Lignocellulosic waste material as substrate for Avicelase production by a new strain of Paenibacillus chitinolyticus CKS1
Само за регистроване кориснике
2015
Аутори
Mihajlovski, KatarinaCarević, Milica
Dević, Marija Lj.
Šiler-Marinković, Slavica
Rajilić-Stojanović, Mirjana
Dimitrijević-Branković, Suzana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A novel strain of Paenibacillus chitinolyticus CKS1 was isolated from forest soil and identified as a potent cellulase producer. The strain was able to grow on various commercial substrates including microcrystalline cellulose (Avicel), carboxymethylcellulose (CMC), and cellobiose but also on lignocellulosic waste material such as medicinal herbs waste and sawdust. On all these substrates the strain produced cellulase composed of two subunits (similar to 70 and similar to 45 kDa) that was active on CMC, Avicel and filter paper. The maximal Avicelase activity (1.94 U/ml) was reached in a medium that contained 0.1% (w/v) of medicinal herbs waste, 3 g l(-1) of yeast extract and 5.0 g l(-1) of casein hydrolysate in 0.1 M phosphate buffer pH 7, after 48 h of incubation at 30 degrees C. The Avicelase performed optimally at 80 degrees C and at pH 4.8. Addition of K+ increased the Avicelase activity almost three fold and the enzyme retained 48.39% of the initial activity after 60 min. The prod...uct of Avicel and CMC hydrolysis was glucose with traces of other soluble sugars, indicating that the crude cellulase produced on waste material using the novel P. chitinolyticus strain CKS 1 could be used in eco-friendly processes of cellulose bioconversion, such as enzymatic saccharification of lignocellulosic materials in processes performed under acidophilic conditions and high temperatures.
Кључне речи:
Cellulase / Paenibacillus chitinolyticus CKS1 / Avicelase / Medicinal herbs wasteИзвор:
International Biodeterioration & Biodegradation, 2015, 104, 426-434Издавач:
- Elsevier Sci Ltd, Oxford
Финансирање / пројекти:
- Примена биотехнолошких метода у одрживом искоришћењу нус-производа агроиндустрије (RS-MESTD-Technological Development (TD or TR)-31035)
DOI: 10.1016/j.ibiod.2015.07.012
ISSN: 0964-8305
WoS: 000362308400057
Scopus: 2-s2.0-84938695125
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Mihajlovski, Katarina AU - Carević, Milica AU - Dević, Marija Lj. AU - Šiler-Marinković, Slavica AU - Rajilić-Stojanović, Mirjana AU - Dimitrijević-Branković, Suzana PY - 2015 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3137 AB - A novel strain of Paenibacillus chitinolyticus CKS1 was isolated from forest soil and identified as a potent cellulase producer. The strain was able to grow on various commercial substrates including microcrystalline cellulose (Avicel), carboxymethylcellulose (CMC), and cellobiose but also on lignocellulosic waste material such as medicinal herbs waste and sawdust. On all these substrates the strain produced cellulase composed of two subunits (similar to 70 and similar to 45 kDa) that was active on CMC, Avicel and filter paper. The maximal Avicelase activity (1.94 U/ml) was reached in a medium that contained 0.1% (w/v) of medicinal herbs waste, 3 g l(-1) of yeast extract and 5.0 g l(-1) of casein hydrolysate in 0.1 M phosphate buffer pH 7, after 48 h of incubation at 30 degrees C. The Avicelase performed optimally at 80 degrees C and at pH 4.8. Addition of K+ increased the Avicelase activity almost three fold and the enzyme retained 48.39% of the initial activity after 60 min. The product of Avicel and CMC hydrolysis was glucose with traces of other soluble sugars, indicating that the crude cellulase produced on waste material using the novel P. chitinolyticus strain CKS 1 could be used in eco-friendly processes of cellulose bioconversion, such as enzymatic saccharification of lignocellulosic materials in processes performed under acidophilic conditions and high temperatures. PB - Elsevier Sci Ltd, Oxford T2 - International Biodeterioration & Biodegradation T1 - Lignocellulosic waste material as substrate for Avicelase production by a new strain of Paenibacillus chitinolyticus CKS1 EP - 434 SP - 426 VL - 104 DO - 10.1016/j.ibiod.2015.07.012 ER -
@article{ author = "Mihajlovski, Katarina and Carević, Milica and Dević, Marija Lj. and Šiler-Marinković, Slavica and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana", year = "2015", abstract = "A novel strain of Paenibacillus chitinolyticus CKS1 was isolated from forest soil and identified as a potent cellulase producer. The strain was able to grow on various commercial substrates including microcrystalline cellulose (Avicel), carboxymethylcellulose (CMC), and cellobiose but also on lignocellulosic waste material such as medicinal herbs waste and sawdust. On all these substrates the strain produced cellulase composed of two subunits (similar to 70 and similar to 45 kDa) that was active on CMC, Avicel and filter paper. The maximal Avicelase activity (1.94 U/ml) was reached in a medium that contained 0.1% (w/v) of medicinal herbs waste, 3 g l(-1) of yeast extract and 5.0 g l(-1) of casein hydrolysate in 0.1 M phosphate buffer pH 7, after 48 h of incubation at 30 degrees C. The Avicelase performed optimally at 80 degrees C and at pH 4.8. Addition of K+ increased the Avicelase activity almost three fold and the enzyme retained 48.39% of the initial activity after 60 min. The product of Avicel and CMC hydrolysis was glucose with traces of other soluble sugars, indicating that the crude cellulase produced on waste material using the novel P. chitinolyticus strain CKS 1 could be used in eco-friendly processes of cellulose bioconversion, such as enzymatic saccharification of lignocellulosic materials in processes performed under acidophilic conditions and high temperatures.", publisher = "Elsevier Sci Ltd, Oxford", journal = "International Biodeterioration & Biodegradation", title = "Lignocellulosic waste material as substrate for Avicelase production by a new strain of Paenibacillus chitinolyticus CKS1", pages = "434-426", volume = "104", doi = "10.1016/j.ibiod.2015.07.012" }
Mihajlovski, K., Carević, M., Dević, M. Lj., Šiler-Marinković, S., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2015). Lignocellulosic waste material as substrate for Avicelase production by a new strain of Paenibacillus chitinolyticus CKS1. in International Biodeterioration & Biodegradation Elsevier Sci Ltd, Oxford., 104, 426-434. https://doi.org/10.1016/j.ibiod.2015.07.012
Mihajlovski K, Carević M, Dević ML, Šiler-Marinković S, Rajilić-Stojanović M, Dimitrijević-Branković S. Lignocellulosic waste material as substrate for Avicelase production by a new strain of Paenibacillus chitinolyticus CKS1. in International Biodeterioration & Biodegradation. 2015;104:426-434. doi:10.1016/j.ibiod.2015.07.012 .
Mihajlovski, Katarina, Carević, Milica, Dević, Marija Lj., Šiler-Marinković, Slavica, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Lignocellulosic waste material as substrate for Avicelase production by a new strain of Paenibacillus chitinolyticus CKS1" in International Biodeterioration & Biodegradation, 104 (2015):426-434, https://doi.org/10.1016/j.ibiod.2015.07.012 . .