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dc.creatorŠekuljica, Nataša
dc.creatorPrlainović, Nevena
dc.creatorJovanović, Jelena
dc.creatorStefanović, Andrea
dc.creatorĐokić, Veljko
dc.creatorMijin, Dušan
dc.creatorKnežević-Jugović, Zorica
dc.date.accessioned2021-03-10T13:16:06Z
dc.date.available2021-03-10T13:16:06Z
dc.date.issued2016
dc.identifier.issn1615-7591
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/3466
dc.description.abstractKaolin showed as a very perspective carrier for the enzyme immobilization and it was used for the adsorption of horseradish peroxidase (HRP). The effects of the enzyme concentration and pH on the immobilization efficiency were studied in the reaction with pyrogallol and anthraquinone dye C.I. Acid Violet 109 (AV 109). In addition, Fourier transform infrared spectroscopy, scanning electron microscopy and analysis by Brunauer-EmmettTeller were performed for kaolin, thermally activated kaolin and the immobilized enzyme. It has been shown that 0.1 IU of HRP-kaolin decolorized 87 % of dye solution, under the optimal conditions (pH 5.0, temperature 24 degrees C, dye concentration 40 mg/L and 0.2 mM of H2O2) within 40 min. The immobilized HRP decolorization follows the Ping Pong Bi-Bi mechanism with dead-end inhibition by the dye. The biocatalyst retained 35 +/- 0.9 % of the initial activity after seven cycles of reuse in the decolorization reaction of AV 109 under optimal conditions in a batch reactor. The obtained kinetic parameters and reusability study confirmed improvement in performances of k-HRP compared to free, indicating that k-HRP has a great potential for environmental purposes.en
dc.publisherSpringer, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172013/RS//
dc.rightsrestrictedAccess
dc.sourceBioprocess and Biosystems Engineering
dc.subjectHorseradish peroxidaseen
dc.subjectKaolinen
dc.subjectAdsorptionen
dc.subjectKinetic parametersen
dc.subjectWastewater treatmenten
dc.titleImmobilization of horseradish peroxidase onto kaolinen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage472
dc.citation.issue3
dc.citation.other39(3): 461-472
dc.citation.rankM22
dc.citation.spage461
dc.citation.volume39
dc.identifier.doi10.1007/s00449-015-1529-x
dc.identifier.pmid26747440
dc.identifier.rcubconv_4926
dc.identifier.scopus2-s2.0-84960101239
dc.identifier.wos000371071100010
dc.type.versionpublishedVersion


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