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Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose

Samo za registrovane korisnike
2016
Autori
Jugović, Branimir
Grgur, Branimir
Antov, Mirjana
Knežević-Jugović, Zorica
Stevanović, Jasmina
Gvozdenović, Milica
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentu
Apstrakt
Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.
Ključne reči:
conducting polymers / electrochemistry / glucose oxidase / material testing / sensors
Izvor:
International Journal of Electrochemical Science, 2016, 11, 2, 1152-1161
Izdavač:
  • Electrochemical Science Group, Beograd
Finansiranje / projekti:
  • Elektrohemijska sinteza i karakterizacija nanostrukturiranih funkcionalnih materijala za primenu u novim tehnologijama (RS-172046)

ISSN: 1452-3981

PubMed:

WoS: 000371091400024

Scopus: 2-s2.0-84958225235
[ Google Scholar ]
18
18
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3184
Kolekcije
  • Radovi istraživača / Researchers’ publications (TMF)
Institucija/grupa
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Jugović, Branimir
AU  - Grgur, Branimir
AU  - Antov, Mirjana
AU  - Knežević-Jugović, Zorica
AU  - Stevanović, Jasmina
AU  - Gvozdenović, Milica
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3184
AB  - Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.
PB  - Electrochemical Science Group, Beograd
T2  - International Journal of Electrochemical Science
T1  - Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose
EP  - 1161
IS  - 2
SP  - 1152
VL  - 11
UR  - conv_6496
ER  - 
@article{
author = "Jugović, Branimir and Grgur, Branimir and Antov, Mirjana and Knežević-Jugović, Zorica and Stevanović, Jasmina and Gvozdenović, Milica",
year = "2016",
abstract = "Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.",
publisher = "Electrochemical Science Group, Beograd",
journal = "International Journal of Electrochemical Science",
title = "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose",
pages = "1161-1152",
number = "2",
volume = "11",
url = "conv_6496"
}
Jugović, B., Grgur, B., Antov, M., Knežević-Jugović, Z., Stevanović, J.,& Gvozdenović, M.. (2016). Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. in International Journal of Electrochemical Science
Electrochemical Science Group, Beograd., 11(2), 1152-1161.
conv_6496
Jugović B, Grgur B, Antov M, Knežević-Jugović Z, Stevanović J, Gvozdenović M. Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. in International Journal of Electrochemical Science. 2016;11(2):1152-1161.
conv_6496 .
Jugović, Branimir, Grgur, Branimir, Antov, Mirjana, Knežević-Jugović, Zorica, Stevanović, Jasmina, Gvozdenović, Milica, "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose" in International Journal of Electrochemical Science, 11, no. 2 (2016):1152-1161,
conv_6496 .

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