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Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate
dc.creator | Miljković, Miona | |
dc.creator | Davidović, Slađana | |
dc.creator | Kralj, Slavko | |
dc.creator | Šiler-Marinković, Slavica | |
dc.creator | Rajilić-Stojanović, Mirjana | |
dc.creator | Dimitrijević-Branković, Suzana | |
dc.date.accessioned | 2021-03-10T13:26:57Z | |
dc.date.available | 2021-03-10T13:26:57Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0367-598X | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3632 | |
dc.description.abstract | The production of dextransucrase (DS) by Leuconostoc mesenteroides T3, novel isolate from water kefir grain, was studied and optimized. Bacterial supernatant reached activity of 3.1 U/ml when the culture was grown at 23 degrees C and under static culture condition using classical Tsuchiya medium for DS production. The increase of sucrose concentration to 7% led to an increase of DS activity by 52% compared to the control. Medium with 2% beef extract and 1% yeast extract resulted in 4.52 U/ml, which was 47% higher than in the control (with 2% yeast extract). Finally, the increase of K2HPO4 concentration from 2 to 3% resulted in the increased enzyme activity by 28%. Enzyme purified by polyethylene glycol 400 fractionation displayed maximum activity at 30 degrees C and pH 5.4. Zymogram analysis confirmed the presence of DS of approximately 180 kDa. The addition of divalent cations Ca2+, Mg2+, Fe2+ and Co2+ led to a minor increase of DS activity, while the addition of Mn2+ was the most prominent with 73% increase. These findings classify dextransucrase from Leuconostoc mesenteroides T3 as promising candidate for production of dextran, which has numerous applications in various industries. | en |
dc.publisher | Savez hemijskih inženjera, Beograd | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31035/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Hemijska industrija | |
dc.subject | lactic acid bacteria | en |
dc.subject | Leuconostoc mesenteroides | en |
dc.subject | dextransucrase | en |
dc.subject | dextran | en |
dc.title | Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dc.citation.epage | 360 | |
dc.citation.issue | 4 | |
dc.citation.other | 71(4): 351-360 | |
dc.citation.rank | M23 | |
dc.citation.spage | 351 | |
dc.citation.volume | 71 | |
dc.identifier.doi | 10.2298/HEMIND160421046M | |
dc.identifier.fulltext | http://TechnoRep.tmf.bg.ac.rs/bitstream/id/9600/0367-598X1600046M.pdf | |
dc.identifier.scopus | 2-s2.0-85030211936 | |
dc.identifier.wos | 000413180100009 | |
dc.type.version | publishedVersion |