Приказ основних података о документу

dc.creatorŽuža, Milena
dc.creatorMilašinović, Nikola
dc.creatorJonović, Marko M.
dc.creatorJovanović, Jelena
dc.creatorKalagasidis Krušić, Melina
dc.creatorBugarski, Branko
dc.creatorKnežević-Jugović, Zorica
dc.date.accessioned2021-03-10T13:21:07Z
dc.date.available2021-03-10T13:21:07Z
dc.date.issued2017
dc.identifier.issn1615-7591
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/3544
dc.description.abstractIn this study, alcalase (protease from Bacillus licheniformis) immobilization by adsorption, enzyme crosslinking and covalent enzyme binding to activated chitosan microbeads were examined. The biocatalysts highest activity was obtained by covalent immobilization of alcalase onto a solid support. The alcalase covalent immobilization onto different types of chitosan beads obtained by inverse emulsion technique and electrostatic extrusion was studied. Parameters examined under different conditions were beads diameter, enzyme loading, enzyme capacity yield, and biocatalyst activity. The highest activity and enzyme loading of 23.6 IU/mg protein and 340.2 mg/g, respectively, were achieved by the enzyme immobilized onto chitosan microbeads obtained by the electrostatic extrusion technique. FT-IR analysis was used to confirm formation of alcalase-chitosan conjugates. The activity of optimally produced alcalase-chitosan microbeads was then verified in the industrially feasible reaction systems of egg white and soy protein hydrolysis. The high degree of hydrolysis of 29.85 +/- 0.967% after 180 min and five successive reuses obtained under real conditions (50 A degrees C, pH 8) verified the covalently bound alcalase to chitosan beads a promising candidate for use in industrial egg white protein hydrolysis process.en
dc.publisherSpringer, New York
dc.relationMinistry of Education, Science and Technological Development of Serbia
dc.relationMinistry of Interior of the Republic of Serbia [242/16-4-2014]
dc.rightsrestrictedAccess
dc.sourceBioprocess and Biosystems Engineering
dc.subjectAlcalaseen
dc.subjectChitosan microbeadsen
dc.subjectEmulsion techniqueen
dc.subjectElectrostatic extrusionen
dc.subjectImmobilizationen
dc.titleDesign and characterization of alcalase-chitosan conjugates as potential biocatalystsen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage1723
dc.citation.issue11
dc.citation.other40(11): 1713-1723
dc.citation.rankM22
dc.citation.spage1713
dc.citation.volume40
dc.identifier.doi10.1007/s00449-017-1826-7
dc.identifier.pmid28801712
dc.identifier.scopus2-s2.0-85027300125
dc.identifier.wos000411189800013
dc.type.versionpublishedVersion


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Приказ основних података о документу