Nanomaterial with High Antimicrobial Efficacy-Copper/Polyaniline Nanocomposite
Authorized Users Only
2015
Authors
Bogdanović, UnaVodnik, Vesna
Mitrić, Miodrag
Dimitrijević, Suzana
Škapin, Srečo Davor
Zunić, Vojka
Budimir, Milica
Stoiljković, Milovan
Article (Published version)
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Show full item recordAbstract
This study explores different mechanisms of antimicrobial action by designing hybrid nanomaterials that provide a new approach in the fight against resistant microbes. Here, we present a cheap copper-polyaniline (Cu-PANI) nanocomposite material with enhanced antimicrobial properties, prepared by simple in situ polymerization method, when polymer and metal nanoparticles are produced simultaneously. The copper nanoparticles (CuNPs) are uniformly dispersed in the polymer and have a narrow size distribution (dav = 6 nm). We found that CuNPs and PANI act synergistically against three strains, Escherichia coli, Staphylococcus aureus, and Candida albicans, and resulting nanocomposite exhibits higher antimicrobial activity than any component acting alone. Before using the colony counting method to quantify its time and concentration antimicrobial activity, different techniques (UV-visible spectroscopy, transmission electron microscopy, scanning electron microscope, field emission scanning elec...tron microscopy, X-ray diffraction, Fourier transform infrared spectrophotometry, and inductively coupled plasma optical emission spectrometry) were used to identify the optical, structural, and chemical aspects of the formed Cu-PANI nanocomposite. The antimicrobial activity of this nanocomposite shows that the microbial growth has been fully inhibited; moreover, some of the tested microbes were killed. Atomic force microscopy revealed dramatic changes in morphology of tested cells due to disruption of their cell wall integrity after incubation with Cu-PANI nanocomposite.
Keywords:
copper nanoparticles / polyaniline / nanocomposite / antimicrobial activity / AFMSource:
ACS Applied Materials & Interfaces, 2015, 7, 3, 1955-1966Publisher:
- Amer Chemical Soc, Washington
Funding / projects:
- Size-, shape- and structure- dependent properties of nanoparticles and nanocomposites (RS-MESTD-Basic Research (BR or ON)-172056)
- Application of biotechnological methods for sustainable exploitation of by-products of agro-industry (RS-MESTD-Technological Development (TD or TR)-31035)
DOI: 10.1021/am507746m
ISSN: 1944-8244
PubMed: 25552193
WoS: 000348688700070
Scopus: 2-s2.0-84921813337
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Bogdanović, Una AU - Vodnik, Vesna AU - Mitrić, Miodrag AU - Dimitrijević, Suzana AU - Škapin, Srečo Davor AU - Zunić, Vojka AU - Budimir, Milica AU - Stoiljković, Milovan PY - 2015 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3040 AB - This study explores different mechanisms of antimicrobial action by designing hybrid nanomaterials that provide a new approach in the fight against resistant microbes. Here, we present a cheap copper-polyaniline (Cu-PANI) nanocomposite material with enhanced antimicrobial properties, prepared by simple in situ polymerization method, when polymer and metal nanoparticles are produced simultaneously. The copper nanoparticles (CuNPs) are uniformly dispersed in the polymer and have a narrow size distribution (dav = 6 nm). We found that CuNPs and PANI act synergistically against three strains, Escherichia coli, Staphylococcus aureus, and Candida albicans, and resulting nanocomposite exhibits higher antimicrobial activity than any component acting alone. Before using the colony counting method to quantify its time and concentration antimicrobial activity, different techniques (UV-visible spectroscopy, transmission electron microscopy, scanning electron microscope, field emission scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectrophotometry, and inductively coupled plasma optical emission spectrometry) were used to identify the optical, structural, and chemical aspects of the formed Cu-PANI nanocomposite. The antimicrobial activity of this nanocomposite shows that the microbial growth has been fully inhibited; moreover, some of the tested microbes were killed. Atomic force microscopy revealed dramatic changes in morphology of tested cells due to disruption of their cell wall integrity after incubation with Cu-PANI nanocomposite. PB - Amer Chemical Soc, Washington T2 - ACS Applied Materials & Interfaces T1 - Nanomaterial with High Antimicrobial Efficacy-Copper/Polyaniline Nanocomposite EP - 1966 IS - 3 SP - 1955 VL - 7 DO - 10.1021/am507746m ER -
@article{ author = "Bogdanović, Una and Vodnik, Vesna and Mitrić, Miodrag and Dimitrijević, Suzana and Škapin, Srečo Davor and Zunić, Vojka and Budimir, Milica and Stoiljković, Milovan", year = "2015", abstract = "This study explores different mechanisms of antimicrobial action by designing hybrid nanomaterials that provide a new approach in the fight against resistant microbes. Here, we present a cheap copper-polyaniline (Cu-PANI) nanocomposite material with enhanced antimicrobial properties, prepared by simple in situ polymerization method, when polymer and metal nanoparticles are produced simultaneously. The copper nanoparticles (CuNPs) are uniformly dispersed in the polymer and have a narrow size distribution (dav = 6 nm). We found that CuNPs and PANI act synergistically against three strains, Escherichia coli, Staphylococcus aureus, and Candida albicans, and resulting nanocomposite exhibits higher antimicrobial activity than any component acting alone. Before using the colony counting method to quantify its time and concentration antimicrobial activity, different techniques (UV-visible spectroscopy, transmission electron microscopy, scanning electron microscope, field emission scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectrophotometry, and inductively coupled plasma optical emission spectrometry) were used to identify the optical, structural, and chemical aspects of the formed Cu-PANI nanocomposite. The antimicrobial activity of this nanocomposite shows that the microbial growth has been fully inhibited; moreover, some of the tested microbes were killed. Atomic force microscopy revealed dramatic changes in morphology of tested cells due to disruption of their cell wall integrity after incubation with Cu-PANI nanocomposite.", publisher = "Amer Chemical Soc, Washington", journal = "ACS Applied Materials & Interfaces", title = "Nanomaterial with High Antimicrobial Efficacy-Copper/Polyaniline Nanocomposite", pages = "1966-1955", number = "3", volume = "7", doi = "10.1021/am507746m" }
Bogdanović, U., Vodnik, V., Mitrić, M., Dimitrijević, S., Škapin, S. D., Zunić, V., Budimir, M.,& Stoiljković, M.. (2015). Nanomaterial with High Antimicrobial Efficacy-Copper/Polyaniline Nanocomposite. in ACS Applied Materials & Interfaces Amer Chemical Soc, Washington., 7(3), 1955-1966. https://doi.org/10.1021/am507746m
Bogdanović U, Vodnik V, Mitrić M, Dimitrijević S, Škapin SD, Zunić V, Budimir M, Stoiljković M. Nanomaterial with High Antimicrobial Efficacy-Copper/Polyaniline Nanocomposite. in ACS Applied Materials & Interfaces. 2015;7(3):1955-1966. doi:10.1021/am507746m .
Bogdanović, Una, Vodnik, Vesna, Mitrić, Miodrag, Dimitrijević, Suzana, Škapin, Srečo Davor, Zunić, Vojka, Budimir, Milica, Stoiljković, Milovan, "Nanomaterial with High Antimicrobial Efficacy-Copper/Polyaniline Nanocomposite" in ACS Applied Materials & Interfaces, 7, no. 3 (2015):1955-1966, https://doi.org/10.1021/am507746m . .