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dc.creatorTrivanović, Drenka
dc.creatorDrvenica, Ivana
dc.creatorKukolj, Tamara
dc.creatorObradović, Hristina
dc.creatorOkić-Đorđević, Ivana
dc.creatorMojsilović, Slavko
dc.creatorKrstić, Jelena
dc.creatorBugarski, Branko
dc.creatorJauković, Aleksandra
dc.creatorBugarski, Diana
dc.date.accessioned2021-03-10T13:35:23Z
dc.date.available2021-03-10T13:35:23Z
dc.date.issued2018
dc.identifier.issn2169-1401
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/3761
dc.description.abstractAdipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.en
dc.publisherTaylor & Francis Ltd, Abingdon
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175062/RS//
dc.relationfellowship "Start up for Science, Serbia" 2015/2016 (Company Philip Morris)
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.sourceArtificial Cells Nanomedicine and Biotechnology
dc.subjectAdipose stem cellsen
dc.subjectextracellular matrixen
dc.subjectdifferentiationen
dc.subjectcytoskeletonen
dc.subjectmitochondriaen
dc.subjectSIRT1en
dc.titleAdipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cellsen
dc.typearticle
dc.rights.licenseBY-NC
dc.citation.epageS382
dc.citation.other46: S370-S382
dc.citation.rankM21
dc.citation.spageS370
dc.citation.volume46
dc.identifier.doi10.1080/21691401.2018.1494183
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/1584/3758.pdf
dc.identifier.pmid30198336
dc.identifier.scopus2-s2.0-85053249346
dc.identifier.wos000460141900036
dc.type.versionpublishedVersion


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