Immobilization of horseradish peroxidase onto kaolin by glutaraldehyde method and its application in decolorization of anthraquinone dye
2016
Аутори
Šekuljica, NatašaPrlainović, Nevena
Jovanović, Jelena
Stefanović, Andrea
Grbavčić, Sanja
Mijin, Dušan
Knežević-Jugović, Zorica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The problem of environmental pollution becomes more worrisome day by day, primarily due to the large amounts of wastewater contaminated with various harmful organic compounds, discharged untreated or partially clean into the environment. Feasibility of use of horseradish peroxidase (Amoracia rusticana) in the synthetic dyes decolorization was approved by many researchers. Among a number of supports used for the immobilization, it was found that natural clay, kaolin, has excellent features which are a precondition for obtaining biocatalysts with the excellent performances. For this reason, a horseradish peroxidase was immobilized onto kaolin using glutaraldehyde as a cross-linking agent. Obtained biocatalyst was applied in the decolorization of anthraquinone dye C.I. acid violet 109. Under determined optimal conditions (pH 4.0, hydrogen peroxide concentration 0.6 mM, dye concentration 30 mg L-1, temperature 24 degrees C) around 76% of dye decolorization was achieved. Reusability study s...howed that resulting biocatalyst was possible to apply four times in the desired reaction with relatively high decolorization percentage.
Кључне речи:
horseradish peroxidase / immobilization / wastewater / dyesИзвор:
Hemijska industrija, 2016, 70, 2, 217-224Издавач:
- Savez hemijskih inženjera, Beograd
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Проучавање синтезе, структуре и активности органских једињења природног и синтетског порекла (RS-MESTD-Basic Research (BR or ON)-172013)
DOI: 10.2298/HEMIND150220028S
ISSN: 0367-598X
WoS: 000376274600012
Scopus: 2-s2.0-84966658477
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Šekuljica, Nataša AU - Prlainović, Nevena AU - Jovanović, Jelena AU - Stefanović, Andrea AU - Grbavčić, Sanja AU - Mijin, Dušan AU - Knežević-Jugović, Zorica PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3251 AB - The problem of environmental pollution becomes more worrisome day by day, primarily due to the large amounts of wastewater contaminated with various harmful organic compounds, discharged untreated or partially clean into the environment. Feasibility of use of horseradish peroxidase (Amoracia rusticana) in the synthetic dyes decolorization was approved by many researchers. Among a number of supports used for the immobilization, it was found that natural clay, kaolin, has excellent features which are a precondition for obtaining biocatalysts with the excellent performances. For this reason, a horseradish peroxidase was immobilized onto kaolin using glutaraldehyde as a cross-linking agent. Obtained biocatalyst was applied in the decolorization of anthraquinone dye C.I. acid violet 109. Under determined optimal conditions (pH 4.0, hydrogen peroxide concentration 0.6 mM, dye concentration 30 mg L-1, temperature 24 degrees C) around 76% of dye decolorization was achieved. Reusability study showed that resulting biocatalyst was possible to apply four times in the desired reaction with relatively high decolorization percentage. PB - Savez hemijskih inženjera, Beograd T2 - Hemijska industrija T1 - Immobilization of horseradish peroxidase onto kaolin by glutaraldehyde method and its application in decolorization of anthraquinone dye EP - 224 IS - 2 SP - 217 VL - 70 DO - 10.2298/HEMIND150220028S ER -
@article{ author = "Šekuljica, Nataša and Prlainović, Nevena and Jovanović, Jelena and Stefanović, Andrea and Grbavčić, Sanja and Mijin, Dušan and Knežević-Jugović, Zorica", year = "2016", abstract = "The problem of environmental pollution becomes more worrisome day by day, primarily due to the large amounts of wastewater contaminated with various harmful organic compounds, discharged untreated or partially clean into the environment. Feasibility of use of horseradish peroxidase (Amoracia rusticana) in the synthetic dyes decolorization was approved by many researchers. Among a number of supports used for the immobilization, it was found that natural clay, kaolin, has excellent features which are a precondition for obtaining biocatalysts with the excellent performances. For this reason, a horseradish peroxidase was immobilized onto kaolin using glutaraldehyde as a cross-linking agent. Obtained biocatalyst was applied in the decolorization of anthraquinone dye C.I. acid violet 109. Under determined optimal conditions (pH 4.0, hydrogen peroxide concentration 0.6 mM, dye concentration 30 mg L-1, temperature 24 degrees C) around 76% of dye decolorization was achieved. Reusability study showed that resulting biocatalyst was possible to apply four times in the desired reaction with relatively high decolorization percentage.", publisher = "Savez hemijskih inženjera, Beograd", journal = "Hemijska industrija", title = "Immobilization of horseradish peroxidase onto kaolin by glutaraldehyde method and its application in decolorization of anthraquinone dye", pages = "224-217", number = "2", volume = "70", doi = "10.2298/HEMIND150220028S" }
Šekuljica, N., Prlainović, N., Jovanović, J., Stefanović, A., Grbavčić, S., Mijin, D.,& Knežević-Jugović, Z.. (2016). Immobilization of horseradish peroxidase onto kaolin by glutaraldehyde method and its application in decolorization of anthraquinone dye. in Hemijska industrija Savez hemijskih inženjera, Beograd., 70(2), 217-224. https://doi.org/10.2298/HEMIND150220028S
Šekuljica N, Prlainović N, Jovanović J, Stefanović A, Grbavčić S, Mijin D, Knežević-Jugović Z. Immobilization of horseradish peroxidase onto kaolin by glutaraldehyde method and its application in decolorization of anthraquinone dye. in Hemijska industrija. 2016;70(2):217-224. doi:10.2298/HEMIND150220028S .
Šekuljica, Nataša, Prlainović, Nevena, Jovanović, Jelena, Stefanović, Andrea, Grbavčić, Sanja, Mijin, Dušan, Knežević-Jugović, Zorica, "Immobilization of horseradish peroxidase onto kaolin by glutaraldehyde method and its application in decolorization of anthraquinone dye" in Hemijska industrija, 70, no. 2 (2016):217-224, https://doi.org/10.2298/HEMIND150220028S . .