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In vitro cell response to Co-containing 1393 bioactive glass

Authorized Users Only
2015
Authors
Hoppe, Alexander
Brandl, Andreas
Bleiziffer, Oliver
Arkudas, Andreas
Horch, Raymund E.
Jokić, Bojan
Janaćković, Đorđe
Boccaccini, Aldo R.
Article (Published version)
Metadata
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Abstract
Cobalt ions are known to stimulate angiogenesis via inducing hypoxic conditions and hence are interesting agents to be used in conjunction with bioactive glasses (BGs) in bone tissue engineering approaches. In this work we investigated in vitro cell biocompatibility of Co releasing 1393 BG composition (in wt.%: 53SiO(2), 6Na(2)O, 12K(2)O, 5MgO, 20CaO, and 4P(2)O(5)) derived scaffolds with osteoblast-like cells (MG-63) and human dermal microvascular endothelial cells (hDMECs). Cell viability, cell number and cell morphology of osteoblast-like cells in contact with particulate glass and 3D scaffolds were assessed showing good biocompatibility of 1393 reference material and with 1 wt.% CoO addition whereby 5 wt.% of CoO in the glass showed cytotoxicity. Furthermore for 1393 with 1 wt.% of CoO increased mitochondrial activity was measured. Similar observations were made with hDMECs: while 1393 and 1393 with 1 wt.% CoO were biocompatible and the endothelial phenotype was retained, 5 wt.% Co...O containing BG showed cytotoxic effects after 1 week of cell culture. In conclusion, 1 wt.% Co containing BG was biocompatible with osteoblast like cells and endothelial cells and showed slightly stimulating effects on osteoblast-like cells whereas the addition of 5 wt.% CoO seems to exceed the vital therapeutic ranges of Co ions being released in physiological fluids.

Keywords:
Cobalt / Bioactive glass / Angiogenesis / Endothelial cells
Source:
Materials Science & Engineering C-Materials for Biological Applications, 2015, 57, 157-163
Publisher:
  • Elsevier Science Bv, Amsterdam
Funding / projects:
  • Emerging Fields Initiative (EFI) of the University of Erlangen-Nurnberg [Tech_06 TOPbiomat]

DOI: 10.1016/j.msec.2015.07.014

ISSN: 0928-4931

PubMed: 26354250

WoS: 000361779400018

Scopus: 2-s2.0-84938389346
[ Google Scholar ]
27
24
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3142
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
Institution/Community
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Hoppe, Alexander
AU  - Brandl, Andreas
AU  - Bleiziffer, Oliver
AU  - Arkudas, Andreas
AU  - Horch, Raymund E.
AU  - Jokić, Bojan
AU  - Janaćković, Đorđe
AU  - Boccaccini, Aldo R.
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3142
AB  - Cobalt ions are known to stimulate angiogenesis via inducing hypoxic conditions and hence are interesting agents to be used in conjunction with bioactive glasses (BGs) in bone tissue engineering approaches. In this work we investigated in vitro cell biocompatibility of Co releasing 1393 BG composition (in wt.%: 53SiO(2), 6Na(2)O, 12K(2)O, 5MgO, 20CaO, and 4P(2)O(5)) derived scaffolds with osteoblast-like cells (MG-63) and human dermal microvascular endothelial cells (hDMECs). Cell viability, cell number and cell morphology of osteoblast-like cells in contact with particulate glass and 3D scaffolds were assessed showing good biocompatibility of 1393 reference material and with 1 wt.% CoO addition whereby 5 wt.% of CoO in the glass showed cytotoxicity. Furthermore for 1393 with 1 wt.% of CoO increased mitochondrial activity was measured. Similar observations were made with hDMECs: while 1393 and 1393 with 1 wt.% CoO were biocompatible and the endothelial phenotype was retained, 5 wt.% CoO containing BG showed cytotoxic effects after 1 week of cell culture. In conclusion, 1 wt.% Co containing BG was biocompatible with osteoblast like cells and endothelial cells and showed slightly stimulating effects on osteoblast-like cells whereas the addition of 5 wt.% CoO seems to exceed the vital therapeutic ranges of Co ions being released in physiological fluids.
PB  - Elsevier Science Bv, Amsterdam
T2  - Materials Science & Engineering C-Materials for Biological Applications
T1  - In vitro cell response to Co-containing 1393 bioactive glass
EP  - 163
SP  - 157
VL  - 57
DO  - 10.1016/j.msec.2015.07.014
UR  - conv_4796
ER  - 
@article{
author = "Hoppe, Alexander and Brandl, Andreas and Bleiziffer, Oliver and Arkudas, Andreas and Horch, Raymund E. and Jokić, Bojan and Janaćković, Đorđe and Boccaccini, Aldo R.",
year = "2015",
abstract = "Cobalt ions are known to stimulate angiogenesis via inducing hypoxic conditions and hence are interesting agents to be used in conjunction with bioactive glasses (BGs) in bone tissue engineering approaches. In this work we investigated in vitro cell biocompatibility of Co releasing 1393 BG composition (in wt.%: 53SiO(2), 6Na(2)O, 12K(2)O, 5MgO, 20CaO, and 4P(2)O(5)) derived scaffolds with osteoblast-like cells (MG-63) and human dermal microvascular endothelial cells (hDMECs). Cell viability, cell number and cell morphology of osteoblast-like cells in contact with particulate glass and 3D scaffolds were assessed showing good biocompatibility of 1393 reference material and with 1 wt.% CoO addition whereby 5 wt.% of CoO in the glass showed cytotoxicity. Furthermore for 1393 with 1 wt.% of CoO increased mitochondrial activity was measured. Similar observations were made with hDMECs: while 1393 and 1393 with 1 wt.% CoO were biocompatible and the endothelial phenotype was retained, 5 wt.% CoO containing BG showed cytotoxic effects after 1 week of cell culture. In conclusion, 1 wt.% Co containing BG was biocompatible with osteoblast like cells and endothelial cells and showed slightly stimulating effects on osteoblast-like cells whereas the addition of 5 wt.% CoO seems to exceed the vital therapeutic ranges of Co ions being released in physiological fluids.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Materials Science & Engineering C-Materials for Biological Applications",
title = "In vitro cell response to Co-containing 1393 bioactive glass",
pages = "163-157",
volume = "57",
doi = "10.1016/j.msec.2015.07.014",
url = "conv_4796"
}
Hoppe, A., Brandl, A., Bleiziffer, O., Arkudas, A., Horch, R. E., Jokić, B., Janaćković, Đ.,& Boccaccini, A. R.. (2015). In vitro cell response to Co-containing 1393 bioactive glass. in Materials Science & Engineering C-Materials for Biological Applications
Elsevier Science Bv, Amsterdam., 57, 157-163.
https://doi.org/10.1016/j.msec.2015.07.014
conv_4796
Hoppe A, Brandl A, Bleiziffer O, Arkudas A, Horch RE, Jokić B, Janaćković Đ, Boccaccini AR. In vitro cell response to Co-containing 1393 bioactive glass. in Materials Science & Engineering C-Materials for Biological Applications. 2015;57:157-163.
doi:10.1016/j.msec.2015.07.014
conv_4796 .
Hoppe, Alexander, Brandl, Andreas, Bleiziffer, Oliver, Arkudas, Andreas, Horch, Raymund E., Jokić, Bojan, Janaćković, Đorđe, Boccaccini, Aldo R., "In vitro cell response to Co-containing 1393 bioactive glass" in Materials Science & Engineering C-Materials for Biological Applications, 57 (2015):157-163,
https://doi.org/10.1016/j.msec.2015.07.014 .,
conv_4796 .

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