Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose
Само за регистроване кориснике
2016
Аутори
Jugović, BranimirGrgur, Branimir
Antov, Mirjana
Knežević-Jugović, Zorica
Stevanović, Jasmina
Gvozdenović, Milica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.
Кључне речи:
conducting polymers / electrochemistry / glucose oxidase / material testing / sensorsИзвор:
International Journal of Electrochemical Science, 2016, 11, 2, 1152-1161Издавач:
- Electrochemical Science Group, Beograd
Финансирање / пројекти:
- Електрохемијска синтеза и карактеризација наноструктурираних функционалних материјала за примену у новим технологијама (RS-MESTD-Basic Research (BR or ON)-172046)
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Jugović, Branimir AU - Grgur, Branimir AU - Antov, Mirjana AU - Knežević-Jugović, Zorica AU - Stevanović, Jasmina AU - Gvozdenović, Milica PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3184 AB - Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix. PB - Electrochemical Science Group, Beograd T2 - International Journal of Electrochemical Science T1 - Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose EP - 1161 IS - 2 SP - 1152 VL - 11 UR - https://hdl.handle.net/21.15107/rcub_technorep_3184 ER -
@article{ author = "Jugović, Branimir and Grgur, Branimir and Antov, Mirjana and Knežević-Jugović, Zorica and Stevanović, Jasmina and Gvozdenović, Milica", year = "2016", abstract = "Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.", publisher = "Electrochemical Science Group, Beograd", journal = "International Journal of Electrochemical Science", title = "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose", pages = "1161-1152", number = "2", volume = "11", url = "https://hdl.handle.net/21.15107/rcub_technorep_3184" }
Jugović, B., Grgur, B., Antov, M., Knežević-Jugović, Z., Stevanović, J.,& Gvozdenović, M.. (2016). Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. in International Journal of Electrochemical Science Electrochemical Science Group, Beograd., 11(2), 1152-1161. https://hdl.handle.net/21.15107/rcub_technorep_3184
Jugović B, Grgur B, Antov M, Knežević-Jugović Z, Stevanović J, Gvozdenović M. Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. in International Journal of Electrochemical Science. 2016;11(2):1152-1161. https://hdl.handle.net/21.15107/rcub_technorep_3184 .
Jugović, Branimir, Grgur, Branimir, Antov, Mirjana, Knežević-Jugović, Zorica, Stevanović, Jasmina, Gvozdenović, Milica, "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose" in International Journal of Electrochemical Science, 11, no. 2 (2016):1152-1161, https://hdl.handle.net/21.15107/rcub_technorep_3184 .