Removal of Anthraquinone Dye by Cross-Linked Enzyme Aggregates From Fresh Horseradish Extract
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2016
Authors
Šekuljica, NatašaPrlainović, Nevena
Jakovetić, Sonja
Grbavčić, Sanja
Ognjanović, Nevena
Knežević-Jugović, Zorica
Mijin, Dušan
Article (Published version)
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Horseradish roots (Armoracia rusticana) fresh extract was the source of unpurified horseradish peroxidase (HRP) which was used for the immobilization in a form of cross-linked enzyme aggregates (CLEAs). Among three precipitants used, acetone appeared to be the best choice for the precipitation. Acetone with 2% of glutaraldehyde gave the recovery of 30.3% of the enzyme initial activity. Horseradish peroxidase immobilized in a form of cross-linked enzyme aggregates (HRP-CLEAs) showed remarkable efficiency in Acid Violet 109 (AV 109) oxidation. Under optimal conditions (pH 4, dye concentration 30 mg L-1, hydrogen peroxide concentration 0.1 mM), 72.4 and 88.9% of dye was oxidized using HRP-CLEAs in a batch and packed bed reactor, respectively. Higher stability was apparent in the packed bed reactor compared to the batch reactor where the mechanical instability of aggregates under constant agitation has been proved. After seven consecutive cycles the retained activity was 60 and 20% in the ...recycled packed bed and batch reactor, respectively. The feasibility of HRP-CLEAs application in dye oxidation was confirmed by significant dye oxidation percentage and reduction of the toxicity in the samples collected after enzymatic treatment in the recycled packed bed reactor, using brine shrimp, Artemia salina.
Keywords:
Armoracia rusticana / Bioremediation / Biotechnology / Green chemistry / ImmobilizationSource:
Clean-Soil Air Water, 2016, 44, 7, 891-900Publisher:
- Wiley, Hoboken
Funding / projects:
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Study of the Synthesis, Structure and Activity of Natural and Synthetic Organic Compounds (RS-MESTD-Basic Research (BR or ON)-172013)
- Electrochemical synthesis and characterization of nanostructured functional materials for application in new technologies (RS-MESTD-Basic Research (BR or ON)-172046)
DOI: 10.1002/clen.201500766
ISSN: 1863-0650
WoS: 000379942400018
Scopus: 2-s2.0-84968547018
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Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Šekuljica, Nataša AU - Prlainović, Nevena AU - Jakovetić, Sonja AU - Grbavčić, Sanja AU - Ognjanović, Nevena AU - Knežević-Jugović, Zorica AU - Mijin, Dušan PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3268 AB - Horseradish roots (Armoracia rusticana) fresh extract was the source of unpurified horseradish peroxidase (HRP) which was used for the immobilization in a form of cross-linked enzyme aggregates (CLEAs). Among three precipitants used, acetone appeared to be the best choice for the precipitation. Acetone with 2% of glutaraldehyde gave the recovery of 30.3% of the enzyme initial activity. Horseradish peroxidase immobilized in a form of cross-linked enzyme aggregates (HRP-CLEAs) showed remarkable efficiency in Acid Violet 109 (AV 109) oxidation. Under optimal conditions (pH 4, dye concentration 30 mg L-1, hydrogen peroxide concentration 0.1 mM), 72.4 and 88.9% of dye was oxidized using HRP-CLEAs in a batch and packed bed reactor, respectively. Higher stability was apparent in the packed bed reactor compared to the batch reactor where the mechanical instability of aggregates under constant agitation has been proved. After seven consecutive cycles the retained activity was 60 and 20% in the recycled packed bed and batch reactor, respectively. The feasibility of HRP-CLEAs application in dye oxidation was confirmed by significant dye oxidation percentage and reduction of the toxicity in the samples collected after enzymatic treatment in the recycled packed bed reactor, using brine shrimp, Artemia salina. PB - Wiley, Hoboken T2 - Clean-Soil Air Water T1 - Removal of Anthraquinone Dye by Cross-Linked Enzyme Aggregates From Fresh Horseradish Extract EP - 900 IS - 7 SP - 891 VL - 44 DO - 10.1002/clen.201500766 ER -
@article{ author = "Šekuljica, Nataša and Prlainović, Nevena and Jakovetić, Sonja and Grbavčić, Sanja and Ognjanović, Nevena and Knežević-Jugović, Zorica and Mijin, Dušan", year = "2016", abstract = "Horseradish roots (Armoracia rusticana) fresh extract was the source of unpurified horseradish peroxidase (HRP) which was used for the immobilization in a form of cross-linked enzyme aggregates (CLEAs). Among three precipitants used, acetone appeared to be the best choice for the precipitation. Acetone with 2% of glutaraldehyde gave the recovery of 30.3% of the enzyme initial activity. Horseradish peroxidase immobilized in a form of cross-linked enzyme aggregates (HRP-CLEAs) showed remarkable efficiency in Acid Violet 109 (AV 109) oxidation. Under optimal conditions (pH 4, dye concentration 30 mg L-1, hydrogen peroxide concentration 0.1 mM), 72.4 and 88.9% of dye was oxidized using HRP-CLEAs in a batch and packed bed reactor, respectively. Higher stability was apparent in the packed bed reactor compared to the batch reactor where the mechanical instability of aggregates under constant agitation has been proved. After seven consecutive cycles the retained activity was 60 and 20% in the recycled packed bed and batch reactor, respectively. The feasibility of HRP-CLEAs application in dye oxidation was confirmed by significant dye oxidation percentage and reduction of the toxicity in the samples collected after enzymatic treatment in the recycled packed bed reactor, using brine shrimp, Artemia salina.", publisher = "Wiley, Hoboken", journal = "Clean-Soil Air Water", title = "Removal of Anthraquinone Dye by Cross-Linked Enzyme Aggregates From Fresh Horseradish Extract", pages = "900-891", number = "7", volume = "44", doi = "10.1002/clen.201500766" }
Šekuljica, N., Prlainović, N., Jakovetić, S., Grbavčić, S., Ognjanović, N., Knežević-Jugović, Z.,& Mijin, D.. (2016). Removal of Anthraquinone Dye by Cross-Linked Enzyme Aggregates From Fresh Horseradish Extract. in Clean-Soil Air Water Wiley, Hoboken., 44(7), 891-900. https://doi.org/10.1002/clen.201500766
Šekuljica N, Prlainović N, Jakovetić S, Grbavčić S, Ognjanović N, Knežević-Jugović Z, Mijin D. Removal of Anthraquinone Dye by Cross-Linked Enzyme Aggregates From Fresh Horseradish Extract. in Clean-Soil Air Water. 2016;44(7):891-900. doi:10.1002/clen.201500766 .
Šekuljica, Nataša, Prlainović, Nevena, Jakovetić, Sonja, Grbavčić, Sanja, Ognjanović, Nevena, Knežević-Jugović, Zorica, Mijin, Dušan, "Removal of Anthraquinone Dye by Cross-Linked Enzyme Aggregates From Fresh Horseradish Extract" in Clean-Soil Air Water, 44, no. 7 (2016):891-900, https://doi.org/10.1002/clen.201500766 . .