Batch and semicontinuous production of L-ascorbyl oleate catalyzed by CALB immobilized onto Purolite (R) MN102
Само за регистроване кориснике
2017
Аутори
Ćorović, MarijaMilivojević, Ana
Carević, Milica
Banjanac, Katarina
Jakovetić Tanasković, Sonja
Bezbradica, Dejan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
L-Ascorbyl oleate is recognized as efficient liposoluble antioxidant and biosurfactant which can be synthesized by using immobilized lipases as catalysts in organic media. However, development of economical production process is still a challenge, particularly in (semi)continuous systems. Hereby, we present application of novel immobilized lipase preparation, lipase B from Candida antarctica hydrophobically adsorbed onto styrene divinylbenzene based support, for the synthesis of L-ascorbyl oleate in batch bioreactor (BB) and fluidized bed bioreactor (FBB) with recirculation of reaction medium. Reaction kinetics was described by Ping Pong bi bi mechanism with low acyl donor inhibition. Considerably higher reaction rates (similar to 40%) were accomplished in FBB. Hydrodynamic study revealed that reaction is kinetically controlled and unhindered external and internal mass transfer was enabled in both systems. However, much higher shear stress occurred in BB (10.46 Pa) comparing to FBB (5 ...x 10(-2) Pa). After 12 consecutive reaction cycles lasting 24h, 23.13 g of product per gram of biocatalyst was synthesized in a FBB. Within this study, usage of new immobilized enzyme in semicontinuous system enabled high reaction rates and satisfying operational stability in the synthesis of L-ascorbyl oleate, offering good prospect for further process development.
Кључне речи:
Immobilized lipase / Ascorbyl oleate / Esterification / Fluidized bed bioreactor / Kinetics / HydrodynamicsИзвор:
Chemical Engineering Research & Design, 2017, 126, 161-171Издавач:
- Elsevier, Amsterdam
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.1016/j.cherd.2017.08.021
ISSN: 0263-8762
WoS: 000413387600015
Scopus: 2-s2.0-85031115544
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Ćorović, Marija AU - Milivojević, Ana AU - Carević, Milica AU - Banjanac, Katarina AU - Jakovetić Tanasković, Sonja AU - Bezbradica, Dejan PY - 2017 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3650 AB - L-Ascorbyl oleate is recognized as efficient liposoluble antioxidant and biosurfactant which can be synthesized by using immobilized lipases as catalysts in organic media. However, development of economical production process is still a challenge, particularly in (semi)continuous systems. Hereby, we present application of novel immobilized lipase preparation, lipase B from Candida antarctica hydrophobically adsorbed onto styrene divinylbenzene based support, for the synthesis of L-ascorbyl oleate in batch bioreactor (BB) and fluidized bed bioreactor (FBB) with recirculation of reaction medium. Reaction kinetics was described by Ping Pong bi bi mechanism with low acyl donor inhibition. Considerably higher reaction rates (similar to 40%) were accomplished in FBB. Hydrodynamic study revealed that reaction is kinetically controlled and unhindered external and internal mass transfer was enabled in both systems. However, much higher shear stress occurred in BB (10.46 Pa) comparing to FBB (5 x 10(-2) Pa). After 12 consecutive reaction cycles lasting 24h, 23.13 g of product per gram of biocatalyst was synthesized in a FBB. Within this study, usage of new immobilized enzyme in semicontinuous system enabled high reaction rates and satisfying operational stability in the synthesis of L-ascorbyl oleate, offering good prospect for further process development. PB - Elsevier, Amsterdam T2 - Chemical Engineering Research & Design T1 - Batch and semicontinuous production of L-ascorbyl oleate catalyzed by CALB immobilized onto Purolite (R) MN102 EP - 171 SP - 161 VL - 126 DO - 10.1016/j.cherd.2017.08.021 ER -
@article{ author = "Ćorović, Marija and Milivojević, Ana and Carević, Milica and Banjanac, Katarina and Jakovetić Tanasković, Sonja and Bezbradica, Dejan", year = "2017", abstract = "L-Ascorbyl oleate is recognized as efficient liposoluble antioxidant and biosurfactant which can be synthesized by using immobilized lipases as catalysts in organic media. However, development of economical production process is still a challenge, particularly in (semi)continuous systems. Hereby, we present application of novel immobilized lipase preparation, lipase B from Candida antarctica hydrophobically adsorbed onto styrene divinylbenzene based support, for the synthesis of L-ascorbyl oleate in batch bioreactor (BB) and fluidized bed bioreactor (FBB) with recirculation of reaction medium. Reaction kinetics was described by Ping Pong bi bi mechanism with low acyl donor inhibition. Considerably higher reaction rates (similar to 40%) were accomplished in FBB. Hydrodynamic study revealed that reaction is kinetically controlled and unhindered external and internal mass transfer was enabled in both systems. However, much higher shear stress occurred in BB (10.46 Pa) comparing to FBB (5 x 10(-2) Pa). After 12 consecutive reaction cycles lasting 24h, 23.13 g of product per gram of biocatalyst was synthesized in a FBB. Within this study, usage of new immobilized enzyme in semicontinuous system enabled high reaction rates and satisfying operational stability in the synthesis of L-ascorbyl oleate, offering good prospect for further process development.", publisher = "Elsevier, Amsterdam", journal = "Chemical Engineering Research & Design", title = "Batch and semicontinuous production of L-ascorbyl oleate catalyzed by CALB immobilized onto Purolite (R) MN102", pages = "171-161", volume = "126", doi = "10.1016/j.cherd.2017.08.021" }
Ćorović, M., Milivojević, A., Carević, M., Banjanac, K., Jakovetić Tanasković, S.,& Bezbradica, D.. (2017). Batch and semicontinuous production of L-ascorbyl oleate catalyzed by CALB immobilized onto Purolite (R) MN102. in Chemical Engineering Research & Design Elsevier, Amsterdam., 126, 161-171. https://doi.org/10.1016/j.cherd.2017.08.021
Ćorović M, Milivojević A, Carević M, Banjanac K, Jakovetić Tanasković S, Bezbradica D. Batch and semicontinuous production of L-ascorbyl oleate catalyzed by CALB immobilized onto Purolite (R) MN102. in Chemical Engineering Research & Design. 2017;126:161-171. doi:10.1016/j.cherd.2017.08.021 .
Ćorović, Marija, Milivojević, Ana, Carević, Milica, Banjanac, Katarina, Jakovetić Tanasković, Sonja, Bezbradica, Dejan, "Batch and semicontinuous production of L-ascorbyl oleate catalyzed by CALB immobilized onto Purolite (R) MN102" in Chemical Engineering Research & Design, 126 (2017):161-171, https://doi.org/10.1016/j.cherd.2017.08.021 . .