Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode
2017
Аутори
Lović, JelenaStevanović, Sanja
Nikolić, Nebojša
Petrović, Slobodan
Vuković, Dragan
Prlainović, Nevena
Mijin, Dušan
Avramov-Ivić, Milka
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum.
Кључне речи:
Biosensors / Immobilization / Enzyme catalysis / Self-assemblyИзвор:
International Journal of Electrochemical Science, 2017, 12, 7, 5806-5817Издавач:
- Electrochemical Science Group, Beograd
Финансирање / пројекти:
- Проучавање синтезе, структуре и активности органских једињења природног и синтетског порекла (RS-172013)
- Нов приступ дизајнирању материјала за конверзију и складиштење енергије (RS-172060)
DOI: 10.20964/2017.07.65
ISSN: 1452-3981
WoS: 000406090700001
Scopus: 2-s2.0-85023746929
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Lović, Jelena AU - Stevanović, Sanja AU - Nikolić, Nebojša AU - Petrović, Slobodan AU - Vuković, Dragan AU - Prlainović, Nevena AU - Mijin, Dušan AU - Avramov-Ivić, Milka PY - 2017 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3570 AB - The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum. PB - Electrochemical Science Group, Beograd T2 - International Journal of Electrochemical Science T1 - Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode EP - 5817 IS - 7 SP - 5806 VL - 12 DO - 10.20964/2017.07.65 ER -
@article{ author = "Lović, Jelena and Stevanović, Sanja and Nikolić, Nebojša and Petrović, Slobodan and Vuković, Dragan and Prlainović, Nevena and Mijin, Dušan and Avramov-Ivić, Milka", year = "2017", abstract = "The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum.", publisher = "Electrochemical Science Group, Beograd", journal = "International Journal of Electrochemical Science", title = "Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode", pages = "5817-5806", number = "7", volume = "12", doi = "10.20964/2017.07.65" }
Lović, J., Stevanović, S., Nikolić, N., Petrović, S., Vuković, D., Prlainović, N., Mijin, D.,& Avramov-Ivić, M.. (2017). Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode. in International Journal of Electrochemical Science Electrochemical Science Group, Beograd., 12(7), 5806-5817. https://doi.org/10.20964/2017.07.65
Lović J, Stevanović S, Nikolić N, Petrović S, Vuković D, Prlainović N, Mijin D, Avramov-Ivić M. Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode. in International Journal of Electrochemical Science. 2017;12(7):5806-5817. doi:10.20964/2017.07.65 .
Lović, Jelena, Stevanović, Sanja, Nikolić, Nebojša, Petrović, Slobodan, Vuković, Dragan, Prlainović, Nevena, Mijin, Dušan, Avramov-Ivić, Milka, "Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode" in International Journal of Electrochemical Science, 12, no. 7 (2017):5806-5817, https://doi.org/10.20964/2017.07.65 . .