Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery
Određivanje osmotskih osobina životinjskih eritrocita protočnom citometrijom u cilju njihovog inženjeringa kao nosača lekova
2015
Authors
Kostić, Ivana T.Ilić, Vesna Lj.
Bukara, Katarina
Mojsilović, Slavko B.
Đurić, Zorka Ž.
Draškovič, Petra
Bugarski, Branko
Article (Published version)
Metadata
Show full item recordAbstract
Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bov...ine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected.
Uprkos činjenici da su metode koje se baziraju na osmotskim osobinama ćelija najčešće korišćene metode za inkapsulaciju lekova u humane i životinjske eritrocite, podaci o osmotskim osobinama eritrocita životinjskog porekla su vrlo oskudni. Cilj ovog rada bio je ispitivanje mogućnosti korišćenja metode protočne citometrije za određivanje osmotskih osobina svinjskih i goveđih eritrocita, čime bi se olakšao inženjering pomenutih životinjskih eritrocita za otpuštanje lekova. Metodom protočne citometrije uspešno su dobijene informacije o osmotskoj fragilnosti svinjskih i goveđih eritrocita i načinjeni su početni koraci u proceni oblika velikog broja eritrocita. Iako ova metoda nije uspela da potvrdi bubrenje svinjskih eritrocita, ukazala je na razliku u uzorcima svinjskih eritrocita koji su imali osnovne hematološke parametre izvan i unutar referentnih vrednosti. U cilju primene svinjskih i goveđih eritrocita kao nosača lekova, metoda protočne citometrije je, potvrdivši prisustvo osmotski r...azličitih frakcija eritrocita, ukazala na to da se različite količine inkapsuliranog leka u pojedinačnim, kako svinjskim, tako i goveđim eritrocitima mogu očekivati.
Keywords:
flow cytometry / osmotic swelling / osmotic fragility / mechanical fragility / microcytic anemia / erythrocytes / protočna citometrija / osmotsko bubrenje / osmotska fragilnost / mehanička fragilnost / mikrocitna anemija / eritrocitiSource:
Hemijska industrija, 2015, 69, 1, 67-76Publisher:
- Association of Chemical Engineers of Serbia
Funding / projects:
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)
- Ministry of Education, Science and Sport, Republic of Slovenia
DOI: 10.2298/HEMIND140124021K
ISSN: 0367-598X
WoS: 000350913100009
Scopus: 2-s2.0-84923652588
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Kostić, Ivana T. AU - Ilić, Vesna Lj. AU - Bukara, Katarina AU - Mojsilović, Slavko B. AU - Đurić, Zorka Ž. AU - Draškovič, Petra AU - Bugarski, Branko PY - 2015 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2900 AB - Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bovine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected. AB - Uprkos činjenici da su metode koje se baziraju na osmotskim osobinama ćelija najčešće korišćene metode za inkapsulaciju lekova u humane i životinjske eritrocite, podaci o osmotskim osobinama eritrocita životinjskog porekla su vrlo oskudni. Cilj ovog rada bio je ispitivanje mogućnosti korišćenja metode protočne citometrije za određivanje osmotskih osobina svinjskih i goveđih eritrocita, čime bi se olakšao inženjering pomenutih životinjskih eritrocita za otpuštanje lekova. Metodom protočne citometrije uspešno su dobijene informacije o osmotskoj fragilnosti svinjskih i goveđih eritrocita i načinjeni su početni koraci u proceni oblika velikog broja eritrocita. Iako ova metoda nije uspela da potvrdi bubrenje svinjskih eritrocita, ukazala je na razliku u uzorcima svinjskih eritrocita koji su imali osnovne hematološke parametre izvan i unutar referentnih vrednosti. U cilju primene svinjskih i goveđih eritrocita kao nosača lekova, metoda protočne citometrije je, potvrdivši prisustvo osmotski različitih frakcija eritrocita, ukazala na to da se različite količine inkapsuliranog leka u pojedinačnim, kako svinjskim, tako i goveđim eritrocitima mogu očekivati. PB - Association of Chemical Engineers of Serbia T2 - Hemijska industrija T1 - Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery T1 - Određivanje osmotskih osobina životinjskih eritrocita protočnom citometrijom u cilju njihovog inženjeringa kao nosača lekova EP - 76 IS - 1 SP - 67 VL - 69 DO - 10.2298/HEMIND140124021K ER -
@article{ author = "Kostić, Ivana T. and Ilić, Vesna Lj. and Bukara, Katarina and Mojsilović, Slavko B. and Đurić, Zorka Ž. and Draškovič, Petra and Bugarski, Branko", year = "2015", abstract = "Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bovine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected., Uprkos činjenici da su metode koje se baziraju na osmotskim osobinama ćelija najčešće korišćene metode za inkapsulaciju lekova u humane i životinjske eritrocite, podaci o osmotskim osobinama eritrocita životinjskog porekla su vrlo oskudni. Cilj ovog rada bio je ispitivanje mogućnosti korišćenja metode protočne citometrije za određivanje osmotskih osobina svinjskih i goveđih eritrocita, čime bi se olakšao inženjering pomenutih životinjskih eritrocita za otpuštanje lekova. Metodom protočne citometrije uspešno su dobijene informacije o osmotskoj fragilnosti svinjskih i goveđih eritrocita i načinjeni su početni koraci u proceni oblika velikog broja eritrocita. Iako ova metoda nije uspela da potvrdi bubrenje svinjskih eritrocita, ukazala je na razliku u uzorcima svinjskih eritrocita koji su imali osnovne hematološke parametre izvan i unutar referentnih vrednosti. U cilju primene svinjskih i goveđih eritrocita kao nosača lekova, metoda protočne citometrije je, potvrdivši prisustvo osmotski različitih frakcija eritrocita, ukazala na to da se različite količine inkapsuliranog leka u pojedinačnim, kako svinjskim, tako i goveđim eritrocitima mogu očekivati.", publisher = "Association of Chemical Engineers of Serbia", journal = "Hemijska industrija", title = "Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery, Određivanje osmotskih osobina životinjskih eritrocita protočnom citometrijom u cilju njihovog inženjeringa kao nosača lekova", pages = "76-67", number = "1", volume = "69", doi = "10.2298/HEMIND140124021K" }
Kostić, I. T., Ilić, V. Lj., Bukara, K., Mojsilović, S. B., Đurić, Z. Ž., Draškovič, P.,& Bugarski, B.. (2015). Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery. in Hemijska industrija Association of Chemical Engineers of Serbia., 69(1), 67-76. https://doi.org/10.2298/HEMIND140124021K
Kostić IT, Ilić VL, Bukara K, Mojsilović SB, Đurić ZŽ, Draškovič P, Bugarski B. Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery. in Hemijska industrija. 2015;69(1):67-76. doi:10.2298/HEMIND140124021K .
Kostić, Ivana T., Ilić, Vesna Lj., Bukara, Katarina, Mojsilović, Slavko B., Đurić, Zorka Ž., Draškovič, Petra, Bugarski, Branko, "Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery" in Hemijska industrija, 69, no. 1 (2015):67-76, https://doi.org/10.2298/HEMIND140124021K . .