In vitro conservation of cherry rootstock Gisela 5
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The paper presents results of the application of 'Cold Storage' (CS), a very simple in vitro technique preservation of cultures at +5 degrees C in total darkness. A protocol has been developed for in vitro preservation of cherry rootstock Gisela 5 based on this method. Upon the establishment of aseptic culture, the studied genotype was propagated in vitro on MS medium supplemented with BA at a concentration of 3.37 mg/L. During CS, in vitro shoots were maintained at +5 degrees C in cold chamber for 3, 6 and 9 months in total darkness. Seven days after their respective period of time, the shoots were examined for viability for further propagation, together with their multiplication index and length of axial and lateral shoots. Three months after CS, shoots showed high shoot viability (55%), which however declined considerably after 6 and 9 months (15% and 0%, resp.). After 9 months of preservation under cold conditions, shoots showed severe signs of necrosis (55%). The transfer of cultu...res from the cold chamber to standard growth conditions led to prompt development and greening of leaves which regained morphology and capacity for multiplication and rooting.
Ključne reči:
In vitro conservation / Cherry / Rootstock / Cold storage / Propagation / Germplasm preservationIzvor:
Indian Journal of Traditional Knowledge, 2015, 14, 2, 191-197Izdavač:
- Natl Inst Science Communication-Niscair, New Delhi
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Institucija/grupa
Inovacioni centarTY - JOUR AU - Ružić, Đurđina AU - Vujović, Tatjana AU - Cerović, Radosav PY - 2015 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3136 AB - The paper presents results of the application of 'Cold Storage' (CS), a very simple in vitro technique preservation of cultures at +5 degrees C in total darkness. A protocol has been developed for in vitro preservation of cherry rootstock Gisela 5 based on this method. Upon the establishment of aseptic culture, the studied genotype was propagated in vitro on MS medium supplemented with BA at a concentration of 3.37 mg/L. During CS, in vitro shoots were maintained at +5 degrees C in cold chamber for 3, 6 and 9 months in total darkness. Seven days after their respective period of time, the shoots were examined for viability for further propagation, together with their multiplication index and length of axial and lateral shoots. Three months after CS, shoots showed high shoot viability (55%), which however declined considerably after 6 and 9 months (15% and 0%, resp.). After 9 months of preservation under cold conditions, shoots showed severe signs of necrosis (55%). The transfer of cultures from the cold chamber to standard growth conditions led to prompt development and greening of leaves which regained morphology and capacity for multiplication and rooting. PB - Natl Inst Science Communication-Niscair, New Delhi T2 - Indian Journal of Traditional Knowledge T1 - In vitro conservation of cherry rootstock Gisela 5 EP - 197 IS - 2 SP - 191 VL - 14 UR - https://hdl.handle.net/21.15107/rcub_technorep_3136 ER -
@article{ author = "Ružić, Đurđina and Vujović, Tatjana and Cerović, Radosav", year = "2015", abstract = "The paper presents results of the application of 'Cold Storage' (CS), a very simple in vitro technique preservation of cultures at +5 degrees C in total darkness. A protocol has been developed for in vitro preservation of cherry rootstock Gisela 5 based on this method. Upon the establishment of aseptic culture, the studied genotype was propagated in vitro on MS medium supplemented with BA at a concentration of 3.37 mg/L. During CS, in vitro shoots were maintained at +5 degrees C in cold chamber for 3, 6 and 9 months in total darkness. Seven days after their respective period of time, the shoots were examined for viability for further propagation, together with their multiplication index and length of axial and lateral shoots. Three months after CS, shoots showed high shoot viability (55%), which however declined considerably after 6 and 9 months (15% and 0%, resp.). After 9 months of preservation under cold conditions, shoots showed severe signs of necrosis (55%). The transfer of cultures from the cold chamber to standard growth conditions led to prompt development and greening of leaves which regained morphology and capacity for multiplication and rooting.", publisher = "Natl Inst Science Communication-Niscair, New Delhi", journal = "Indian Journal of Traditional Knowledge", title = "In vitro conservation of cherry rootstock Gisela 5", pages = "197-191", number = "2", volume = "14", url = "https://hdl.handle.net/21.15107/rcub_technorep_3136" }
Ružić, Đ., Vujović, T.,& Cerović, R.. (2015). In vitro conservation of cherry rootstock Gisela 5. in Indian Journal of Traditional Knowledge Natl Inst Science Communication-Niscair, New Delhi., 14(2), 191-197. https://hdl.handle.net/21.15107/rcub_technorep_3136
Ružić Đ, Vujović T, Cerović R. In vitro conservation of cherry rootstock Gisela 5. in Indian Journal of Traditional Knowledge. 2015;14(2):191-197. https://hdl.handle.net/21.15107/rcub_technorep_3136 .
Ružić, Đurđina, Vujović, Tatjana, Cerović, Radosav, "In vitro conservation of cherry rootstock Gisela 5" in Indian Journal of Traditional Knowledge, 14, no. 2 (2015):191-197, https://hdl.handle.net/21.15107/rcub_technorep_3136 .