In-vitro visualization of primary tumor cells using up-conversion nanophosphors
2017
Аутори
Mančić, LidijaĐukić-Vuković, Aleksandra
Mojović, Ljiljana
Rabasović, Mihailo D.
Krmpot, Aleksandar
Dinić, Ivana
Costa, Antonio Mario Leal Martins
Milošević, Olivera
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
For such purpose, Ln-UCNPs should have specific morphological characteristics and efficient luminescence response. In this work, a biocompatible and water dispersible NaYF4:Yb,Er"PLGA nanoparticles synthesized using a one-step hydrothermal synthesis were tested as fluorescent bio-labels of primary cell cultures obtained after passage of head and neck squamous carcinoma cells (HNSCC). Structural, morphological and optical properties of particles were obtained using X-ray powder diffraction (XRPD), field emission scanning and transmission electron microscopy (FESEM/TEM), energy dispersive X-ray (EDX), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy. The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P63/m) phase in spherical and irregularly shaped nanoparticles, respectively. Moreover, preservation of the PLGA ligands at the particles surface facilitates their interactions with the cell membrane and provides permeation into cells. To asses a bi...ological safety of their use, viability of human gingival fibroblasts (HFG) was additionally evaluated by a colorimetric MTT assay.
Кључне речи:
visualization / tumor cells / up-conversion / nanophosphors / X-ray powder diffraction (XRPD) / energy dispersive X-ray (EDX) / Fourier transform infrared spectroscopy (FTIR) / photoluminescence spectroscopy (PL) / FESEM/TEMИзвор:
Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Applica, 2017, 59-59Издавач:
- Belgrade : Serbian Ceramic Society
Финансирање / пројекти:
- Генерисање и карактеризација нанофотонских функционалних структура у биомедицини и информатици (RS-45016)
- Рационални дизајн и синтеза биолошки активних и координационих једињења и функционалних материјала, релевантних у (био)нанотехнологији (RS-172035)
Институција/група
Tehnološko-metalurški fakultetTY - CONF AU - Mančić, Lidija AU - Đukić-Vuković, Aleksandra AU - Mojović, Ljiljana AU - Rabasović, Mihailo D. AU - Krmpot, Aleksandar AU - Dinić, Ivana AU - Costa, Antonio Mario Leal Martins AU - Milošević, Olivera PY - 2017 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3488 AB - For such purpose, Ln-UCNPs should have specific morphological characteristics and efficient luminescence response. In this work, a biocompatible and water dispersible NaYF4:Yb,Er"PLGA nanoparticles synthesized using a one-step hydrothermal synthesis were tested as fluorescent bio-labels of primary cell cultures obtained after passage of head and neck squamous carcinoma cells (HNSCC). Structural, morphological and optical properties of particles were obtained using X-ray powder diffraction (XRPD), field emission scanning and transmission electron microscopy (FESEM/TEM), energy dispersive X-ray (EDX), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy. The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P63/m) phase in spherical and irregularly shaped nanoparticles, respectively. Moreover, preservation of the PLGA ligands at the particles surface facilitates their interactions with the cell membrane and provides permeation into cells. To asses a biological safety of their use, viability of human gingival fibroblasts (HFG) was additionally evaluated by a colorimetric MTT assay. PB - Belgrade : Serbian Ceramic Society C3 - Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Applica T1 - In-vitro visualization of primary tumor cells using up-conversion nanophosphors EP - 59 SP - 59 UR - https://hdl.handle.net/21.15107/rcub_technorep_3488 ER -
@conference{ author = "Mančić, Lidija and Đukić-Vuković, Aleksandra and Mojović, Ljiljana and Rabasović, Mihailo D. and Krmpot, Aleksandar and Dinić, Ivana and Costa, Antonio Mario Leal Martins and Milošević, Olivera", year = "2017", abstract = "For such purpose, Ln-UCNPs should have specific morphological characteristics and efficient luminescence response. In this work, a biocompatible and water dispersible NaYF4:Yb,Er"PLGA nanoparticles synthesized using a one-step hydrothermal synthesis were tested as fluorescent bio-labels of primary cell cultures obtained after passage of head and neck squamous carcinoma cells (HNSCC). Structural, morphological and optical properties of particles were obtained using X-ray powder diffraction (XRPD), field emission scanning and transmission electron microscopy (FESEM/TEM), energy dispersive X-ray (EDX), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy. The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P63/m) phase in spherical and irregularly shaped nanoparticles, respectively. Moreover, preservation of the PLGA ligands at the particles surface facilitates their interactions with the cell membrane and provides permeation into cells. To asses a biological safety of their use, viability of human gingival fibroblasts (HFG) was additionally evaluated by a colorimetric MTT assay.", publisher = "Belgrade : Serbian Ceramic Society", journal = "Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Applica", title = "In-vitro visualization of primary tumor cells using up-conversion nanophosphors", pages = "59-59", url = "https://hdl.handle.net/21.15107/rcub_technorep_3488" }
Mančić, L., Đukić-Vuković, A., Mojović, L., Rabasović, M. D., Krmpot, A., Dinić, I., Costa, A. M. L. M.,& Milošević, O.. (2017). In-vitro visualization of primary tumor cells using up-conversion nanophosphors. in Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Applica Belgrade : Serbian Ceramic Society., 59-59. https://hdl.handle.net/21.15107/rcub_technorep_3488
Mančić L, Đukić-Vuković A, Mojović L, Rabasović MD, Krmpot A, Dinić I, Costa AMLM, Milošević O. In-vitro visualization of primary tumor cells using up-conversion nanophosphors. in Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Applica. 2017;:59-59. https://hdl.handle.net/21.15107/rcub_technorep_3488 .
Mančić, Lidija, Đukić-Vuković, Aleksandra, Mojović, Ljiljana, Rabasović, Mihailo D., Krmpot, Aleksandar, Dinić, Ivana, Costa, Antonio Mario Leal Martins, Milošević, Olivera, "In-vitro visualization of primary tumor cells using up-conversion nanophosphors" in Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Applica (2017):59-59, https://hdl.handle.net/21.15107/rcub_technorep_3488 .