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dc.creatorĆorović, Marija
dc.creatorMilivojević, Ana
dc.creatorCarević, Milica
dc.creatorBanjanac, Katarina
dc.creatorJakovetić-Tanasković, Sonja
dc.creatorBezbradica, Dejan
dc.date.accessioned2021-03-10T13:28:07Z
dc.date.available2021-03-10T13:28:07Z
dc.date.issued2017
dc.identifier.issn0263-8762
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/3650
dc.description.abstractL-Ascorbyl oleate is recognized as efficient liposoluble antioxidant and biosurfactant which can be synthesized by using immobilized lipases as catalysts in organic media. However, development of economical production process is still a challenge, particularly in (semi)continuous systems. Hereby, we present application of novel immobilized lipase preparation, lipase B from Candida antarctica hydrophobically adsorbed onto styrene divinylbenzene based support, for the synthesis of L-ascorbyl oleate in batch bioreactor (BB) and fluidized bed bioreactor (FBB) with recirculation of reaction medium. Reaction kinetics was described by Ping Pong bi bi mechanism with low acyl donor inhibition. Considerably higher reaction rates (similar to 40%) were accomplished in FBB. Hydrodynamic study revealed that reaction is kinetically controlled and unhindered external and internal mass transfer was enabled in both systems. However, much higher shear stress occurred in BB (10.46 Pa) comparing to FBB (5 x 10(-2) Pa). After 12 consecutive reaction cycles lasting 24h, 23.13 g of product per gram of biocatalyst was synthesized in a FBB. Within this study, usage of new immobilized enzyme in semicontinuous system enabled high reaction rates and satisfying operational stability in the synthesis of L-ascorbyl oleate, offering good prospect for further process development.en
dc.publisherElsevier, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.rightsrestrictedAccess
dc.sourceChemical Engineering Research & Design
dc.subjectImmobilized lipaseen
dc.subjectAscorbyl oleateen
dc.subjectEsterificationen
dc.subjectFluidized bed bioreactoren
dc.subjectKineticsen
dc.subjectHydrodynamicsen
dc.titleBatch and semicontinuous production of L-ascorbyl oleate catalyzed by CALB immobilized onto Purolite (R) MN102en
dc.typearticle
dc.rights.licenseARR
dc.citation.epage171
dc.citation.other126: 161-171
dc.citation.rankM22
dc.citation.spage161
dc.citation.volume126
dc.identifier.doi10.1016/j.cherd.2017.08.021
dc.identifier.rcubconv_5429
dc.identifier.scopus2-s2.0-85031115544
dc.identifier.wos000413387600015
dc.type.versionpublishedVersion


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