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dc.creatorSimović, Milica
dc.creatorĆorović, Marija
dc.creatorMihailović, Mladen
dc.creatorBanjanac, Katarina
dc.creatorMilivojević, Ana
dc.creatorVeličković, Dušan
dc.creatorBezbradica, Dejan
dc.date.accessioned2021-03-10T13:10:04Z
dc.date.available2021-03-10T13:10:04Z
dc.date.issued2016
dc.identifier.issn0958-6946
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/3374
dc.description.abstractThe goal of this study was to establish an efficient immobilisation protocol for beta-galactosidase from Aspergillus oryzae onto the polystyrenic macroporous resin Purolite (R) A-109 for better utilisation of its transglactosylation activity and application in galacto-oligosaccharide (GOS) synthesis. This was achieved by improving simple ionic adsorption by carboxyl group activation on the enzyme surface with carbodiimide, enabling covalent immobilisation. This yielded significantly increased operational stability, assayed as GOS synthesis, in a batch reactor, and even more prominently, in a fluidised bed reactor (73% activity retained after 10 cycles). The immobilised enzyme showed two very beneficial advantages over the free enzyme for future applications: higher affinity towards catalysing transgalactosylation than towards hydrolysis and shift of pH optimum towards more acidic conditions. GOS synthesis performed under the optimum conditions obtained (400 g L-1 lactose, pH 4.5, 50 degrees C) yielded 87 g L-1 and 100 g L-1 for batch and fluidised bed reactors, respectively.en
dc.publisherElsevier Sci Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.rightsrestrictedAccess
dc.sourceInternational Dairy Journal
dc.titleGalacto-oligosaccharide synthesis using chemically modified beta-galactosidase from Aspergillus oryzae immobilised onto macroporous amino resinen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage57
dc.citation.other54: 50-57
dc.citation.rankM22
dc.citation.spage50
dc.citation.volume54
dc.identifier.doi10.1016/j.idairyj.2015.10.002
dc.identifier.rcubconv_4877
dc.identifier.scopus2-s2.0-84949845593
dc.identifier.wos000368021400007
dc.type.versionpublishedVersion


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