Приказ основних података о документу

dc.creatorPajić-Lijaković, Ivana
dc.creatorMilivojević, Milan
dc.creatorLević, Steva
dc.creatorTrifković, Kata T.
dc.creatorStevanović-Dajić, Zora
dc.creatorRadosević, Radenko
dc.creatorNedović, Viktor
dc.creatorBugarski, Branko
dc.date.accessioned2021-03-10T13:29:13Z
dc.date.available2021-03-10T13:29:13Z
dc.date.issued2017
dc.identifier.issn1359-5113
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/3666
dc.description.abstractMicroenvironmentally restricted yeast cell growth within Ca-alginate beads with and without entrapped gas bubbles was considered based on experimental data. Cell growth dynamics was described by (1) the dimensionless cell number density as a function of the cell growth time and (2) the cell distribution per bead cross sections. One of the key control parameters for bioprocess optimization is the matrix resistance stress generated during immobilized cell expansion. The dynamics of the increase in matrix stress was described theoretically based on a multi-scale mathematical model. In order to estimate and reduce the accumulation of matrix stress we considered repeated stress relaxation cycles in separate rheological experiments without immobilized cells. The results revealed that the increase in resistance stress within the Ca-alginate matrix was significant (similar to 7 kPa) after 10 repeated cycles, even under a low compression strain of 2% per cycle. The stress could be reduced by using the Ca-alginate matrix with entrapped gas bubbles. The final cell concentration within the beads with entrapped bubbles was 3.3 times higher in comparison with the beads without bubbles. The bubbles could locally amortize the compression effects within the surrounding cell clusters.en
dc.publisherElsevier Sci Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/316004/EU//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46001/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.rightsrestrictedAccess
dc.sourceProcess Biochemistry
dc.subjectCa-alginate bead with entrapped gas bubblesen
dc.subjectYeasten
dc.subjectMatrix resistance stress generationen
dc.subjectMatrix rheological propertiesen
dc.subjectModelingen
dc.subjectMicroenvironmentally restricted cell growthen
dc.titleMatrix resistance stress: A key parameter for immobilized cell growth regulationen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage43
dc.citation.other52: 30-43
dc.citation.rankM22
dc.citation.spage30
dc.citation.volume52
dc.identifier.doi10.1016/j.procbio.2016.10.017
dc.identifier.scopus2-s2.0-85002756249
dc.identifier.wos000392774600004
dc.type.versionpublishedVersion


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Приказ основних података о документу