Whey valorization using transgalactosylation activity of immobilized beta-galactosidase
Samo za registrovane korisnike
2019
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Whey represents a significant dairy industry by-product that has recently received due attention based on the rich nutrient composition and significant transformation potential. Hereby, we investigated a possibility of whey lactose bioconversion into prebiotic compounds, galacto-oligosaccharides (GOS) using beta-galactosidase transgalactosylation activity. The results showed that whey could be successfully used for GOS synthesis, since the highest GOS concentration (around 62 g L-1) was obtained batchwise using 40% (w/w) sweet whey powder solution under optimum conditions (50 degrees C, pH 4.5). Nevertheless, an efficient immobilized preparation using methacrylic Lifetech ECR8409 immobilization carrier was developed, enabling additional process improvement and ensuring at least 4 reaction cycles with unchanged yields and 2.5- fold enhanced productivity in comparison to the soluble enzyme. Therefore, this study provides a valuable contribution to the efficient and economical valorizatio...n of whey, which can be further on utilized as functional food and feed constituent.
Ključne reči:
beta-galactosidase / galacto-oligosaccharides / GOS / prebiotics / transgalactosylation / wheyIzvor:
International Journal of Food Science and Technology, 2019, 54, 11, 3074-3082Izdavač:
- Wiley, Hoboken
Finansiranje / projekti:
- Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (RS-46010)
- Primena biotehnoloških metoda u održivom iskorišćenju nus-proizvoda agroindustrije (RS-31035)
DOI: 10.1111/ijfs.14222
ISSN: 0950-5423
WoS: 000490171500012
Scopus: 2-s2.0-85067432307
Kolekcije
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Simović, Milica AU - Milivojević, Ana AU - Ćorović, Marija AU - Banjanac, Katarina AU - Bezbradica, Dejan PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4045 AB - Whey represents a significant dairy industry by-product that has recently received due attention based on the rich nutrient composition and significant transformation potential. Hereby, we investigated a possibility of whey lactose bioconversion into prebiotic compounds, galacto-oligosaccharides (GOS) using beta-galactosidase transgalactosylation activity. The results showed that whey could be successfully used for GOS synthesis, since the highest GOS concentration (around 62 g L-1) was obtained batchwise using 40% (w/w) sweet whey powder solution under optimum conditions (50 degrees C, pH 4.5). Nevertheless, an efficient immobilized preparation using methacrylic Lifetech ECR8409 immobilization carrier was developed, enabling additional process improvement and ensuring at least 4 reaction cycles with unchanged yields and 2.5- fold enhanced productivity in comparison to the soluble enzyme. Therefore, this study provides a valuable contribution to the efficient and economical valorization of whey, which can be further on utilized as functional food and feed constituent. PB - Wiley, Hoboken T2 - International Journal of Food Science and Technology T1 - Whey valorization using transgalactosylation activity of immobilized beta-galactosidase EP - 3082 IS - 11 SP - 3074 VL - 54 DO - 10.1111/ijfs.14222 ER -
@article{ author = "Simović, Milica and Milivojević, Ana and Ćorović, Marija and Banjanac, Katarina and Bezbradica, Dejan", year = "2019", abstract = "Whey represents a significant dairy industry by-product that has recently received due attention based on the rich nutrient composition and significant transformation potential. Hereby, we investigated a possibility of whey lactose bioconversion into prebiotic compounds, galacto-oligosaccharides (GOS) using beta-galactosidase transgalactosylation activity. The results showed that whey could be successfully used for GOS synthesis, since the highest GOS concentration (around 62 g L-1) was obtained batchwise using 40% (w/w) sweet whey powder solution under optimum conditions (50 degrees C, pH 4.5). Nevertheless, an efficient immobilized preparation using methacrylic Lifetech ECR8409 immobilization carrier was developed, enabling additional process improvement and ensuring at least 4 reaction cycles with unchanged yields and 2.5- fold enhanced productivity in comparison to the soluble enzyme. Therefore, this study provides a valuable contribution to the efficient and economical valorization of whey, which can be further on utilized as functional food and feed constituent.", publisher = "Wiley, Hoboken", journal = "International Journal of Food Science and Technology", title = "Whey valorization using transgalactosylation activity of immobilized beta-galactosidase", pages = "3082-3074", number = "11", volume = "54", doi = "10.1111/ijfs.14222" }
Simović, M., Milivojević, A., Ćorović, M., Banjanac, K.,& Bezbradica, D.. (2019). Whey valorization using transgalactosylation activity of immobilized beta-galactosidase. in International Journal of Food Science and Technology Wiley, Hoboken., 54(11), 3074-3082. https://doi.org/10.1111/ijfs.14222
Simović M, Milivojević A, Ćorović M, Banjanac K, Bezbradica D. Whey valorization using transgalactosylation activity of immobilized beta-galactosidase. in International Journal of Food Science and Technology. 2019;54(11):3074-3082. doi:10.1111/ijfs.14222 .
Simović, Milica, Milivojević, Ana, Ćorović, Marija, Banjanac, Katarina, Bezbradica, Dejan, "Whey valorization using transgalactosylation activity of immobilized beta-galactosidase" in International Journal of Food Science and Technology, 54, no. 11 (2019):3074-3082, https://doi.org/10.1111/ijfs.14222 . .