Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode
2018
Аутори
Lović, JelenaStevanović, Sanja
Anđelković, Boban D.
Petrović, Slobodan
Vuković, Dragan
Prlainović, Nevena
Mijin, Dušan
Nikolić, Nebojša
Avramov-Ivić, Milka
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Glucose biosensor containing cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is constructed and already electrochemically tested. Now, the electrochemical behavior of biosensor in human serum is further investigated and supported by morphological characterization of layers for the first time. The morphology and microstructure of layers was examined by Fourier transformed infra red spectroscopy (FTIR), atomic force (AFM) and optical microscopy (OM). The electrochemical indication that the Cys-GA-GOx film on Au surface is not compact and that there were some bare regions which remain catalytically active is supported by AFM and OM results. The construction and the nature of bonding of Au-Cys-GA-GOx biosensor layers is confirmed by the FTIR study.
Кључне речи:
Biosensor / Electrochemical glucose sensing / Microstructural characterization / FTIR / AFM / OM microstructural characterizationИзвор:
International Journal of Electrochemical Science, 2018, 13, 12, 12340-12348Издавач:
- Electrochemical Science Group, Beograd
Финансирање / пројекти:
- Проучавање синтезе, структуре и активности органских једињења природног и синтетског порекла (RS-MESTD-Basic Research (BR or ON)-172013)
- Нов приступ дизајнирању материјала за конверзију и складиштење енергије (RS-MESTD-Basic Research (BR or ON)-172060)
DOI: 10.20964/2018.12.59
ISSN: 1452-3981
WoS: 000452744500080
Scopus: 2-s2.0-85058157122
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Lović, Jelena AU - Stevanović, Sanja AU - Anđelković, Boban D. AU - Petrović, Slobodan AU - Vuković, Dragan AU - Prlainović, Nevena AU - Mijin, Dušan AU - Nikolić, Nebojša AU - Avramov-Ivić, Milka PY - 2018 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3907 AB - Glucose biosensor containing cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is constructed and already electrochemically tested. Now, the electrochemical behavior of biosensor in human serum is further investigated and supported by morphological characterization of layers for the first time. The morphology and microstructure of layers was examined by Fourier transformed infra red spectroscopy (FTIR), atomic force (AFM) and optical microscopy (OM). The electrochemical indication that the Cys-GA-GOx film on Au surface is not compact and that there were some bare regions which remain catalytically active is supported by AFM and OM results. The construction and the nature of bonding of Au-Cys-GA-GOx biosensor layers is confirmed by the FTIR study. PB - Electrochemical Science Group, Beograd T2 - International Journal of Electrochemical Science T1 - Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode EP - 12348 IS - 12 SP - 12340 VL - 13 DO - 10.20964/2018.12.59 ER -
@article{ author = "Lović, Jelena and Stevanović, Sanja and Anđelković, Boban D. and Petrović, Slobodan and Vuković, Dragan and Prlainović, Nevena and Mijin, Dušan and Nikolić, Nebojša and Avramov-Ivić, Milka", year = "2018", abstract = "Glucose biosensor containing cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is constructed and already electrochemically tested. Now, the electrochemical behavior of biosensor in human serum is further investigated and supported by morphological characterization of layers for the first time. The morphology and microstructure of layers was examined by Fourier transformed infra red spectroscopy (FTIR), atomic force (AFM) and optical microscopy (OM). The electrochemical indication that the Cys-GA-GOx film on Au surface is not compact and that there were some bare regions which remain catalytically active is supported by AFM and OM results. The construction and the nature of bonding of Au-Cys-GA-GOx biosensor layers is confirmed by the FTIR study.", publisher = "Electrochemical Science Group, Beograd", journal = "International Journal of Electrochemical Science", title = "Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode", pages = "12348-12340", number = "12", volume = "13", doi = "10.20964/2018.12.59" }
Lović, J., Stevanović, S., Anđelković, B. D., Petrović, S., Vuković, D., Prlainović, N., Mijin, D., Nikolić, N.,& Avramov-Ivić, M.. (2018). Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode. in International Journal of Electrochemical Science Electrochemical Science Group, Beograd., 13(12), 12340-12348. https://doi.org/10.20964/2018.12.59
Lović J, Stevanović S, Anđelković BD, Petrović S, Vuković D, Prlainović N, Mijin D, Nikolić N, Avramov-Ivić M. Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode. in International Journal of Electrochemical Science. 2018;13(12):12340-12348. doi:10.20964/2018.12.59 .
Lović, Jelena, Stevanović, Sanja, Anđelković, Boban D., Petrović, Slobodan, Vuković, Dragan, Prlainović, Nevena, Mijin, Dušan, Nikolić, Nebojša, Avramov-Ivić, Milka, "Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode" in International Journal of Electrochemical Science, 13, no. 12 (2018):12340-12348, https://doi.org/10.20964/2018.12.59 . .