Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode
Authorized Users Only
2016
Authors
Lović, JelenaTrišović, Nemanja
Antanasijević, Jelena
Nikolić, Nebojša
Stevanović, Sanja
Mijin, Dušan
Vuković, Dragan
Mladenović, Aleksandar R.
Petrović, Slobodan
Avramov-Ivić, Milka
Article (Published version)
Metadata
Show full item recordAbstract
Nonmedical use of sildenafil citrate (SC) requires new methods for drug determination in human serum and in tablets. SC as standard and in Sildena (R) tablets is determined by square wave voltammetry (SWV) in 0.1 M H2SO4 (harmful for human serum) at gold electrode in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.5,1) mu M and on cysteine (Cys) modified gold electrode (Au/Cys) in a range: (1 x 10(-3),1 x 10(-2), 0.05, 0.1) mu M. The presence of Cys causes two times larger peak currents and shifting of the incipient potential of the SC oxidation to 0.1 V in negative direction. A safe procedure for SC determination as standard and in Sildena (R) tablets spiked with human serum is developed by SWV at gold electrode in 0.05 M NaHCO3 (suitable for human liquids) in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.2, 03) mu M. Microscopic characterization of the surfaces morphology was also performed. The SC concentrations were checked by high performance liquid chromatography-ultraviolet spectroscopy (HPLC...-UV) showing that electrochemical method is more sensitive and can be used for the measurements of very low concentrations of the analyte.
Keywords:
Sildenafil citrate / Au/Cys electrode / Human serum analysis / Surface microscopic analysisSource:
Journal of Electroanalytical Chemistry, 2016, 782, 103-107Publisher:
- Elsevier Science Sa, Lausanne
Funding / projects:
- Study of the Synthesis, Structure and Activity of Natural and Synthetic Organic Compounds (RS-MESTD-Basic Research (BR or ON)-172013)
- New approach in designing materials for energy conversion and energy storage systems (RS-MESTD-Basic Research (BR or ON)-172060)
DOI: 10.1016/j.jelechem.2016.10.022
ISSN: 1572-6657
WoS: 000390510000015
Scopus: 2-s2.0-84992195164
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Lović, Jelena AU - Trišović, Nemanja AU - Antanasijević, Jelena AU - Nikolić, Nebojša AU - Stevanović, Sanja AU - Mijin, Dušan AU - Vuković, Dragan AU - Mladenović, Aleksandar R. AU - Petrović, Slobodan AU - Avramov-Ivić, Milka PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3312 AB - Nonmedical use of sildenafil citrate (SC) requires new methods for drug determination in human serum and in tablets. SC as standard and in Sildena (R) tablets is determined by square wave voltammetry (SWV) in 0.1 M H2SO4 (harmful for human serum) at gold electrode in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.5,1) mu M and on cysteine (Cys) modified gold electrode (Au/Cys) in a range: (1 x 10(-3),1 x 10(-2), 0.05, 0.1) mu M. The presence of Cys causes two times larger peak currents and shifting of the incipient potential of the SC oxidation to 0.1 V in negative direction. A safe procedure for SC determination as standard and in Sildena (R) tablets spiked with human serum is developed by SWV at gold electrode in 0.05 M NaHCO3 (suitable for human liquids) in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.2, 03) mu M. Microscopic characterization of the surfaces morphology was also performed. The SC concentrations were checked by high performance liquid chromatography-ultraviolet spectroscopy (HPLC-UV) showing that electrochemical method is more sensitive and can be used for the measurements of very low concentrations of the analyte. PB - Elsevier Science Sa, Lausanne T2 - Journal of Electroanalytical Chemistry T1 - Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode EP - 107 SP - 103 VL - 782 DO - 10.1016/j.jelechem.2016.10.022 ER -
@article{ author = "Lović, Jelena and Trišović, Nemanja and Antanasijević, Jelena and Nikolić, Nebojša and Stevanović, Sanja and Mijin, Dušan and Vuković, Dragan and Mladenović, Aleksandar R. and Petrović, Slobodan and Avramov-Ivić, Milka", year = "2016", abstract = "Nonmedical use of sildenafil citrate (SC) requires new methods for drug determination in human serum and in tablets. SC as standard and in Sildena (R) tablets is determined by square wave voltammetry (SWV) in 0.1 M H2SO4 (harmful for human serum) at gold electrode in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.5,1) mu M and on cysteine (Cys) modified gold electrode (Au/Cys) in a range: (1 x 10(-3),1 x 10(-2), 0.05, 0.1) mu M. The presence of Cys causes two times larger peak currents and shifting of the incipient potential of the SC oxidation to 0.1 V in negative direction. A safe procedure for SC determination as standard and in Sildena (R) tablets spiked with human serum is developed by SWV at gold electrode in 0.05 M NaHCO3 (suitable for human liquids) in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.2, 03) mu M. Microscopic characterization of the surfaces morphology was also performed. The SC concentrations were checked by high performance liquid chromatography-ultraviolet spectroscopy (HPLC-UV) showing that electrochemical method is more sensitive and can be used for the measurements of very low concentrations of the analyte.", publisher = "Elsevier Science Sa, Lausanne", journal = "Journal of Electroanalytical Chemistry", title = "Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode", pages = "107-103", volume = "782", doi = "10.1016/j.jelechem.2016.10.022" }
Lović, J., Trišović, N., Antanasijević, J., Nikolić, N., Stevanović, S., Mijin, D., Vuković, D., Mladenović, A. R., Petrović, S.,& Avramov-Ivić, M.. (2016). Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode. in Journal of Electroanalytical Chemistry Elsevier Science Sa, Lausanne., 782, 103-107. https://doi.org/10.1016/j.jelechem.2016.10.022
Lović J, Trišović N, Antanasijević J, Nikolić N, Stevanović S, Mijin D, Vuković D, Mladenović AR, Petrović S, Avramov-Ivić M. Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode. in Journal of Electroanalytical Chemistry. 2016;782:103-107. doi:10.1016/j.jelechem.2016.10.022 .
Lović, Jelena, Trišović, Nemanja, Antanasijević, Jelena, Nikolić, Nebojša, Stevanović, Sanja, Mijin, Dušan, Vuković, Dragan, Mladenović, Aleksandar R., Petrović, Slobodan, Avramov-Ivić, Milka, "Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode" in Journal of Electroanalytical Chemistry, 782 (2016):103-107, https://doi.org/10.1016/j.jelechem.2016.10.022 . .