Novel nano-composite hydrogels with honey effective against multi-resistant clinical strains of Acinetobacter baumannii and Pseudomonas aeruginosa
Abstract
Novel alginate hydrogels with silver nanoparticles (AgNPs) and honey components were produced with the aim to target multidrug-resistant bacterial strains causing nosocomial wound infections. AgNP synthesis was optimized in highly concentrated honey solutions so that a 5-month stable, colloid solution with 50% of honey and similar to 8 nm AgNPs at neutral pH was obtained. The colloid solution was further used to produce nano-composite Ag/alginate hydrogels in different forms (microbeads, microfibers and discs) that retained all AgNPs and high fractions of honey components (40-60%) as determined by the phenol-sulfuric acid and Folin-Ciocalteu methods. The hydrogels were characterized by UV-Vis spectroscopy and Fourier-transform infrared-attenuated total reflectance spectroscopy while the antibacterial activity was investigated against a broad spectrum of Gram-negative and Gram-positive bacteria, including 13 multi-resistant clinical strains of Acinetobacter baumannii, one clinical strai...n of Pseudomonas aeruginosa and one clinical strain of Staphylococcus aureus. At the total released silver concentration of similar to 9 mu g/ml, the hydrogels exhibited strong bactericidal activity against standard and most of the investigated multi-resistant hospital strains with the exemption of 3 clinical strains of A. baumannii in which antibacterial effects were absent. These results reveal the need for further in-depth studies of bacterial resistance mechanisms and, in the same time, potentials of the novel Ag/alginate hydrogels with honey components to combat wound infections and enhance healing as non-sticky, antibacterial, and bioactive dressings.
Keywords:
Honey / Silver nanoparticles / Nano-composite hydrogels / Multi-resistance / Antibacterial activitySource:
Applied Microbiology and Biotechnology, 2019, 103, 20, 8529-8543Publisher:
- Springer, New York
Funding / projects:
DOI: 10.1007/s00253-019-10055-2
ISSN: 0175-7598
PubMed: 31396680
WoS: 000491439700022
Scopus: 2-s2.0-85070332626
Collections
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Stojkovska, Jasmina AU - Petrović, Predrag AU - Jancić, Ivan AU - Milenković, Marina AU - Obradović, Bojana PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4043 AB - Novel alginate hydrogels with silver nanoparticles (AgNPs) and honey components were produced with the aim to target multidrug-resistant bacterial strains causing nosocomial wound infections. AgNP synthesis was optimized in highly concentrated honey solutions so that a 5-month stable, colloid solution with 50% of honey and similar to 8 nm AgNPs at neutral pH was obtained. The colloid solution was further used to produce nano-composite Ag/alginate hydrogels in different forms (microbeads, microfibers and discs) that retained all AgNPs and high fractions of honey components (40-60%) as determined by the phenol-sulfuric acid and Folin-Ciocalteu methods. The hydrogels were characterized by UV-Vis spectroscopy and Fourier-transform infrared-attenuated total reflectance spectroscopy while the antibacterial activity was investigated against a broad spectrum of Gram-negative and Gram-positive bacteria, including 13 multi-resistant clinical strains of Acinetobacter baumannii, one clinical strain of Pseudomonas aeruginosa and one clinical strain of Staphylococcus aureus. At the total released silver concentration of similar to 9 mu g/ml, the hydrogels exhibited strong bactericidal activity against standard and most of the investigated multi-resistant hospital strains with the exemption of 3 clinical strains of A. baumannii in which antibacterial effects were absent. These results reveal the need for further in-depth studies of bacterial resistance mechanisms and, in the same time, potentials of the novel Ag/alginate hydrogels with honey components to combat wound infections and enhance healing as non-sticky, antibacterial, and bioactive dressings. PB - Springer, New York T2 - Applied Microbiology and Biotechnology T1 - Novel nano-composite hydrogels with honey effective against multi-resistant clinical strains of Acinetobacter baumannii and Pseudomonas aeruginosa EP - 8543 IS - 20 SP - 8529 VL - 103 DO - 10.1007/s00253-019-10055-2 ER -
@article{ author = "Stojkovska, Jasmina and Petrović, Predrag and Jancić, Ivan and Milenković, Marina and Obradović, Bojana", year = "2019", abstract = "Novel alginate hydrogels with silver nanoparticles (AgNPs) and honey components were produced with the aim to target multidrug-resistant bacterial strains causing nosocomial wound infections. AgNP synthesis was optimized in highly concentrated honey solutions so that a 5-month stable, colloid solution with 50% of honey and similar to 8 nm AgNPs at neutral pH was obtained. The colloid solution was further used to produce nano-composite Ag/alginate hydrogels in different forms (microbeads, microfibers and discs) that retained all AgNPs and high fractions of honey components (40-60%) as determined by the phenol-sulfuric acid and Folin-Ciocalteu methods. The hydrogels were characterized by UV-Vis spectroscopy and Fourier-transform infrared-attenuated total reflectance spectroscopy while the antibacterial activity was investigated against a broad spectrum of Gram-negative and Gram-positive bacteria, including 13 multi-resistant clinical strains of Acinetobacter baumannii, one clinical strain of Pseudomonas aeruginosa and one clinical strain of Staphylococcus aureus. At the total released silver concentration of similar to 9 mu g/ml, the hydrogels exhibited strong bactericidal activity against standard and most of the investigated multi-resistant hospital strains with the exemption of 3 clinical strains of A. baumannii in which antibacterial effects were absent. These results reveal the need for further in-depth studies of bacterial resistance mechanisms and, in the same time, potentials of the novel Ag/alginate hydrogels with honey components to combat wound infections and enhance healing as non-sticky, antibacterial, and bioactive dressings.", publisher = "Springer, New York", journal = "Applied Microbiology and Biotechnology", title = "Novel nano-composite hydrogels with honey effective against multi-resistant clinical strains of Acinetobacter baumannii and Pseudomonas aeruginosa", pages = "8543-8529", number = "20", volume = "103", doi = "10.1007/s00253-019-10055-2" }
Stojkovska, J., Petrović, P., Jancić, I., Milenković, M.,& Obradović, B.. (2019). Novel nano-composite hydrogels with honey effective against multi-resistant clinical strains of Acinetobacter baumannii and Pseudomonas aeruginosa. in Applied Microbiology and Biotechnology Springer, New York., 103(20), 8529-8543. https://doi.org/10.1007/s00253-019-10055-2
Stojkovska J, Petrović P, Jancić I, Milenković M, Obradović B. Novel nano-composite hydrogels with honey effective against multi-resistant clinical strains of Acinetobacter baumannii and Pseudomonas aeruginosa. in Applied Microbiology and Biotechnology. 2019;103(20):8529-8543. doi:10.1007/s00253-019-10055-2 .
Stojkovska, Jasmina, Petrović, Predrag, Jancić, Ivan, Milenković, Marina, Obradović, Bojana, "Novel nano-composite hydrogels with honey effective against multi-resistant clinical strains of Acinetobacter baumannii and Pseudomonas aeruginosa" in Applied Microbiology and Biotechnology, 103, no. 20 (2019):8529-8543, https://doi.org/10.1007/s00253-019-10055-2 . .