Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids
Samo za registrovane korisnike
2016
Autori
Pajović, Jelena D.Dojčilović, Radovan
Božanić, Dušan K.
Vodnik, Vesna
Dimitrijević-Branković, Suzana
Kascakova, Slavka
Refregiers, Matthieu
Markelić, Milica
Đoković, Vladimir
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Fluorescent nanostructures were prepared by functionalization of gold nanoparticles with riboflavin molecules and used as probes for synchrotron radiation deep ultraviolet (SR-DUV) fluorescence imaging of gluteraldehyde-fixed Candida albicans cells. The nanoparticles were characterized by transmission electron microscopy (TEM) and optical spectroscopy techniques. The TEM analysis showed that the nanostructures were 6 nm in diameter, while the results of the fluorescence spectroscopies confirmed the photoluminescence of the nanoparticles. The SR-DUV imaging showed that it was possible to distinguish the fluorescence of the nanoparticles from the autofluorescence of the cells, as well as that the local maxima of the signal pertaining to the fluorescence of goldriboflavin nanostructures were mostly positioned on the surfaces of the C. albicans cells of spherical morphology.
Ključne reči:
Gold nanoparticles / Riboflavin / Functionalization / Candida albicans / Fluorescence imagingIzvor:
Optical and Quantum Electronics, 2016, 48, 6Izdavač:
- Springer, Dordrecht
Finansiranje / projekti:
- [20120810]
- [20131218]
- Uticaj veličine, oblika i strukture nanočestica na njihova svojstva i svojstva nanokompozita (RS-MESTD-Basic Research (BR or ON)-172056)
- Materijali redukovane dimenzionalnosti za efikasnu apsorpciju svetlosti i konverziju energije (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45020)
- Proučavanje uticaja tretiranja na dielektrične, optičke, magnetne i osobine površina kristalnih i polimernih sistema (RS-MESTD-Basic Research (BR or ON)-171029)
DOI: 10.1007/s11082-016-0578-y
ISSN: 0306-8919
WoS: 000377387300004
Scopus: 2-s2.0-84966440446
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Pajović, Jelena D. AU - Dojčilović, Radovan AU - Božanić, Dušan K. AU - Vodnik, Vesna AU - Dimitrijević-Branković, Suzana AU - Kascakova, Slavka AU - Refregiers, Matthieu AU - Markelić, Milica AU - Đoković, Vladimir PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3256 AB - Fluorescent nanostructures were prepared by functionalization of gold nanoparticles with riboflavin molecules and used as probes for synchrotron radiation deep ultraviolet (SR-DUV) fluorescence imaging of gluteraldehyde-fixed Candida albicans cells. The nanoparticles were characterized by transmission electron microscopy (TEM) and optical spectroscopy techniques. The TEM analysis showed that the nanostructures were 6 nm in diameter, while the results of the fluorescence spectroscopies confirmed the photoluminescence of the nanoparticles. The SR-DUV imaging showed that it was possible to distinguish the fluorescence of the nanoparticles from the autofluorescence of the cells, as well as that the local maxima of the signal pertaining to the fluorescence of goldriboflavin nanostructures were mostly positioned on the surfaces of the C. albicans cells of spherical morphology. PB - Springer, Dordrecht T2 - Optical and Quantum Electronics T1 - Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids IS - 6 VL - 48 DO - 10.1007/s11082-016-0578-y ER -
@article{ author = "Pajović, Jelena D. and Dojčilović, Radovan and Božanić, Dušan K. and Vodnik, Vesna and Dimitrijević-Branković, Suzana and Kascakova, Slavka and Refregiers, Matthieu and Markelić, Milica and Đoković, Vladimir", year = "2016", abstract = "Fluorescent nanostructures were prepared by functionalization of gold nanoparticles with riboflavin molecules and used as probes for synchrotron radiation deep ultraviolet (SR-DUV) fluorescence imaging of gluteraldehyde-fixed Candida albicans cells. The nanoparticles were characterized by transmission electron microscopy (TEM) and optical spectroscopy techniques. The TEM analysis showed that the nanostructures were 6 nm in diameter, while the results of the fluorescence spectroscopies confirmed the photoluminescence of the nanoparticles. The SR-DUV imaging showed that it was possible to distinguish the fluorescence of the nanoparticles from the autofluorescence of the cells, as well as that the local maxima of the signal pertaining to the fluorescence of goldriboflavin nanostructures were mostly positioned on the surfaces of the C. albicans cells of spherical morphology.", publisher = "Springer, Dordrecht", journal = "Optical and Quantum Electronics", title = "Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids", number = "6", volume = "48", doi = "10.1007/s11082-016-0578-y" }
Pajović, J. D., Dojčilović, R., Božanić, D. K., Vodnik, V., Dimitrijević-Branković, S., Kascakova, S., Refregiers, M., Markelić, M.,& Đoković, V.. (2016). Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids. in Optical and Quantum Electronics Springer, Dordrecht., 48(6). https://doi.org/10.1007/s11082-016-0578-y
Pajović JD, Dojčilović R, Božanić DK, Vodnik V, Dimitrijević-Branković S, Kascakova S, Refregiers M, Markelić M, Đoković V. Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids. in Optical and Quantum Electronics. 2016;48(6). doi:10.1007/s11082-016-0578-y .
Pajović, Jelena D., Dojčilović, Radovan, Božanić, Dušan K., Vodnik, Vesna, Dimitrijević-Branković, Suzana, Kascakova, Slavka, Refregiers, Matthieu, Markelić, Milica, Đoković, Vladimir, "Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids" in Optical and Quantum Electronics, 48, no. 6 (2016), https://doi.org/10.1007/s11082-016-0578-y . .