Приказ основних података о документу

dc.creatorBanjanac, Katarina
dc.creatorMihailović, Mladen
dc.creatorPrlainović, Nevena
dc.creatorStojanović, Marija
dc.creatorSimović, Milica
dc.creatorMarinković, Aleksandar
dc.creatorBezbradica, Dejan
dc.date.accessioned2021-03-10T13:09:56Z
dc.date.available2021-03-10T13:09:56Z
dc.date.issued2016
dc.identifier.issn0268-2575
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/3372
dc.description.abstractBACKGROUNDIn this work, fumed nano-silica (FNS) was chemically modified to amino (AFNS) and subsequently to cyanuric chloride (CCAFNS) modified silica and tested for the immobilization of lipase from Candida rugosa (CRL). The effects of the initial enzyme concentration, immobilization time and buffer ionic strength on immobilization were investigated in order to optimize utilization of the support and determine the mechanism of immobilization. The most active preparations were used to examine thermal and operational stability. RESULTSThe amount of immobilized enzyme increased with increasing enzyme concentration, achieving loadings of 121.3, 104.8 and 61.2mg per g of FNS, CCAFNS and AFNS, respectively. Lipase immobilized on CCAFNS carrier in 0.1molL(-1) buffer expressed the highest lipolytic activity (1320 IU g(-1) support), while more stable preparations were obtained in 1molL(-1) buffer. CONCLUSIONSuccessful modification of silica was confirmed with Fourier transform infrared spectroscopy and thermogravimetric analysis. Activity results showed that, depending on the support, immobilization was governed by different interactions. On FNS and AFNS immobilization was exclusively by adsorption, while on CCAFNS after the initial adsorption lipase molecules reoriented and amino groups of the enzyme formed a covalent bond with the chlorine atom of the modified carrier. Improved thermal and operational stability of lipase immobilized on CCAFNS in 1molL(-1) buffer led to the conclusion that electrostatic interactions have a great role in the immobilization.en
dc.publisherWiley-Blackwell, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172013/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45019/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Chemical Technology and Biotechnology
dc.subjectsilica nanoparticlesen
dc.subjectmodificationen
dc.subjectAPTMSen
dc.subjectcyanuric chlorideen
dc.subjectlipaseen
dc.subjectenzyme immobilizationen
dc.titleCyanuric chloride functionalized silica nanoparticles for covalent immobilization of lipaseen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage448
dc.citation.issue2
dc.citation.other91(2): 439-448
dc.citation.rankM21
dc.citation.spage439
dc.citation.volume91
dc.identifier.doi10.1002/jctb.4595
dc.identifier.scopus2-s2.0-84953839970
dc.identifier.wos000367951200019
dc.type.versionpublishedVersion


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Приказ основних података о документу