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Single step recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction
dc.creator | Antov, Mirjana | |
dc.creator | Ivetić, Darjana Z. | |
dc.creator | Knežević-Jugović, Zorica | |
dc.date.accessioned | 2021-03-10T13:14:15Z | |
dc.date.available | 2021-03-10T13:14:15Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0149-6395 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3438 | |
dc.description.abstract | Recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction was studied with maximal possible crude enzyme loads. In polyethylene glycol/dextran and polyethylene glycol/salt systems the influences of molecular weight and concentration of polyethylene glycol, phase-forming salt and phase volume ratio were evaluated. Lipase partition coefficient 9 followed by the top phase yield 95.7% and purification factor 3.4 were achieved in 15% (w w(-1)) polyethylene glycol 4000/15% (w w(-1)) KH2PO4/70% (w w(-1)) crude enzyme. Efficient single-step recovery of lipase followed by partial enzyme purification indicated possible integration of production and primary bioseparation step by aqueous two-phase extraction. By varying phase volume ratio, the concentration of phosphate was reduced without decrease in lipase recovery. | en |
dc.publisher | Taylor & Francis Inc, Philadelphia | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS// | |
dc.rights | restrictedAccess | |
dc.source | Separation Science and Technology | |
dc.subject | bioseparation | en |
dc.subject | Aqueous two-phase extraction | en |
dc.subject | partitioning | en |
dc.subject | recovery | en |
dc.subject | lipase | en |
dc.title | Single step recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 628 | |
dc.citation.issue | 4 | |
dc.citation.other | 51(4): 622-628 | |
dc.citation.rank | M23 | |
dc.citation.spage | 622 | |
dc.citation.volume | 51 | |
dc.identifier.doi | 10.1080/01496395.2015.1117106 | |
dc.identifier.scopus | 2-s2.0-84958779524 | |
dc.identifier.wos | 000372914000007 | |
dc.type.version | publishedVersion |
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