Cytotoxicity studies of Ag/alginate nanocomposite hydrogels in 2D and 3D cultures
Апстракт
Alginate nanocomposite hydrogels with incorporated electrochemically synthesized silver nanoparticles were investigated regarding cytotoxicity in vitro. Direct contact test of Ag/alginate discs was applied in 2D monolayer cultures of bovine calf chondrocytes while a 3D culture of bovine articular cartilage explants pressed by the discs was established in a biomimetic bioreactor with dynamic compression in the physiological regime (10 % strain, 0.84 Hz frequency, 1 h on / 1 h off). Moderate cytotoxicity was observed in 2D cell cultures as opposed to findings in 3D explant cultures, which were not affected by the Ag/alginate discs despite the compression.
Извор:
2015 IEEE 15th International Conference on Bioinformatics and Bioengineering, BIBE 2015, 2015Издавач:
- 15th IEEE International Conference on Bioinformatics and Bioengineering, BIBE 2015
DOI: 10.1109/BIBE.2015.7367664
ISBN: 978-146737983-0
PubMed:
Scopus: 2-s2.0-84962875856
Институција/група
Tehnološko-metalurški fakultetTY - CONF AU - Zvicer, Jovana AU - Samardžić, M. AU - Mišković-Stanković, Vesna AU - Obradović, Bojana PY - 2015 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2880 AB - Alginate nanocomposite hydrogels with incorporated electrochemically synthesized silver nanoparticles were investigated regarding cytotoxicity in vitro. Direct contact test of Ag/alginate discs was applied in 2D monolayer cultures of bovine calf chondrocytes while a 3D culture of bovine articular cartilage explants pressed by the discs was established in a biomimetic bioreactor with dynamic compression in the physiological regime (10 % strain, 0.84 Hz frequency, 1 h on / 1 h off). Moderate cytotoxicity was observed in 2D cell cultures as opposed to findings in 3D explant cultures, which were not affected by the Ag/alginate discs despite the compression. PB - 15th IEEE International Conference on Bioinformatics and Bioengineering, BIBE 2015 C3 - 2015 IEEE 15th International Conference on Bioinformatics and Bioengineering, BIBE 2015 T1 - Cytotoxicity studies of Ag/alginate nanocomposite hydrogels in 2D and 3D cultures DO - 10.1109/BIBE.2015.7367664 ER -
@conference{ author = "Zvicer, Jovana and Samardžić, M. and Mišković-Stanković, Vesna and Obradović, Bojana", year = "2015", abstract = "Alginate nanocomposite hydrogels with incorporated electrochemically synthesized silver nanoparticles were investigated regarding cytotoxicity in vitro. Direct contact test of Ag/alginate discs was applied in 2D monolayer cultures of bovine calf chondrocytes while a 3D culture of bovine articular cartilage explants pressed by the discs was established in a biomimetic bioreactor with dynamic compression in the physiological regime (10 % strain, 0.84 Hz frequency, 1 h on / 1 h off). Moderate cytotoxicity was observed in 2D cell cultures as opposed to findings in 3D explant cultures, which were not affected by the Ag/alginate discs despite the compression.", publisher = "15th IEEE International Conference on Bioinformatics and Bioengineering, BIBE 2015", journal = "2015 IEEE 15th International Conference on Bioinformatics and Bioengineering, BIBE 2015", title = "Cytotoxicity studies of Ag/alginate nanocomposite hydrogels in 2D and 3D cultures", doi = "10.1109/BIBE.2015.7367664" }
Zvicer, J., Samardžić, M., Mišković-Stanković, V.,& Obradović, B.. (2015). Cytotoxicity studies of Ag/alginate nanocomposite hydrogels in 2D and 3D cultures. in 2015 IEEE 15th International Conference on Bioinformatics and Bioengineering, BIBE 2015 15th IEEE International Conference on Bioinformatics and Bioengineering, BIBE 2015.. https://doi.org/10.1109/BIBE.2015.7367664
Zvicer J, Samardžić M, Mišković-Stanković V, Obradović B. Cytotoxicity studies of Ag/alginate nanocomposite hydrogels in 2D and 3D cultures. in 2015 IEEE 15th International Conference on Bioinformatics and Bioengineering, BIBE 2015. 2015;. doi:10.1109/BIBE.2015.7367664 .
Zvicer, Jovana, Samardžić, M., Mišković-Stanković, Vesna, Obradović, Bojana, "Cytotoxicity studies of Ag/alginate nanocomposite hydrogels in 2D and 3D cultures" in 2015 IEEE 15th International Conference on Bioinformatics and Bioengineering, BIBE 2015 (2015), https://doi.org/10.1109/BIBE.2015.7367664 . .