicrobial activity and biocompatibility of the obtained biphasic powders. Analyses of all powders, undoped and doped HAp and HAp/a-TCP, were performed by Field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), atomic absorption spectroscopy (AAS) and energydispersive X-ray spectroscopy (EDS). The in vitro antibacterial activities of the powders were evaluated against: Staphylococcus aureus, Escherichia co li, Pseudomonas aeruginosa and Candida albi cans. All powders showed good antimicrobial activity but generally the powders of doped HAp/a-TCP had more uniform results against all pathogenic microorganisms than the powders of doped HAp. In vitro biocompatibility tests, MU and DET, were used to evaluate the biocompatibility of Ag+- and Cu2+-doped HAp/a-TCP with MRC-5 human fibroblast cells. These tests confirmed that powders do not have a cytotoxic effect. The HAp/a-TCP powders doped with the lower content of Ag+ and Cu2+ showed especially good biocompatibility. Antimicrobial and biocompatibility tests recommend the Ag+- and Cu2+-doped HAp/a-TCP as promising material for use in reconstructive surgery of bone. PB - Elsevier Science Bv, Amsterdam T2 - Applied Surface Science T1 - Antimicrobial activity and biocompatibility of Ag--(+) and Cu2+ -doped biphasic hydroxyapatite/alpha-tricalcium phosphate obtained from hydrothermally synthesized Ag+- and Cu2+ -doped hydroxyapatite EP - 519 SP - 513 VL - 307 DO - 10.1016/j.apsusc.2014.04.066 ER -
@article{ author = "Radovanović, Željko and Jokić, Bojan and Veljović, Đorđe and Dimitrijević, Suzana and Kojić, Vesna and Petrović, Rada and Janaćković, Đorđe", year = "2014", abstract = "Hydroxyapatite (HAp) powders doped with Ag+ or Cu2+ were synthesized by a hydrothermal method in order to obtain biomaterial with an antimicrobial effect. The synthesis was performed with two contents of dopant (Ag+ or Cu2+) by considering both the antimicrobial activities and biocompatibility of the powders. The doped HAp was annealed at 1200 C for 2 h with the intention of investigating the influence of doping with Ag+ and Cu2+ on the creation of the biphasic HAp/alpha-tricalcium phosphate (HAp/a-TCP) and determining the antimicrobial activity and biocompatibility of the obtained biphasic powders. Analyses of all powders, undoped and doped HAp and HAp/a-TCP, were performed by Field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), atomic absorption spectroscopy (AAS) and energydispersive X-ray spectroscopy (EDS). The in vitro antibacterial activities of the powders were evaluated against: Staphylococcus aureus, Escherichia co li, Pseudomonas aeruginosa and Candida albi cans. All powders showed good antimicrobial activity but generally the powders of doped HAp/a-TCP had more uniform results against all pathogenic microorganisms than the powders of doped HAp. In vitro biocompatibility tests, MU and DET, were used to evaluate the biocompatibility of Ag+- and Cu2+-doped HAp/a-TCP with MRC-5 human fibroblast cells. These tests confirmed that powders do not have a cytotoxic effect. The HAp/a-TCP powders doped with the lower content of Ag+ and Cu2+ showed especially good biocompatibility. Antimicrobial and biocompatibility tests recommend the Ag+- and Cu2+-doped HAp/a-TCP as promising material for use in reconstructive surgery of bone.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Applied Surface Science", title = "Antimicrobial activity and biocompatibility of Ag--(+) and Cu2+ -doped biphasic hydroxyapatite/alpha-tricalcium phosphate obtained from hydrothermally synthesized Ag+- and Cu2+ -doped hydroxyapatite", pages = "519-513", volume = "307", doi = "10.1016/j.apsusc.2014.04.066" }
Radovanović, Ž., Jokić, B., Veljović, Đ., Dimitrijević, S., Kojić, V., Petrović, R.,& Janaćković, Đ.. (2014). Antimicrobial activity and biocompatibility of Ag--(+) and Cu2+ -doped biphasic hydroxyapatite/alpha-tricalcium phosphate obtained from hydrothermally synthesized Ag+- and Cu2+ -doped hydroxyapatite. in Applied Surface Science Elsevier Science Bv, Amsterdam., 307, 513-519. https://doi.org/10.1016/j.apsusc.2014.04.066
Radovanović Ž, Jokić B, Veljović Đ, Dimitrijević S, Kojić V, Petrović R, Janaćković Đ. Antimicrobial activity and biocompatibility of Ag--(+) and Cu2+ -doped biphasic hydroxyapatite/alpha-tricalcium phosphate obtained from hydrothermally synthesized Ag+- and Cu2+ -doped hydroxyapatite. in Applied Surface Science. 2014;307:513-519. doi:10.1016/j.apsusc.2014.04.066 .
Radovanović, Željko, Jokić, Bojan, Veljović, Đorđe, Dimitrijević, Suzana, Kojić, Vesna, Petrović, Rada, Janaćković, Đorđe, "Antimicrobial activity and biocompatibility of Ag--(+) and Cu2+ -doped biphasic hydroxyapatite/alpha-tricalcium phosphate obtained from hydrothermally synthesized Ag+- and Cu2+ -doped hydroxyapatite" in Applied Surface Science, 307 (2014):513-519, https://doi.org/10.1016/j.apsusc.2014.04.066 . .