Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.
TY - JOUR
AU - Jugović, Branimir
AU - Grgur, Branimir
AU - Antov, Mirjana
AU - Knežević-Jugović, Zorica
AU - Stevanović, Jasmina
AU - Gvozdenović, Milica
PY - 2016
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3184
AB - Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.
PB - Electrochemical Science Group, Beograd
T2 - International Journal of Electrochemical Science
T1 - Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose
EP - 1161
IS - 2
SP - 1152
VL - 11
ER -
@article{
author = "Jugović, Branimir and Grgur, Branimir and Antov, Mirjana and Knežević-Jugović, Zorica and Stevanović, Jasmina and Gvozdenović, Milica",
year = "2016",
url = "http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3184",
abstract = "Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.",
publisher = "Electrochemical Science Group, Beograd",
journal = "International Journal of Electrochemical Science",
title = "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose",
pages = "1161-1152",
number = "2",
volume = "11"
}
Jugović, B., Grgur, B., Antov, M., Knežević-Jugović, Z., Stevanović, J.,& Gvozdenović, M. (2016). Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose.
International Journal of Electrochemical Science
Electrochemical Science Group, Beograd., 11(2), 1152-1161.
Jugović B, Grgur B, Antov M, Knežević-Jugović Z, Stevanović J, Gvozdenović M. Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. International Journal of Electrochemical Science. 2016;11(2):1152-1161
Jugović Branimir, Grgur Branimir, Antov Mirjana, Knežević-Jugović Zorica, Stevanović Jasmina, Gvozdenović Milica, "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose" International Journal of Electrochemical Science, 11, no. 2 (2016):1152-1161
