Determination of anisomycin in tissues and serum by LC-MS/MS: application to pharmacokinetic and distribution studies in rats
Аутори
Tolić, LjiljanaGrujić, Svetlana
Mojović, Miloš
Jovanović, Miloš
Lubec, Gert
Bacić, Goran
Laušević, Mila
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A selective, sensitive and fast method for extraction, identification and quantification of multifunctional drug anisomycin in various tissues and serum, based on liquid chromatography-tandem mass spectrometry, was developed, optimized and validated. The method was validated according to the FDA guidelines and generally demonstrated good selectivity, accuracy, precision, and stability of the analyte. In the optimized method, high extraction efficiency was achieved for tested tissues (heart, brain, spleen, kidney, liver and femoral muscle) and serum. The obtained values of lower limits of quantification (LLOQ, 1.0-11.0 ng g(-1)) and limits of detection (0.3-3.3 ng g(-1)) indicated that the method was suitable for determination of trace levels of anisomycin in the complex matrices. The method was linear (R-2 gt = 0.990) in the tested concentration range (LLOQ-2500 ng g-1). The developed method was successfully applied in the first study on in vivo pharmacokinetics and distribution of an...isomycin in tissues and serum of Wistar albino rats following subcutaneous injection (150 mg kg(-1)). The peak concentration in most tissues was achieved within 3 h after injection. The highest anisomycin concentration was found in the brain and the lowest concentration was found in the serum.
Извор:
RSC Advances, 2016, 6, 95, 92479-92489Издавач:
- Royal Society of Chemistry
Финансирање / пројекти:
- Развој и примена метода и материјала за мониторинг нових загађујућих и токсичних органских материја и тешких метала (RS-MESTD-Basic Research (BR or ON)-172007)
- Биомаркери у неуродегенеративним и малигним процесима (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41005)
DOI: 10.1039/c6ra16083b
ISSN: 2046-2069
WoS: 000385389800042
Scopus: 2-s2.0-84989886017
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Tolić, Ljiljana AU - Grujić, Svetlana AU - Mojović, Miloš AU - Jovanović, Miloš AU - Lubec, Gert AU - Bacić, Goran AU - Laušević, Mila PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3367 AB - A selective, sensitive and fast method for extraction, identification and quantification of multifunctional drug anisomycin in various tissues and serum, based on liquid chromatography-tandem mass spectrometry, was developed, optimized and validated. The method was validated according to the FDA guidelines and generally demonstrated good selectivity, accuracy, precision, and stability of the analyte. In the optimized method, high extraction efficiency was achieved for tested tissues (heart, brain, spleen, kidney, liver and femoral muscle) and serum. The obtained values of lower limits of quantification (LLOQ, 1.0-11.0 ng g(-1)) and limits of detection (0.3-3.3 ng g(-1)) indicated that the method was suitable for determination of trace levels of anisomycin in the complex matrices. The method was linear (R-2 gt = 0.990) in the tested concentration range (LLOQ-2500 ng g-1). The developed method was successfully applied in the first study on in vivo pharmacokinetics and distribution of anisomycin in tissues and serum of Wistar albino rats following subcutaneous injection (150 mg kg(-1)). The peak concentration in most tissues was achieved within 3 h after injection. The highest anisomycin concentration was found in the brain and the lowest concentration was found in the serum. PB - Royal Society of Chemistry T2 - RSC Advances T1 - Determination of anisomycin in tissues and serum by LC-MS/MS: application to pharmacokinetic and distribution studies in rats EP - 92489 IS - 95 SP - 92479 VL - 6 DO - 10.1039/c6ra16083b ER -
@article{ author = "Tolić, Ljiljana and Grujić, Svetlana and Mojović, Miloš and Jovanović, Miloš and Lubec, Gert and Bacić, Goran and Laušević, Mila", year = "2016", abstract = "A selective, sensitive and fast method for extraction, identification and quantification of multifunctional drug anisomycin in various tissues and serum, based on liquid chromatography-tandem mass spectrometry, was developed, optimized and validated. The method was validated according to the FDA guidelines and generally demonstrated good selectivity, accuracy, precision, and stability of the analyte. In the optimized method, high extraction efficiency was achieved for tested tissues (heart, brain, spleen, kidney, liver and femoral muscle) and serum. The obtained values of lower limits of quantification (LLOQ, 1.0-11.0 ng g(-1)) and limits of detection (0.3-3.3 ng g(-1)) indicated that the method was suitable for determination of trace levels of anisomycin in the complex matrices. The method was linear (R-2 gt = 0.990) in the tested concentration range (LLOQ-2500 ng g-1). The developed method was successfully applied in the first study on in vivo pharmacokinetics and distribution of anisomycin in tissues and serum of Wistar albino rats following subcutaneous injection (150 mg kg(-1)). The peak concentration in most tissues was achieved within 3 h after injection. The highest anisomycin concentration was found in the brain and the lowest concentration was found in the serum.", publisher = "Royal Society of Chemistry", journal = "RSC Advances", title = "Determination of anisomycin in tissues and serum by LC-MS/MS: application to pharmacokinetic and distribution studies in rats", pages = "92489-92479", number = "95", volume = "6", doi = "10.1039/c6ra16083b" }
Tolić, L., Grujić, S., Mojović, M., Jovanović, M., Lubec, G., Bacić, G.,& Laušević, M.. (2016). Determination of anisomycin in tissues and serum by LC-MS/MS: application to pharmacokinetic and distribution studies in rats. in RSC Advances Royal Society of Chemistry., 6(95), 92479-92489. https://doi.org/10.1039/c6ra16083b
Tolić L, Grujić S, Mojović M, Jovanović M, Lubec G, Bacić G, Laušević M. Determination of anisomycin in tissues and serum by LC-MS/MS: application to pharmacokinetic and distribution studies in rats. in RSC Advances. 2016;6(95):92479-92489. doi:10.1039/c6ra16083b .
Tolić, Ljiljana, Grujić, Svetlana, Mojović, Miloš, Jovanović, Miloš, Lubec, Gert, Bacić, Goran, Laušević, Mila, "Determination of anisomycin in tissues and serum by LC-MS/MS: application to pharmacokinetic and distribution studies in rats" in RSC Advances, 6, no. 95 (2016):92479-92489, https://doi.org/10.1039/c6ra16083b . .