Приказ основних података о документу

dc.creatorDurdević, Sanja
dc.creatorŠavikin, Katarina
dc.creatorŽivković, Jelena Č.
dc.creatorBoehm, Volker
dc.creatorStanojković, Tatjana
dc.creatorDamjanović, Ana
dc.creatorPetrović, Slobodan
dc.date.accessioned2021-03-10T13:48:16Z
dc.date.available2021-03-10T13:48:16Z
dc.date.issued2018
dc.identifier.issn0896-8446
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/3961
dc.description.abstractPomegranate seeds are an excellent source of compounds with biological activity. In the present study, the pomegranate seed oil (PSO) was tested for antioxidant and cytotoxic potential. PSO was extracted by super-critical fluid extraction with previous treatment of seeds by microwaves and analysed for its fatty acid profile, carotenoid and tocopherol contents. PSO demonstrated to be a good source of polyunsaturated fatty acids (PUFAs) (83.5%), followed by monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) (9.5% and 7.0%, respectively). Concerning fatty acid profile, punicic (C18:3), oleic (C18:1) and linoleic (C18:2) acids were the predominant ones. Carotenoids and tocopherols were identified and quantified by HPLC. Results showed that the PSO was a good source of gamma-tocopherol as dominant among investigated compounds (128.6 mg/100 g). The extract was screened for its antioxidant activity using alpha-TEAC assay. Cytotoxic effect was determined by MTT test against malignant HeLa, LS174 and A549 cells (IC50 = 49.51-91.54 mu g/mL), and against normal MRC-5 and EA.hy926 cells (IC50 gt 200 mu g/mL). The best effect was exhibited on cervix adenocarcinoma cell line while no cytotoxicity was found for normal cells pointing out PSO safety.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46013/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45017/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175011/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Supercritical Fluids
dc.subjectPomegranate seed oilen
dc.subjectPunicic aciden
dc.subjectTocopherolsen
dc.subjectalpha-TEAC assayen
dc.subjectMTT assayen
dc.titleAntioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.en
dc.typearticle
dc.rights.licenseARR
dc.citation.epage232
dc.citation.other133: 225-232
dc.citation.rankM21
dc.citation.spage225
dc.citation.volume133
dc.identifier.doi10.1016/j.supflu.2017.10.021
dc.identifier.scopus2-s2.0-85032260370
dc.identifier.wos000426234000028
dc.type.versionpublishedVersion


Документи

Thumbnail

Овај документ се појављује у следећим колекцијама

Приказ основних података о документу